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H3K18ac Antibody (PA5-85523) in ICC/IF
Histone H3 (acetyl Lys18) antibody detects Histone H3 (acetyl Lys18) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 (acetyl Lys18) protein stained by Histone H3 (acetyl Lys18) antibody (Product # PA5-85523) diluted at 1:500. Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by (Product # MA5-31466) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-85523
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Yeast
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide surrounding acetyl Lys18 of human Histone H3. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2792663
Product Specific Information
Keep as concentrated solution.
Positive Control: HeLa.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Click here for Master Lot linking information.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Acetyl H3; Acetyl Histone H3; Acetyl-H3 K18; Acetyl-H3 Lys18; Acetyl-Histone H3 K18; Acetyl-Histone H3 Lys18; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K18ac; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3K18; H3Lys18ac; Hht1p; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 2, H3c2, pseudogene; histone gene complex 1; Histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K18
Gene Aliases: BUR5; H3; H3-143; H3-291; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; HHT1; HHT2; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461; N2749; SIN2; YBR010W; YBR0201; YNL031C
positive regulation of defense response to virus by host
nucleosome assembly
DNA replication-dependent nucleosome assembly
protein heterotetramerization
regulation of gene silencing
chromatin silencing at rDNA
blood coagulation
gene silencing by RNA
cellular protein metabolic process
negative regulation of gene expression, epigenetic
positive regulation of gene expression, epigenetic
telomere organization
cell-cell adhesion
xenophagy
DNA repair
chromatin assembly or disassembly
cellular response to DNA damage stimulus
mitotic spindle assembly checkpoint
rRNA transcription
sexual sporulation resulting in formation of a cellular spore
global genome nucleotide-excision repair
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