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HADHA Monoclonal Antibody (3E9B1)

HADHA Antibody in Immunocytochemistry (ICC/IF)

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HADHA Antibody (60250-1-IG) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 60250-1-IG (HADHA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

HADHA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

60250-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3E9B1

Immunogen

HADHA Fusion Protein Ag1211 (339-763 aa encoded by BC009235)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LMGLYHGQV LCKKNKFGAP QKDVKHLAIL GAGLMGAGIA QVSVDKGLKT ILKDATLTAL DRGQQQVFKG LNDKVKKKAL TSFERDSIFS NLTGQLDYQG FEKADMVIEA VFEDLSLKHR VLKEVEAVIP DHCIFASNTS ALPISEIAAV SKRPEKVIGM HYFSPVDKMQ LLEIITTEKT SKDTSASAVA VGLKQGKVII VVKDGPGFYT TRCLAPMMSE VIRILQEGVD PKKLDSLTTS FGFPVGAATL VDEVGVDVAK HVAEDLGKVF GERFGGGNPE LLTQMVSKGF LGRKSGKGFY IYQEGVKRKD LNSDMDSILA SLKLPPKSEV SSDEDIQFRL VTRFVNEAVM CLQEGILATP AEGDIGAVFG LGFPPCLGGP FRFVDLYGAQ KIVDRLKKYE AAYGKQFTPC QLLADHANSP NKKFYQ (339-763 aa encoded by BC009235)

Target Information

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Spermidine activates mitochondrial trifunctional protein and improves antitumor immunity in mice.

Science (New York, N.Y.)

Al-Habsi M,Chamoto K,Matsumoto K,Nomura N,Zhang B,Sugiura Y,Sonomura K,Maharani A,Nakajima Y,Wu Y,Nomura Y,Menzies R,Tajima M,Kitaoka K,Haku Y,Delghandi S,Yurimoto K,Matsuda F,Iwata S,Ogura T,Fagarasan S,Honjo T

Fri Oct 28 00:00:00 UTC 2022

Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.

Frontiers in oncology

Lu J,Zhang Y,Sun M,Ding C,Zhang L,Kong Y,Cai M,Miccoli P,Ma C,Yue X

Fri Apr 29 00:00:00 UTC 2022

Glycolytic and lipid oxidative metabolic programs are essential for freshly-isolated regulatory T cells in mice with sepsis.

RSC advances

Zhu X,Ji W,Guo S,Zhu D,Yang Y,Liu X

Wed May 27 00:00:00 UTC 2020

Exosomes from hyperglycemia-stimulated vascular endothelial cells contain versican that regulate calcification/senescence in vascular smooth muscle cells.

Cell & bioscience

Li S,Zhan JK,Wang YJ,Lin X,Zhong JY,Wang Y,Tan P,He JY,Cui XJ,Chen YY,Huang W,Liu YS

Thu Mar 31 00:00:00 UTC 2022

Exosomes from hyperglycemia-stimulated vascular endothelial cells contain versican that regulate calcification/senescence in vascular smooth muscle cells.

Cell & bioscience

Li S,Zhan JK,Wang YJ,Lin X,Zhong JY,Wang Y,Tan P,He JY,Cui XJ,Chen YY,Huang W,Liu YS

Thu Mar 31 00:00:00 UTC 2022

Quantitative proteomic study of myocardial mitochondria in urea transporter B knockout mice.

Proteomics

Du Y,Meng Y,Zhu J,Kang L,Jia X,Guo L,Zhang L,Ye M,Hu L,Zhao X,Gu J,Yang B,Zou H

Mon Sep 01 00:00:00 UTC 2014

Quantitative proteomic study of myocardial mitochondria in urea transporter B knockout mice.

Proteomics

Du Y,Meng Y,Zhu J,Kang L,Jia X,Guo L,Zhang L,Ye M,Hu L,Zhao X,Gu J,Yang B,Zou H

Mon Sep 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: 3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit; 3-oxoacyl-CoA thiolase; 78 kDa gastrin-binding protein; gastrin-binding protein; hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit; long-chain 2-enoyl-CoA hydratase; long-chain-3-hydroxyacyl-CoA dehydrogenase; mitochondrial long-chain 2-enoyl-Coenzyme A (CoA) hydratase, alpha subunit; mitochondrial long-chain L-3-hydroxyacyl-Coenzyme A (CoA) dehydrogenase, alpha subunit; mitochondrial trifunctional enzyme, alpha subunit; mitochondrial trifunctional protein, alpha subunit; Monolysocardiolipin acyltransferase; TP-alpha; Trifunctional enzyme subunit alpha, mitochondrial

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Gene Aliases: C77020; ECHA; GBP; HADH; HADHA; LCEH; LCHAD; MTPA; TP-ALPHA

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UniProt ID: (Human) P40939, (Mouse) Q8BMS1

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Entrez Gene ID: (Human) 3030, (Mouse) 97212

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Function(s)

fatty-acyl-CoA binding 3-hydroxyacyl-CoA dehydrogenase activity acetyl-CoA C-acetyltransferase activity enoyl-CoA hydratase activity protein binding long-chain-enoyl-CoA hydratase activity long-chain-3-hydroxyacyl-CoA dehydrogenase activity protein complex binding NAD binding catalytic activity acetyl-CoA C-acyltransferase activity oxidoreductase activity lyase activity

Process(es)

fatty acid beta-oxidation response to insulin cardiolipin acyl-chain remodeling response to drug lipid metabolic process fatty acid metabolic process metabolic process oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HADHA Monoclonal Antibody (3E9B1)

HADHA Antibody (60250-1-IG) in ICC/IF

Product Details

60250-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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