Invitrogen

HA Tag Monoclonal Antibody (16B12), Alexa Fluor™ 594

HA Tag Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

HA Tag Antibody (A-21288) in ICC/IF

Immunofluorescent analysis of HA Tag was performed using 70% confluent HA-H3 transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HA Tag Monoclonal Antibody (16B12), Alexa Fluor 594 (Product # A-21288) at 5 µg/mL and Histone H3 Rabbit Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey an... View More

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Product Details

A-21288

Applications

Tested Dilution

Publications

Western Blot (WB)

1-10 µg/mL

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:200

View 4 publications 4 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

16B12

Immunogen

twelve amino acid peptide CYPYDVPDYASL.

Conjugate

Alexa Fluor™ 594 Alexa Fluor™ 594 Alexa Fluor™ 594

Excitation/Emission Max

590/618 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2, with 0.1% BSA

Contains

5mM sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2535830

Target Information

HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

^αN-Acetyl β-Endorphin Is an Endogenous Ligand of σ1Rs That Regulates Mu-Opioid Receptor Signaling by Exchanging G Proteins for σ2Rs in σ1R Oligomers.

International journal of molecular sciences

Garzón-Niño J,Cortés-Montero E,Rodríguez-Muñoz M,Sánchez-Blázquez P

A-21288 was used in Immunocytochemistry, Western Blot, Immunohistochemistry to suggest that the pharmacological ß-Endorphin-specific epsilon receptor is a s1R-regulated MOR and that ß-Endorphin and aN-acetyl ß-Endorphin are endogenous ligands of s1R.

Thu Dec 29 00:00:00 UTC 2022

The Burkholderia pseudomallei intracellular 'TRANSITome'.

Nature communications

Heacock-Kang Y,McMillan IA,Norris MH,Sun Z,Zarzycki-Siek J,Bluhm AP,Cabanas D,Norton RE,Ketheesan N,Miller JF,Schweizer HP,Hoang TT

Published figure using HA Tag monoclonal antibody (Product # A-21288) in Immunocytochemistry

Fri Mar 26 00:00:00 UTC 2021

Cardiovascular response to small-molecule APJ activation.

JCI insight

Ason B,Chen Y,Guo Q,Hoagland KM,Chui RW,Fielden M,Sutherland W,Chen R,Zhang Y,Mihardja S,Ma X,Li X,Sun Y,Liu D,Nguyen K,Wang J,Li N,Rajamani S,Qu Y,Gao B,Boden A,Chintalgattu V,Turk JR,Chan J,Hu LA,Dransfield P,Houze J,Wong J,Ma J,Pattaropong V,Véniant MM,Vargas HM,Swaminath G,Khakoo AY

A-21288 was used in Immunocytochemistry-immunoflourescence to demonstrate the feasibility of developing clinical, viable, potent small-molecule agonists that mimic the endogenous APJ ligand with more favorable drug-like properties and highlights potential limitations for APJ agonism for this indication.

Thu Apr 23 00:00:00 UTC 2020

High mitogenic stimulation arrests angiogenesis.

Nature communications

Pontes-Quero S,Fernández-Chacón M,Luo W,Lunella FF,Casquero-Garcia V,Garcia-Gonzalez I,Hermoso A,Rocha SF,Bansal M,Benedito R

A-21288 was used in Immunohistochemistry to study the effects of high mitogenic stimulation on angiogenesis in different vascular contexts.

Wed May 01 00:00:00 UTC 2019

Exosomal Tat protein activates latent HIV-1 in primary, resting CD4+ T lymphocytes.

JCI insight

Tang X,Lu H,Dooner M,Chapman S,Quesenberry PJ,Ramratnam B

A-21288 was used in Immunocytochemistry to study the utility of CD4+ receptor targeting exosomes as an HIV-1 latency reversal agent.

Thu Apr 05 00:00:00 UTC 2018

Bias Factor and Therapeutic Window Correlate to Predict Safer Opioid Analgesics.

Cell

Schmid CL,Kennedy NM,Ross NC,Lovell KM,Yue Z,Morgenweck J,Cameron MD,Bannister TD,Bohn LM

A-21288 was used in Flow Cytometry to characterise the structural features of a series of mu-opioid-receptor-selective agonists that preferentially activate receptors to couple to G proteins or to recruit βarrestin proteins.

Thu Nov 16 00:00:00 UTC 2017

Mitochondrial CaMKII inhibition in airway epithelium protects against allergic asthma.

JCI insight

Sebag SC,Koval OM,Paschke JD,Winters CJ,Jaffer OA,Dworski R,Sutterwala FS,Anderson ME,Grumbach IM

A-21288 was used in Immunohistochemistry-immunofluorescence to identify a pivotal role for mitochondrial Ca(2+)/calmodulin-dependent protein kinase II in reactive oxygen species generation, eosinophilic inflammation and airway hyperreactivity in allergic asthma.

Thu Feb 09 00:00:00 UTC 2017

Apicoplast targeting of a Toxoplasma gondii transmembrane protein requires a cytosolic tyrosine-based motif.

Traffic (Copenhagen, Denmark)

DeRocher AE,Karnataki A,Vaney P,Parsons M

A-21288 was used in Immunocytochemistry-immunoflourescence to identify a short motif prior to the first transmembrane domain of the Toxoplasma gondii apicoplast phosphate transporter 1 that is necessary for apicoplast trafficking.

Tue May 01 00:00:00 UTC 2012

Characterization of the CaMKKβ-AMPK signaling complex.

Cellular signalling

Green MF,Anderson KA,Means AR

A-21288 was used in immunocytochemistry to elucidate the CaMKKβ-AMPK signaling complex.

Thu Dec 01 00:00:00 UTC 2011

Conserved SOL-1 proteins regulate ionotropic glutamate receptor desensitization.

Proceedings of the National Academy of Sciences of the United States of America

Walker CS,Francis MM,Brockie PJ,Madsen DM,Zheng Y,Maricq AV

A-21288 was used in immunohistochemistry to analyze regulation of ionotropic glutamate receptor desensitization by conserved SOL-1 proteins

Tue Jul 11 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: HA; HA Epitope Tag; HA Tag; HA-tag; hemagglutinin

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HA Tag Monoclonal Antibody (16B12), Alexa Fluor™ 594

HA Tag Antibody (A-21288) in ICC/IF

Product Details

A-21288

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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