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HAUS6 Antibody (PA5-92294) in WB
Western blot analysis of HAUS6 in extracts of various cell lines. Samples were incubated with HAUS6 Polyclonal antibody (Product # PA5-92294) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-92294
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 676-955 of human HAUS6 (NP_0601153)
Conjugate
Form
Liquid
Concentration
2.11 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806431
Product Specific Information
Immunogen sequence: DEPPTQNQSD LLNKKVICKQ DLECLAFTKL SETSRMETFS PAVGNRIDVM GGSEEEFMKI LDHLEVSCNK PSTNKTMLWN SFQISSGISS KSFKDNDFGI LHETLPEEVG HLSFNSSSSS EANFKLEPNS PMHGGTLLED VVGGRQTTPE SDFNLQALRS RYEALKKSLS KKREESYLSN SQTPERHKPE LSPTPQNVQT DDTLNFLDTC DLHTEHIKPS LRTSIGERKR SLSPLIKFSP VEQRLRTTIA CSLGELPNLK EEDILNKSLD AKEPPSDLTR; Positive Samples: Mouse spleen, Mouse thymus; Cellular Location: Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle
Target Information
HAUS6 is 1 of 8 subunits of the 390-kDa human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb augmentare, meaning to increase. The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 ; Uehara et al., 2009 ).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dim gamma-tubulin homolog; family with sequence similarity 29, member A; HAUS augmin-like complex subunit 6; HAUS augmin-like complex, subunit 6; likely ortholog of H. sapiens family with sequence similarity 29, member A (FAM29A)
Gene Aliases: 6230416J20Rik; 9830144E06; D4Ertd27e; DGT6; FAM29A; HAUS6; KIAA1574; mKIAA1574
UniProt ID: (Human) Q7Z4H7
Entrez Gene ID: (Human) 54801, (Mouse) 230376
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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