Invitrogen

Grim-19 Monoclonal Antibody (1A8), eBioscience™

1 Published Figure

8 References

View all (15) NDUFA13 antibodies

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

FIGURE: 1 / 2

Grim-19 Antibody (14-9937-82) in WB

Immunoblotting of reduced HeLa cell lysate with 5 µg/mL of Anti-Grim-19 Purified antibody. Bands were visualized using Anti-Mouse IgG HRP.

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Product Details

14-9937-82

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-Dependent

Flow Cytometry (Flow)

View 5 publications 5 publications

In vitro Assay (IV)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1A8

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10597591

Product Specific Information

Description: This 1A8 monoclonal antibody reacts with human, mouse, and rat Grim-19. This 16 kDa protein is expressed in many human tissues, especially in the heart and skeletal muscle. Lower expression levels of Grim-19 can be observed in the liver, kidney, and brain. Originally described as a nuclear protein, reports indicate that Grim-19 is primarily localized to mitochondria as an essential component of complex I of the electron transport chain. Grim-19 expression can be induced by interferon-beta and all-trans-retinoic acid in tumor cell lines, leading to cell death. Finally, Grim-19 has been shown to bind the transactivation domain of STAT3 and inhibit its transcriptional activity. The molecular mechanisms of how Grim-19 regulates STAT3 within the cell remains under active investigation.

Applications Reported: This 1A8 antibody has been reported for use in immunoblotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This 1A8 antibody has been tested by immunoblotting of HeLa cell lysate. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

A novel gene associated with Retinoid-IFN-induced Mortality (GRIM) GRIM-19 gene was identified. Antisense expression of GRIM-19 confers a strong resistance against IFN/RA-induced death by reducing the intracellular levels of GRIM-19 protein. Overexpression of GRIM-19 enhances cell death in response to IFN/RA. GRIM-19 is primarily a nuclear protein whose expression is induced by the IFN/RA combination. These data indicate that GRIM-19 is a novel cell death-regulatory molecule.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Discrete viral E2 lysine residues and scavenger receptor MARCO are required for clearance of circulating alphaviruses.

eLife

Carpentier KS,Davenport BJ,Haist KC,McCarthy MK,May NA,Robison A,Ruckert C,Ebel GD,Morrison TE

14-9937-82 was used in Flow Cytometry to reveal a previously unrecognized innate immune pathway that controls alphavirus viremia and dissemination in vertebrate hosts, ultimately influencing disease severity and likely transmission efficiency.

Wed Oct 09 00:00:00 UTC 2019

Early IL-6 signalling promotes IL-27 dependent maturation of regulatory T cells in the lungs and resolution of viral immunopathology.

PLoS pathogens

Pyle CJ,Uwadiae FI,Swieboda DP,Harker JA

14-9937-82 was used in Flow Cytometry to show that during respiratory virus infection the prototypic inflammatory cytokine IL-6 is a critical anti-inflammatory regulator of viral induced immunopathology in the respiratory tract through its induction of IL-27.

Fri Sep 01 00:00:00 UTC 2017

Disulfide HMGB1 derived from platelets coordinates venous thrombosis in mice.

Blood

Stark K,Philippi V,Stockhausen S,Busse J,Antonelli A,Miller M,Schubert I,Hoseinpour P,Chandraratne S,von Brühl ML,Gaertner F,Lorenz M,Agresti A,Coletti R,Antoine DJ,Heermann R,Jung K,Reese S,Laitinen I,Schwaiger M,Walch A,Sperandio M,Nawroth PP,Reinhardt C,Jäckel S,Bianchi ME,Massberg S

14-9937 was used in Immunofluorescence in immunohistochemistry on frozen tissues to identify blood-derived high-mobility group box 1 protein to be a master regulator of the prothrombotic cascade involving platelets and myeloid leukocytes fostering occlusive deep venous thrombosis formation.

Thu Nov 17 00:00:00 UTC 2016

Immune signatures of protective spleen memory CD8 T cells.

Scientific reports

Brinza L,Djebali S,Tomkowiak M,Mafille J,Loiseau C,Jouve PE,de Bernard S,Buffat L,Lina B,Ottmann M,Rosa-Calatrava M,Schicklin S,Bonnefoy N,Lauvau G,Grau M,Wencker M,Arpin C,Walzer T,Leverrier Y,Marvel J

14-9937 was used in Flow cytometry/Cell sorting to identify a range of qualities uniquely associated with protective memory cells.

Thu Nov 24 00:00:00 UTC 2016

Pneumolysin expression by streptococcus pneumoniae protects colonized mice from influenza virus-induced disease.

Virology

Wolf AI,Strauman MC,Mozdzanowska K,Williams KL,Osborne LC,Shen H,Liu Q,Garlick D,Artis D,Hensley SE,Caton AJ,Weiser JN,Erikson J

14-9937-82 was used in Flow Cytometry to investigate severity of influenza virus response with respiratory microbe presence.

Fri Aug 01 00:00:00 UTC 2014

Anatomically restricted synergistic antiviral activities of innate and adaptive immune cells in the skin.

Cell host & microbe

Hickman HD,Reynoso GV,Ngudiankama BF,Rubin EJ,Magadán JG,Cush SS,Gibbs J,Molon B,Bronte V,Bennink JR,Yewdell JW

14-9937 was used in Flow cytometry/Cell sorting to explore the spatiotemporal organization between antiviral immune cells and vaccinia virus-infected murine cells.

Wed Feb 13 00:00:00 UTC 2013

Monocytes, neutrophils, and platelets cooperate to initiate and propagate venous thrombosis in mice in vivo.

The Journal of experimental medicine

von Brühl ML,Stark K,Steinhart A,Chandraratne S,Konrad I,Lorenz M,Khandoga A,Tirniceriu A,Coletti R,Köllnberger M,Byrne RA,Laitinen I,Walch A,Brill A,Pfeiler S,Manukyan D,Braun S,Lange P,Riegger J,Ware J,Eckart A,Haidari S,Rudelius M,Schulz C,Echtler K,Brinkmann V,Schwaiger M,Preissner KT,Wagner DD,Mackman N,Engelmann B,Massberg S

14-9937 was used in in vivo experiments to investigate a novel mouse model of deep vein thrombosis as a useful tool to study the sequence of disease events.

Mon Apr 09 00:00:00 UTC 2012

The cell death regulator GRIM-19 is an inhibitor of signal transducer and activator of transcription 3.

Proceedings of the National Academy of Sciences of the United States of America

Zhang J,Yang J,Roy SK,Tininini S,Hu J,Bromberg JF,Poli V,Stark GR,Kalvakolanu DV

Published figure using Grim-19 monoclonal antibody (Product # 14-9937-82) in Western Blot

Tue Aug 05 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: Cell d; Cell death regulatory protein GRIM-19; cell death-regulatory protein GRIM19; CI-B16.6; complex I B16.6 subunit; Complex I-B16.6; FLJ58045; FLJ59191; gene associated with retinoic and IFN-induced mortality 19 protein; Gene associated with retinoic and interferon-induced mortality 19 protein; gene associated with retinoic-interferon-induced mortality 19 protein; genes associated with retinoid-IFN-induced mortality 19; GRIM-19; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; NADH-ubiquinone oxidoreductase B16.6 subunit

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Gene Aliases: 2700054G14Rik; AU022060; B16.6; CDA016; CGI-39; GRIM-19; GRIM19; NDUFA13

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UniProt ID: (Human) Q9P0J0, (Mouse) Q9ERS2

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Entrez Gene ID: (Rat) 100911483, (Human) 51079, (Mouse) 67184

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Function(s)

NADH dehydrogenase activity protein binding ATP binding NADH dehydrogenase (ubiquinone) activity

Process(es)

mitochondrial electron transport, NADH to ubiquinone positive regulation of peptidase activity negative regulation of cell growth mitochondrial respiratory chain complex I assembly cellular response to interferon-beta positive regulation of cysteine-type endopeptidase activity involved in apoptotic process protein import into mitochondrial inner membrane positive regulation of protein catabolic process negative regulation of transcription, DNA-templated cellular response to retinoic acid reactive oxygen species metabolic process apoptotic signaling pathway extrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway oxidation-reduction process

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Grim-19 Monoclonal Antibody (1A8), eBioscience™

Grim-19 Antibody (14-9937-82) in WB

Product Details

14-9937-82

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

In vitro Assay (IV)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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