Proteintech

HADHA Polyclonal Antibody

HADHA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 31

HADHA Antibody (10758-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 10758-1-AP (HADHA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

HADHA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HADHA Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10758-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HADHA Fusion Protein Ag1211 (339-763 aa encoded by BC009235)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LMGLYHGQV LCKKNKFGAP QKDVKHLAIL GAGLMGAGIA QVSVDKGLKT ILKDATLTAL DRGQQQVFKG LNDKVKKKAL TSFERDSIFS NLTGQLDYQG FEKADMVIEA VFEDLSLKHR VLKEVEAVIP DHCIFASNTS ALPISEIAAV SKRPEKVIGM HYFSPVDKMQ LLEIITTEKT SKDTSASAVA VGLKQGKVII VVKDGPGFYT TRCLAPMMSE VIRILQEGVD PKKLDSLTTS FGFPVGAATL VDEVGVDVAK HVAEDLGKVF GERFGGGNPE LLTQMVSKGF LGRKSGKGFY IYQEGVKRKD LNSDMDSILA SLKLPPKSEV SSDEDIQFRL VTRFVNEAVM CLQEGILATP AEGDIGAVFG LGFPPCLGGP FRFVDLYGAQ KIVDRLKKYE AAYGKQFTPC QLLADHANSP NKKFYQ (339-763 aa encoded by BC009235)

Target Information

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

G-protein coupled receptor 19 (GPR19) knockout mice display sex-dependent metabolic dysfunction.

Scientific reports

Mushala BAS,Xie B,Sipula IJ,Stoner MW,Thapa D,Manning JR,Bugga P,Vandevender AM,Jurczak MJ,Scott I

Sat Apr 15 00:00:00 UTC 2023

Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging.

Frontiers in cell and developmental biology

Zhang H,Yan M,Liu T,Wei P,Chai N,Li L,Wang J,Yu X,Lin Y,Qiu B,Zhao Y

Tue Apr 05 00:00:00 UTC 2022

3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol) Counteracts ERK1/2 and mTOR Activation, Pro-Inflammatory Cytokine Release, Autophagy and Mitophagy Reduction Mediated by Benzo[a]pyrene in Primary Human Colonic Epithelial Cells.

Pharmaceutics

Santarelli R,Pompili C,Gilardini Montani MS,Evangelista L,Gonnella R,Cirone M

Thu Mar 17 00:00:00 UTC 2022

The Impaired Bioenergetics of Diabetic Cardiac Microvascular Endothelial Cells.

Frontiers in endocrinology

Zhang H,Shen Y,Kim IM,Weintraub NL,Tang Y

Fri Jan 14 00:00:00 UTC 2022

Feedback repression of PPARα signaling by Let-7 microRNA.

Cell reports

Yagai T,Yan T,Luo Y,Takahashi S,Aibara D,Kim D,Brocker CN,Levi M,Motohashi H,Gonzalez FJ

Tue Aug 10 00:00:00 UTC 2021

Cardioprotective Role of SIRT5 in Response to Acute Ischemia Through a Novel Liver-Cardiac Crosstalk Mechanism.

Frontiers in cell and developmental biology

Zhou B,Xiao M,Hu H,Pei X,Xue Y,Miao G,Wang J,Li W,Du Y,Zhang P,Wei T

Tue Aug 10 00:00:00 UTC 2021

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Oxidative medicine and cellular longevity

Wang W,Hu X,Xia Z,Liu Z,Zhong Z,Lu Z,Liu A,Ye S,Cao Q,Wang Y,Zhu F,Ye Q

Wed Dec 16 00:00:00 UTC 2020

High-Dose Dexamethasone Manipulates the Tumor Microenvironment and Internal Metabolic Pathways in Anti-Tumor Progression.

International journal of molecular sciences

Xu L,Xia H,Ni D,Hu Y,Liu J,Qin Y,Zhou Q,Yi Q,Xie Y

Sat Mar 07 00:00:00 UTC 2020

Rescue of myocardial energetic dysfunction in diabetes through the correction of mitochondrial hyperacetylation by honokiol.

JCI insight

Kerr M,Miller JJ,Thapa D,Stiewe S,Timm KN,Aparicio CNM,Scott I,Tyler DJ,Heather LC

Thu Sep 03 00:00:00 UTC 2020

The protein acetylase GCN5L1 modulates hepatic fatty acid oxidation activity via acetylation of the mitochondrial β-oxidation enzyme HADHA.

The Journal of biological chemistry

Thapa D,Wu K,Stoner MW,Xie B,Zhang M,Manning JR,Lu Z,Li JH,Chen Y,Gucek M,Playford MP,Mehta NN,Harmon D,O'Doherty RM,Jurczak MJ,Sack MN,Scott I

Fri Nov 16 00:00:00 UTC 2018

The protein acetylase GCN5L1 modulates hepatic fatty acid oxidation activity via acetylation of the mitochondrial β-oxidation enzyme HADHA.

The Journal of biological chemistry

Thapa D,Wu K,Stoner MW,Xie B,Zhang M,Manning JR,Lu Z,Li JH,Chen Y,Gucek M,Playford MP,Mehta NN,Harmon D,O'Doherty RM,Jurczak MJ,Sack MN,Scott I

Fri Nov 16 00:00:00 UTC 2018

Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.

Molecular medicine reports

Zhang J,Zhao J,Zheng X,Cai K,Mao Q,Xia H

Thu Feb 01 00:00:00 UTC 2018

Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: Therapeutic effects of ginsenoside Rb1.

Biochimica et biophysica acta. Molecular basis of disease

Li J,Yang YL,Li LZ,Zhang L,Liu Q,Liu K,Li P,Liu B,Qi LW

Wed Nov 01 00:00:00 UTC 2017

Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: Therapeutic effects of ginsenoside Rb1.

Biochimica et biophysica acta. Molecular basis of disease

Li J,Yang YL,Li LZ,Zhang L,Liu Q,Liu K,Li P,Liu B,Qi LW

Wed Nov 01 00:00:00 UTC 2017

Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.

American journal of physiology. Heart and circulatory physiology

Thapa D,Zhang M,Manning JR,Guimarães DA,Stoner MW,O'Doherty RM,Shiva S,Scott I

Tue Aug 01 00:00:00 UTC 2017

Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.

American journal of physiology. Heart and circulatory physiology

Thapa D,Zhang M,Manning JR,Guimarães DA,Stoner MW,O'Doherty RM,Shiva S,Scott I

Tue Aug 01 00:00:00 UTC 2017

Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.

Cell

Yang W,Nagasawa K,Münch C,Xu Y,Satterstrom K,Jeong S,Hayes SD,Jedrychowski MP,Vyas FS,Zaganjor E,Guarani V,Ringel AE,Gygi SP,Harper JW,Haigis MC

Thu Nov 03 00:00:00 UTC 2016

Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.

Cell

Yang W,Nagasawa K,Münch C,Xu Y,Satterstrom K,Jeong S,Hayes SD,Jedrychowski MP,Vyas FS,Zaganjor E,Guarani V,Ringel AE,Gygi SP,Harper JW,Haigis MC

Thu Nov 03 00:00:00 UTC 2016

Effect of high-fat diet on hepatic proteomics of hamsters.

Journal of agricultural and food chemistry

Liao CC,Lin YL,Kuo CF

Wed Feb 18 00:00:00 UTC 2015

Effect of high-fat diet on hepatic proteomics of hamsters.

Journal of agricultural and food chemistry

Liao CC,Lin YL,Kuo CF

Wed Feb 18 00:00:00 UTC 2015

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Cell metabolism

Richter-Dennerlein R,Korwitz A,Haag M,Tatsuta T,Dargazanli S,Baker M,Decker T,Lamkemeyer T,Rugarli EI,Langer T

Tue Jul 01 00:00:00 UTC 2014

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Cell metabolism

Richter-Dennerlein R,Korwitz A,Haag M,Tatsuta T,Dargazanli S,Baker M,Decker T,Lamkemeyer T,Rugarli EI,Langer T

Tue Jul 01 00:00:00 UTC 2014

The trifunctional protein mediates thyroid hormone receptor-dependent stimulation of mitochondria metabolism.

Molecular endocrinology (Baltimore, Md.)

Chocron ES,Sayre NL,Holstein D,Saelim N,Ibdah JA,Dong LQ,Zhu X,Cheng SY,Lechleiter JD

Sun Jul 01 00:00:00 UTC 2012

The trifunctional protein mediates thyroid hormone receptor-dependent stimulation of mitochondria metabolism.

Molecular endocrinology (Baltimore, Md.)

Chocron ES,Sayre NL,Holstein D,Saelim N,Ibdah JA,Dong LQ,Zhu X,Cheng SY,Lechleiter JD

Sun Jul 01 00:00:00 UTC 2012

Pressure load: the main factor for altered gene expression in right ventricular hypertrophy in chronic hypoxic rats.

PloS one

Baandrup JD,Markvardsen LH,Peters CD,Schou UK,Jensen JL,Magnusson NE,Ørntoft TF,Kruhøffer M,Simonsen U

Wed Jan 05 00:00:00 UTC 2011

Pressure load: the main factor for altered gene expression in right ventricular hypertrophy in chronic hypoxic rats.

PloS one

Baandrup JD,Markvardsen LH,Peters CD,Schou UK,Jensen JL,Magnusson NE,Ørntoft TF,Kruhøffer M,Simonsen U

Wed Jan 05 00:00:00 UTC 2011

Pressure load: the main factor for altered gene expression in right ventricular hypertrophy in chronic hypoxic rats.

PloS one

Baandrup JD,Markvardsen LH,Peters CD,Schou UK,Jensen JL,Magnusson NE,Ørntoft TF,Kruhøffer M,Simonsen U

Wed Jan 05 00:00:00 UTC 2011

Pressure load: the main factor for altered gene expression in right ventricular hypertrophy in chronic hypoxic rats.

PloS one

Baandrup JD,Markvardsen LH,Peters CD,Schou UK,Jensen JL,Magnusson NE,Ørntoft TF,Kruhøffer M,Simonsen U

Wed Jan 05 00:00:00 UTC 2011

Men supplemented with 17beta-estradiol have increased beta-oxidation capacity in skeletal muscle.

Physiological genomics

Maher AC,Akhtar M,Tarnopolsky MA

Sun Aug 01 00:00:00 UTC 2010

Men supplemented with 17beta-estradiol have increased beta-oxidation capacity in skeletal muscle.

Physiological genomics

Maher AC,Akhtar M,Tarnopolsky MA

Sun Aug 01 00:00:00 UTC 2010

Women have higher protein content of beta-oxidation enzymes in skeletal muscle than men.

PloS one

Maher AC,Akhtar M,Vockley J,Tarnopolsky MA

Fri Aug 06 00:00:00 UTC 2010

Women have higher protein content of beta-oxidation enzymes in skeletal muscle than men.

PloS one

Maher AC,Akhtar M,Vockley J,Tarnopolsky MA

Fri Aug 06 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: 3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit; 3-oxoacyl-CoA thiolase; 78 kDa gastrin-binding protein; gastrin-binding protein; hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit; long-chain 2-enoyl-CoA hydratase; long-chain-3-hydroxyacyl-CoA dehydrogenase; mitochondrial long-chain 2-enoyl-Coenzyme A (CoA) hydratase, alpha subunit; mitochondrial long-chain L-3-hydroxyacyl-Coenzyme A (CoA) dehydrogenase, alpha subunit; mitochondrial trifunctional enzyme, alpha subunit; mitochondrial trifunctional protein, alpha subunit; Monolysocardiolipin acyltransferase; TP-alpha; Trifunctional enzyme subunit alpha, mitochondrial

View more View less

Gene Aliases: C77020; ECHA; GBP; HADH; HADHA; LCEH; LCHAD; MTPA; RGD1560655; TP-ALPHA

View more View less

UniProt ID: (Human) P40939, (Rat) Q64428, (Mouse) Q8BMS1

View more View less

Entrez Gene ID: (Human) 3030, (Rat) 170670, (Mouse) 97212

View more View less

Function(s)

fatty-acyl-CoA binding 3-hydroxyacyl-CoA dehydrogenase activity acetyl-CoA C-acetyltransferase activity enoyl-CoA hydratase activity protein binding long-chain-enoyl-CoA hydratase activity long-chain-3-hydroxyacyl-CoA dehydrogenase activity protein complex binding NAD binding acetyl-CoA C-acyltransferase activity catalytic activity oxidoreductase activity lyase activity

Process(es)

fatty acid beta-oxidation response to insulin response to drug oxidation-reduction process cardiolipin acyl-chain remodeling lipid metabolic process fatty acid metabolic process metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

HADHA Polyclonal Antibody

HADHA Antibody (10758-1-AP) in ICC/IF

Product Details

10758-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!