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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Histone 1F0 Antibodies

          Bethyl Laboratories

          H1F0/Histone H1.0 Polyclonal Antibody

          View all (15) Histone 1F0 antibodies
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          Protocols
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          Datasheet
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          Cite H1F0/Histone H1.0 Polyclonal Antibody

          • Antibody Testing Data (2)
          H1F0/Histone H1.0 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          H1F0/Histone H1.0 Antibody in Western Blot (WB)
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          FIGURE: 1 / 2

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          H1F0/Histone H1.0 Antibody (A305-019A) in WB

          Detection of human and mouse H1F0/Histone H1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-H1F0/Histone H1.0 antibody (Product # A305-019A lot 1) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          H1F0/Histone H1.0 Antibody in Western Blot (WB)
          H1F0/Histone H1.0 Antibody in Immunoprecipitation (IP)

          Product Details

          A305-019A

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:5,000
          -

          Immunoprecipitation (IP)

          2-10 µg/mg lysate
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Region between residue 75 to 125 of human Histone H1.0.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          tris citrate/phosphate, pH 7-8

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          The recommended shelf life for this product is 1 year from date of receipt.

          Based on 100% sequence identity, this antibody is predicted to react with Orangutan

          Target Information

          Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

          Have you cited this product in a publication?
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          Cite this product

          Bioinformatics

          Protein Aliases: H1 histone family, member 0; H1-0 histone; H1.0, H1(0), H1-0; Histone H1'; Histone H1(0); Histone H1.0; MyD196

          View more View less

          Gene Aliases: D130017D06Rik; H1(0); H1-0; H10; H1F0; H1FV

          View more View less

          UniProt ID: (Human) P07305, (Mouse) P10922

          View more View less

          Entrez Gene ID: (Human) 3005, (Mouse) 14958

          View more View less

          Function(s)
          protein binding chromatin DNA binding poly(A) RNA binding DNA binding
          Process(es)
          nucleosome assembly apoptotic DNA fragmentation
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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