Proteintech

HAS3 Polyclonal Antibody

HAS3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HAS3 Antibody (15609-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15609-1-AP ( HAS3 Antibody) at dilution of 1:200 (under 40x lens).

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Product Details

15609-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Flow Cytometry (Flow)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HAS3 Fusion Protein Ag7999 (1-281 aa encoded by BC021853)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MPVQLTTAL RVVGTSLFAL AVLGGILAAY VTGYQFIHTE KHYLSFGLYG AILGLHLLIQ SLFAFLEHRR MRRAGQALKL PSPRRGSVAL CIAAYQEDPD YLRKCLRSAQ RISFPDLKVV MVVDGNRQED AYMLDIFHEV LGGTEQAGFF VWRSNFHEAG EGETEASLQE GMDRVRDVVR ASTFSCIMQK WGGKREVMYT AFKALGDSVD YIQVCDSDTV LDPACTIEML RVLEEDPQVG GVGGDVQPPG KGMAVEDDQV QAAQVRATEA WSVHQRHVSR EQ (1-281 aa encoded by BC021853)

Target Information

HAS1, HAS2 and HAS3 are HA (hyaluronan or hyaluronic acid) synthase proteins. The extracellular matrix in most vertebrates express HA, which is a high molecular weight linear polysaccharide composed of alternating glucuronic acid and N-acetylglucosamine residues linked by b-1,3 and b-1,4 glycosidic bonds. The three HAS genes show distinct patterns of expression during development and their protein products play significantly different roles in the formation of the HA matrix. Both HAS1 and HAS2 synthesize high molecular-weight HA, whereas HAS3 produces lower molecular weight HA. The expression of the three HAS isoforms is more prominent in growing cells than in resting cells and is differentially regulated by various stimuli, suggesting distinct functional roles of the three proteins. HAS3 produces both secreted and cell-associated forms of hyaluronan and is the most active of the three isoforms of this enzyme in adults. HAS3 gene expression plays a crucial role in the regulation of hyaluronan synthesis in the epidermis. Specifically, IFN-g markedly upregulates HAS3 mRNA, whereas TGFbeta downregulates HAS3 transcript levels. The human HAS3 gene maps to chromosome 16q22. 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A druggable copper-signalling pathway that drives inflammation.

Nature

Solier S,Müller S,Cañeque T,Versini A,Mansart A,Sindikubwabo F,Baron L,Emam L,Gestraud P,Pantoș GD,Gandon V,Gaillet C,Wu TD,Dingli F,Loew D,Baulande S,Durand S,Sencio V,Robil C,Trottein F,Péricat D,Näser E,Cougoule C,Meunier E,Bègue AL,Salmon H,Manel N,Puisieux A,Watson S,Dawson MA,Servant N,Kroemer G,Annane D,Rodriguez R

Mon May 01 00:00:00 UTC 2023

C1q-HA Matrix Regulates the Local Synthesis of Hyaluronan in Malignant Pleural Mesothelioma by Modulating HAS3 Expression.

Cancers

Vidergar R,Balduit A,Zacchi P,Agostinis C,Mangogna A,Belmonte B,Grandolfo M,Salton F,Biolo M,Zanconati F,Confalonieri M,Bulla R

Fri Jan 22 00:00:00 UTC 2021

C1q-HA Matrix Regulates the Local Synthesis of Hyaluronan in Malignant Pleural Mesothelioma by Modulating HAS3 Expression.

Cancers

Vidergar R,Balduit A,Zacchi P,Agostinis C,Mangogna A,Belmonte B,Grandolfo M,Salton F,Biolo M,Zanconati F,Confalonieri M,Bulla R

Fri Jan 22 00:00:00 UTC 2021

C1q-HA Matrix Regulates the Local Synthesis of Hyaluronan in Malignant Pleural Mesothelioma by Modulating HAS3 Expression.

Cancers

Vidergar R,Balduit A,Zacchi P,Agostinis C,Mangogna A,Belmonte B,Grandolfo M,Salton F,Biolo M,Zanconati F,Confalonieri M,Bulla R

Fri Jan 22 00:00:00 UTC 2021

Elevation of Hyaluronan Synthase by Magnesium Supplementation Mediated through the Activation of GSK3 and CREB in Human Keratinocyte-Derived HaCaT Cells.

International journal of molecular sciences

Marunaka K,Shu S,Kobayashi M,Goto M,Katsuta Y,Yoshino Y,Ikari A

Wed Dec 22 00:00:00 UTC 2021

Adenosine and hyaluronan promote lung fibrosis and pulmonary hypertension in combined pulmonary fibrosis and emphysema.

Disease models & mechanisms

Collum SD,Molina JG,Hanmandlu A,Bi W,Pedroza M,Mertens TCJ,Wareing N,Wei W,Wilson C,Sun W,Rajadas J,Bollyky PL,Philip KM,Ren D,Thandavarayan RA,Bruckner BA,Xia Y,Blackburn MR,Karmouty-Quintana H

Wed May 15 00:00:00 UTC 2019

Adenosine and hyaluronan promote lung fibrosis and pulmonary hypertension in combined pulmonary fibrosis and emphysema.

Disease models & mechanisms

Collum SD,Molina JG,Hanmandlu A,Bi W,Pedroza M,Mertens TCJ,Wareing N,Wei W,Wilson C,Sun W,Rajadas J,Bollyky PL,Philip KM,Ren D,Thandavarayan RA,Bruckner BA,Xia Y,Blackburn MR,Karmouty-Quintana H

Wed May 15 00:00:00 UTC 2019

miR‑29a‑3p represses proliferation and metastasis of gastric cancer cells via attenuating HAS3 levels.

Molecular medicine reports

Bai F,Jiu M,You Y,Feng Y,Xin R,Liu X,Mo L,Nie Y

Fri Jun 01 00:00:00 UTC 2018

miR‑29a‑3p represses proliferation and metastasis of gastric cancer cells via attenuating HAS3 levels.

Molecular medicine reports

Bai F,Jiu M,You Y,Feng Y,Xin R,Liu X,Mo L,Nie Y

Fri Jun 01 00:00:00 UTC 2018

Celecoxib and Pioglitazone as Potential Therapeutics for Regulating TGF-β-Induced Hyaluronan in Dysthyroid Myopathy.

Investigative ophthalmology & visual science

Cheng AM,Yin HY,Chen A,Liu YW,Chuang MC,He H,Tighe S,Sheha H,Liao SL

Fri Apr 01 00:00:00 UTC 2016

Celecoxib and Pioglitazone as Potential Therapeutics for Regulating TGF-β-Induced Hyaluronan in Dysthyroid Myopathy.

Investigative ophthalmology & visual science

Cheng AM,Yin HY,Chen A,Liu YW,Chuang MC,He H,Tighe S,Sheha H,Liao SL

Fri Apr 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: HA synthase 3; Hyaluronan synthase 3; Hyaluronate synthase 3; Hyaluronic acid synthase 3

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Gene Aliases: HAS3

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UniProt ID: (Human) O00219, (Mouse) O08650

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Entrez Gene ID: (Human) 3038, (Mouse) 15118, (Rat) 266805

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Function(s)

hyaluronan synthase activity transferase activity transferase activity, transferring glycosyl groups

Process(es)

cell adhesion hyaluronan biosynthetic process extracellular polysaccharide biosynthetic process positive regulation of transcription, DNA-templated extracellular matrix assembly positive regulation of hyaluranon cable assembly carbohydrate metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HAS3 Polyclonal Antibody

HAS3 Antibody (15609-1-AP) in IHC (P)

Product Details

15609-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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