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Rockland
H3K18me3 Polyclonal Antibody
FIGURE: 1 / 4
H3K18me3 Antibody (600-401-I80) in ICC/IF
Immunofluorescence of Rabbit Anti-Histone H3 [Trimethyl Lys18] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Trimethyl Lys18] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Trimethyl Lys18] is nuclear and chromosomal. Staining: Histone H3 [Trimethyl Lys18] is expressed in green, nuclei are counterstained with DAPI (blue).
Product Details
600-401-I80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
C. elegans,
Chicken,
Fruit fly,
Human,
Mouse,
Plant,
Rat,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Histone H3 [Trimethyl Lys18] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 18 of human Histone H3.2.
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Histone H3 [Trimethyl Lys18] reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CG5825-PA; CG5825-PC; CG5825-PD; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 histone, family 3B (H3.3B); H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3.A; H3.B; H3K56me3; H3L-like histone; H3Lys18me3; H3Lys56me3; His3.3A-PA; His3.3A-PC; His3.3A-PD; histone 1, H3b; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; histone cluster 2, H3c2, pseudogene; Histone H2A; Histone H3.1; Histone H3.2; Histone H3.3; histone H3.3 variant; Histone H3.3A; Histone H3.3B; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; histone variant 3.3; histone variant H3.3; Tri-methyl H3; Tri-methyl Histone H3; Trimethyl H3; Trimethyl Histone H3
Gene Aliases: B0035.7; CG5825; CG8989; dH3.3A; Dmel\CG5825; Dmel_CG5825; F07B7.10; F07B7.3; F08G2.2; F17E9.13; F35H10.1; F45F2.4; F54E12.5; F55G1.10; H02I12.7; H3; H3-143; H3-3A; H3-3B; H3-53; H3-614; H3-B; H3-F; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3.3; H3.3A; H3.3B; H3/l; H3/M; H3/n; H3/o; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F2; H3F3; H3F3A; H3F3B; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; his-12; his-16; his-19; his-21; his-3; His-3.3A; his-30; his-33; his-43; his-47; his-51; his-53; his-57; his-61; his-65; his-68; his-7; His3.3; His3.3A; His3.3B; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; K06C4.11; K06C4.3; M32461; PH3; PP781; T10C6.12; T23D8.6; ZK131.10; ZK131.6
UniProt ID: (Human) P84243, (Human) P68431, (Human) Q71DI3, (Fruit fly) C0HL66, (Mouse) P84228, (C. elegans) P09588
Entrez Gene ID: (Human) 3021, (Human) 8358, (Human) 333932, (Human) 126961, (Fruit fly) 33736, (Mouse) 319150, (Mouse) 97114, (C. elegans) 180073
chromatin silencing at rDNA
nucleosome assembly
blood coagulation
gene silencing by RNA
cellular protein metabolic process
negative regulation of gene expression, epigenetic
positive regulation of gene expression, epigenetic
positive regulation of defense response to virus by host
DNA replication-dependent nucleosome assembly
protein heterotetramerization
regulation of gene silencing
chromatin assembly or disassembly
male meiosis
cell adhesion
regulation of cell shape
chromatin organization
DNA replication-independent nucleosome assembly
brain development
response to hormone
positive regulation of cell growth
telomere organization
cell-cell adhesion
xenophagy
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