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GenScript
HA tag Recombinant Rabbit Monoclonal Antibody (109B2), MonoRab™
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FIGURE: 1 / 2
HA tag Antibody (A01963-40) in ICC/IF
Immunofluorescence staining of MonoRab™ HA tag Antibody (109B2), mAb, Rabbit (A01963-40) with HA-tagged red fluorescent protein expression in CHO cells. The cells were fixed with 4% Poly-Formaldehyde for 5min, permeabilized with 0. 1% TritonX-100 for 5 minutes, and blocked in 3% BSA for 30min at room temperature. The cells were then stained with 1/1000 Rabbit Anti-HA-tag mAb at room temperature for 2h, followed by a further incubation at 37 °C for 1h with Mouse Anti-Rabbit IgG Antibody [iFluor488], mAb at 5 µg/mL.
Product Details
A01963-40
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
/ IgG
Expression System
Mammalian cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
109B2
Immunogen
a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). conjugated to KLH
Conjugate
Form
Lyophilized
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRab™ technology to generate high affinity and specific monoclonal rabbit antibodies. The HA tag is composed of several amino acids containing YPYDVPDYA and does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. GenScript MonoRab™ HA tag Antibody (109B2), mAb, Rabbit is specific to HA tags placed at the N-terminal, C-terminal, and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify and detect HA-tagged fusion proteins in bacteria, insect cells, and mammalian cells.
Target Information
HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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