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Invitrogen
H4ac pan-acetyl (K5,K8,K12,K16) Monoclonal Antibody (3HH4-2C2)
FIGURE: 1 / 4
H4ac pan-acetyl (K5,K8,K12,K16) Antibody (MA3-066) in ICC/IF
Immunofluorescent analysis of pan-acetyl Histone H4 (green) in HeLa cells. HeLa cells were treated with 10 mM sodium butyrate for 6 hours before fixing with 2% formaldehyde (Product # 28908) for 5 min, permeabilized with 1X Permeablilization buffer (Product # 8408400) for 15 minutes, and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with pan-acetyl Hist... View More
Product Details
MA3-066
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3HH4-2C2
Immunogen
Ovalbumin conjugated synthetic peptide (MSGRG(acetyl-K)GG(acetyl-K)GLG(acetyl-K)GGA(acetyl-K)RHRKVLRC) corresponding to residues 1 to 24 of human histone H4
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2633028
Product Specific Information
MA3-066 detects acetylated histone H4 at Lysines 5, 8, 12 or 16 in human samples
MA3-066 has successfully been used in western blot, immunofluoresecence and immunoprecipitation applications.
Immunogen is a synthetic peptide conjugated to ovalbumin corresponding to residues 1 to 24 of human histone H4 which is conserved in a wide range of species.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acetyl Histone H4; Acetyl-H4 K5 K8 K12 K16; Acetyl-H4 Lys5 Lys8 Lys12 Lys16; Acetyl-Histone H4 K5 K8 K12 K16; Acetyl-Histone H4 Lys5 Lys8 Lys12 Lys16; H4 ac pan-acetyl; H4 histone family, member A; H4 histone family, member E; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4ac; H4K12; H4K12a; H4K12ac; H4K16; H4K16ac; H4K5; H4K5,8,12,16ac; H4K5ac; H4K8; H4K8ac; H4Lys5,8,12,16ac; HIST1H4; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4j; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; Histone Cluster 4; histone family member; Histone H4; Histone H4 acetyl; Histone H4A; Histone H4K12; Histone H4K16; Histone H4K5; Histone H4K8; histone IV, family 2
Gene Aliases: dJ160A22.2; FO108; H4; H4-16; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4
UniProt ID: (Human) P62805
Entrez Gene ID: (Human) 8359, (Human) 8366, (Human) 8367, (Human) 8294, (Human) 8363, (Human) 8370, (Human) 121504
chromatin silencing at rDNA
double-strand break repair via nonhomologous end joining
nucleosome assembly
DNA replication-dependent nucleosome assembly
DNA replication-independent nucleosome assembly
DNA-templated transcription, initiation
telomere capping
gene silencing by RNA
telomere organization
CENP-A containing nucleosome assembly
cellular protein metabolic process
negative regulation of megakaryocyte differentiation
negative regulation of gene expression, epigenetic
positive regulation of gene expression, epigenetic
protein heterotetramerization
beta-catenin-TCF complex assembly
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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