Invitrogen

HAP1 Monoclonal Antibody (1B6)

4 Published Figures

4 References

View all (12) HAP1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (4)

HAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

HAP1 Antibody (MA1-46412) in IHC (P)

Immunohistochemical analysis of HAP1 in rat hypothalamus. Samples were incubated in HAP1 monoclonal antibody (Product # MA1-46412).

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Product Details

MA1-46412

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.0 µg/mL

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1.0 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

0.5-1.0 µg/mL

Immunocytochemistry (ICC/IF)

Assay-Dependent

Flow Cytometry (Flow)

1 µg per million cells

Immunoprecipitation (IP)

1:10-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1B6

Immunogen

Fusion protein from rat HAP1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 50% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2232841

Product Specific Information

In Western blot bands can be seen ~95 kDa for isoform A and ~110 kDa for isoform B.

Suggested positive control: rat brain.

Target Information

Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. HAP1 was initially identified through a two-hybrid library screening; the binding of HAP1 to huntingtin correlated with the expansion of the polyglutamine tract. HAP1 also interacts with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), suggesting that HAP1 may play a role in vesicular trafficking or organelle transport. HAP1 is also involved with the huntingtin-enhanced BDNF transport along the cellular microtubles. Attenuation of this process led to the loss of neurotrophic support and neuronal toxicity, which suggests that loss of this function might contribute to pathogenesis. Several alternatively spliced isoforms have been described for HAP1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathway.

The Journal of cell biology

Cason SE,Carman PJ,Van Duyne C,Goldsmith J,Dominguez R,Holzbaur ELF

MA1-46412 was used in Western Blot to identify multiple dynein effectors on autophagosomes as they transit along the axons of primary neurons.

Mon Jul 05 00:00:00 UTC 2021

Mutant huntingtin impairs PNKP and ATXN3, disrupting DNA repair and transcription.

eLife

Gao R,Chakraborty A,Geater C,Pradhan S,Gordon KL,Snowden J,Yuan S,Dickey AS,Choudhary S,Ashizawa T,Ellerby LM,La Spada AR,Thompson LM,Hazra TK,Sarkar PS

MA1-46412 was used in Western Blotting to suggest a potential molecular mechanism of how mutant HTT activates DNA damage-response pro-degenerative pathways and impairs transcription, triggering neurotoxicity and functional decline in HD.

Wed Apr 17 00:00:00 UTC 2019

Huntingtin-associated protein 1: Eutherian adaptation from a TRAK-like protein, conserved gene promoter elements, and localization in the human intestine.

BMC evolutionary biology

Lumsden AL,Young RL,Pezos N,Keating DJ

MA146412 was used in immunohistochemistry - paraffin section to discuss the evolution of huntingtin-associated Protein 1

Thu Oct 13 00:00:00 UTC 2016

Huntingtin associated protein 1 regulates trafficking of the amyloid precursor protein and modulates amyloid beta levels in neurons.

Journal of neurochemistry

Yang GZ,Yang M,Lim Y,Lu JJ,Wang TH,Qi JG,Zhong JH,Zhou XF

MA1-46412 was used in immunohistochemistry, immunoprecipitation, and western blot to study the regulation of APP trafficing and neuronal beta-amyloid levels by Huntingtin-associated protein-1

Sat Sep 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: HAP-1; HAP-1 antibody; Huntingtin-associated protein 1; huntingtin-associated protein 2; Neuroan 1

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Gene Aliases: HAP-1; HAP1; HAP1-A; HAP1-B; HAP2; hHLP1; HIP5; HLP; HLP1

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UniProt ID: (Human) P54257, (Rat) P54256, (Mouse) O35668

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Entrez Gene ID: (Human) 9001, (Rat) 29430, (Mouse) 15114

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Function(s)

protein binding ion channel binding brain-derived neurotrophic factor binding receptor binding protein domain specific binding identical protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HAP1 Monoclonal Antibody (1B6)

HAP1 Antibody (MA1-46412) in IHC (P)

Product Details

MA1-46412

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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