Invitrogen

H3K4me1 Recombinant Superclonal™ Antibody, ChIP-Verified

H3K4me1 Antibody in Immunocytochemistry (ICC/IF)

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H3K4me1 Antibody (710795) in ICC/IF

Immunofluorescence was performed on fixed and permeabilized Hela cells for detection of Histone H3K4me1 using Anti-Histone H3K4me1 Recombinant Rabbit Superclonal™ Antibody (Product # 710795, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of Histone H3K4me1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mounta... View More

H3K4me1 Antibody in ChIP-Sequencing (ChIP-seq)

H3K4me1 Antibody in Peptide array (ARRAY)

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Product Details

710795

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL

Flow Cytometry (Flow)

View 1 publication 1 publication

ChIP assay (ChIP)

1-5 µg

View 3 publications 3 publications

ChIP-sequencing (ChIP-Seq)

3 µg

Peptide Array (Array)

0.25 µg/mL

CUT&RUN (C&R)

1:100

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Immunogen

Methylated peptide (Lys4) corresponding to human Histone H3 (aa 2-11)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532764

Product Specific Information

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Scalable single-cell profiling of chromatin modifications with sciCUT&Tag.

Nature protocols

Janssens DH,Greene JE,Wu SJ,Codomo CA,Minot SS,Furlan SN,Ahmad K,Henikoff S

710795 was used in Flow Cytometry to describe a combinatorial barcoding strategy for CUT&Tag that harnesses a nanowell dispenser for simple, high-resolution, high-throughput, single-cell chromatin profiling.

Mon Jan 01 00:00:00 UTC 2024

Nuclear RIPK1 promotes chromatin remodeling to mediate inflammatory response.

Cell research

Li W,Shan B,Zou C,Wang H,Zhang MM,Zhu H,Naito MG,Xu D,Manuel VJ,Mifflin L,Hou Z,Ravits J,Yuan J

710795 was used in Immunohistochemistry (Frozen) to suggest that RIPK1 kinase serves as a transcriptional coregulator in nucleus that can transmit extracellular stimuli to the BAF complex to modulate chromatin accessibility and directly regulate the transcription of specific genes involved in mediating inflammatory responses.

Fri Jul 01 00:00:00 UTC 2022

STAT3-mediated allelic imbalance of novel genetic variant Rs1047643 and B-cell-specific super-enhancer in association with systemic lupus erythematosus.

eLife

Zhang Y,Day K,Absher DM

710795 was used in ChIP assay to reveal that STAT3-mediated SE activity and cis-regulatory effects of SNP rs1047643 at 8p23 locus are associated with B cell deregulation in SLE.

Mon Feb 21 00:00:00 UTC 2022

STAT3-mediated allelic imbalance of novel genetic variant Rs1047643 and B-cell-specific super-enhancer in association with systemic lupus erythematosus.

eLife

Zhang Y,Day K,Absher DM

710795 was used in ChIP assay to reveal that STAT3-mediated SE activity and cis-regulatory effects of SNP rs1047643 at 8p23 locus are associated with B cell deregulation in SLE.

Mon Feb 21 00:00:00 UTC 2022

Automated CUT&Tag profiling of chromatin heterogeneity in mixed-lineage leukemia.

Nature genetics

Janssens DH,Meers MP,Wu SJ,Babaeva E,Meshinchi S,Sarthy JF,Ahmad K,Henikoff S

710795 was used in ChIP assay to identify common and tumor-subtype-specific sites of aberrant regulation induced by KMT2A oncofusion proteins.

Mon Nov 01 00:00:00 UTC 2021

Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies.

Molecular cell

Shah RN,Grzybowski AT,Cornett EM,Johnstone AL,Dickson BM,Boone BA,Cheek MA,Cowles MW,Maryanski D,Meiners MJ,Tiedemann RL,Vaughan RM,Arora N,Sun ZW,Rothbart SB,Keogh MC,Ruthenburg AJ

Thu Oct 04 00:00:00 UTC 2018

Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies.

Molecular cell

Shah RN,Grzybowski AT,Cornett EM,Johnstone AL,Dickson BM,Boone BA,Cheek MA,Cowles MW,Maryanski D,Meiners MJ,Tiedemann RL,Vaughan RM,Arora N,Sun ZW,Rothbart SB,Keogh MC,Ruthenburg AJ

Thu Oct 04 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K4; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3K4; H3K4me; H3Lys4me; H3Lys4me1; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 2, H3c2, pseudogene; histone gene complex 1; Histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K4me1; methyl Histone 3; methyl Histone H3; Methyl-H3 K4; Methyl-H3 Lys4; Methyl-Histone H3 K4; Methyl-Histone H3 Lys4; Mono-methyl H3; Mono-methyl Histone H3

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Gene Aliases: H3; H3-143; H3-291; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461

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UniProt ID: (Human) P68431, (Human) Q71DI3, (Mouse) P68433, (Mouse) P84228, (Mouse) A3KMN6

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Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Mouse) 360198, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114

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Function(s)

DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion

Process(es)

chromatin silencing at rDNA nucleosome assembly blood coagulation gene silencing by RNA cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic positive regulation of defense response to virus by host DNA replication-dependent nucleosome assembly protein heterotetramerization regulation of gene silencing telomere organization cell-cell adhesion xenophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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H3K4me1 Recombinant Superclonal™ Antibody, ChIP-Verified

H3K4me1 Antibody (710795) in ICC/IF

Product Details

710795

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

Peptide Array (Array)

CUT&RUN (C&R)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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