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HBP1 Polyclonal Antibody

HBP1 Antibody in Immunocytochemistry (ICC/IF)

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HBP1 Antibody (11746-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 11746-1-AP (HBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

HBP1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11746-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

ChIP assay (ChIP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HBP1 Fusion Protein Ag2342 (215-514 aa encoded by BC022329)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.09 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This is a rabbit polyclonal antibody raised against the C-terminus of human HBP1.

Immunogen sequence: FLKGTRLCF HKGSNKEWQD VEDFARAEGC DNEEDLQMGI HKGYGSDGLK LLSHEESVSF GESVLKLTFD PGTVEDGLLT VECKLDHPFY VKNKGWSSFY PSLTVVQHGI PCCEVHIGDV CLPPGHPDAI NFDDPGVFDT FKSYDFTPMD SSAVYVLSSM ARQRRASLSC GGPGGQDFAR SGFSKNCGSP GSSQLSSNSL YAKAVKNHSS GTVSATSPNK CKRPMNAFML FAKKYRVEYT QMYPGKDNRA ISVILGDRWK KMKNEERRMY TLEAKALAEE QKRLNPDCWK RKRTNSGSQQ H (215-514 aa encoded by BC022329)

Target Information

The HMG-box protein-1 (HBP1) is a member of the HMG family of transcription factors, which are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, that mediates DNA binding. HBP1 binds to the tumor suppressor proteins Rb and p130 and initiates cell cycle arrest. Terminal cell differentiation requires this initial cell cycle arrest followed by the coordinated expression of genes defined as tissue-specific markers. Along with initiating the commitment to cell differentiation, the continued activity of HBP1 abrogates the expression of tissue-specific genes by associating with the MyoD proteins. In muscle cell differentiation, the MyoD family of transcription factors, which include Myf5, MyoD and myogenein, induce the expression of these cell-type specific proteins and contribute to the development of cell phenotypes. The progression of terminal differentiation is, therefore, dependent on both a decrease in HBP1 activity and the corresponding activation of MyoD-induced gene transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HBP1 inhibits the development of type 2 diabetes mellitus through transcriptional activation of the IGFBP1 gene.

Aging

Cheng Y,Yang R,Zhou Y,Wang J,Zhang T,Wang S,Li H,Jiang W,Zhang X

Wed Nov 02 00:00:00 UTC 2022

CRISPR interference and activation of the microRNA-3662-HBP1 axis control progression of triple-negative breast cancer.

Oncogene

Yi B,Wang S,Wang X,Liu Z,Zhang C,Li M,Gao S,Wei S,Bae S,Stringer-Reasor E,Wang L,Liu R

Sat Jan 01 00:00:00 UTC 2022

CRISPR interference and activation of the microRNA-3662-HBP1 axis control progression of triple-negative breast cancer.

Oncogene

Yi B,Wang S,Wang X,Liu Z,Zhang C,Li M,Gao S,Wei S,Bae S,Stringer-Reasor E,Wang L,Liu R

Sat Jan 01 00:00:00 UTC 2022

The human GID complex engages two independent modules for substrate recruitment.

EMBO reports

Mohamed WI,Park SL,Rabl J,Leitner A,Boehringer D,Peter M

Thu Nov 04 00:00:00 UTC 2021

miR-29c&b2 encourage extramedullary infiltration resulting in the poor prognosis of acute myeloid leukemia.

Oncogene

Wei Y,Lu W,Yu Y,Zhai Y,Guo H,Yang S,Zhao C,Zhang Y,Liu J,Liu Y,Fei J,Shi J

Sat May 01 00:00:00 UTC 2021

miR-29c&b2 encourage extramedullary infiltration resulting in the poor prognosis of acute myeloid leukemia.

Oncogene

Wei Y,Lu W,Yu Y,Zhai Y,Guo H,Yang S,Zhao C,Zhang Y,Liu J,Liu Y,Fei J,Shi J

Sat May 01 00:00:00 UTC 2021

miR-29c&b2 encourage extramedullary infiltration resulting in the poor prognosis of acute myeloid leukemia.

Oncogene

Wei Y,Lu W,Yu Y,Zhai Y,Guo H,Yang S,Zhao C,Zhang Y,Liu J,Liu Y,Fei J,Shi J

Sat May 01 00:00:00 UTC 2021

miR-29c&b2 encourage extramedullary infiltration resulting in the poor prognosis of acute myeloid leukemia.

Oncogene

Wei Y,Lu W,Yu Y,Zhai Y,Guo H,Yang S,Zhao C,Zhang Y,Liu J,Liu Y,Fei J,Shi J

Sat May 01 00:00:00 UTC 2021

HBP1-mediated transcriptional repression of AFP inhibits hepatoma progression.

Journal of experimental & clinical cancer research : CR

Cao Z,Cheng Y,Wang J,Liu Y,Yang R,Jiang W,Li H,Zhang X

Thu Apr 01 00:00:00 UTC 2021

MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1.

Oncogene

Cao Z,Xue J,Cheng Y,Wang J,Liu Y,Li H,Jiang W,Li G,Gui Y,Zhang X

Sat Jun 01 00:00:00 UTC 2019

MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1.

Oncogene

Cao Z,Xue J,Cheng Y,Wang J,Liu Y,Li H,Jiang W,Li G,Gui Y,Zhang X

Sat Jun 01 00:00:00 UTC 2019

Transcription factor HMG box-containing protein 1 (HBP1) modulates mitotic clonal expansion (MCE) during adipocyte differentiation.

Journal of cellular physiology

Chan CY,Yu P,Chang FT,Chen ZH,Lee MF,Huang CY

Tue May 01 00:00:00 UTC 2018

A positive feedback loop between Pim-1 kinase and HBP1 transcription factor contributes to hydrogen peroxide-induced premature senescence and apoptosis.

The Journal of biological chemistry

Wang S,Cao Z,Xue J,Li H,Jiang W,Cheng Y,Li G,Zhang X

Fri May 19 00:00:00 UTC 2017

A positive feedback loop between Pim-1 kinase and HBP1 transcription factor contributes to hydrogen peroxide-induced premature senescence and apoptosis.

The Journal of biological chemistry

Wang S,Cao Z,Xue J,Li H,Jiang W,Cheng Y,Li G,Zhang X

Fri May 19 00:00:00 UTC 2017

HBP1-mediated Regulation of p21 Protein through the Mdm2/p53 and TCF4/EZH2 Pathways and Its Impact on Cell Senescence and Tumorigenesis.

The Journal of biological chemistry

Chen Y,Pan K,Wang P,Cao Z,Wang W,Wang S,Hu N,Xue J,Li H,Jiang W,Li G,Zhang X

Fri Jun 10 00:00:00 UTC 2016

HBP1-mediated Regulation of p21 Protein through the Mdm2/p53 and TCF4/EZH2 Pathways and Its Impact on Cell Senescence and Tumorigenesis.

The Journal of biological chemistry

Chen Y,Pan K,Wang P,Cao Z,Wang W,Wang S,Hu N,Xue J,Li H,Jiang W,Li G,Zhang X

Fri Jun 10 00:00:00 UTC 2016

MYC through miR-17-92 suppresses specific target genes to maintain survival, autonomous proliferation, and a neoplastic state.

Cancer cell

Li Y,Choi PS,Casey SC,Dill DL,Felsher DW

Mon Aug 11 00:00:00 UTC 2014

MYC through miR-17-92 suppresses specific target genes to maintain survival, autonomous proliferation, and a neoplastic state.

Cancer cell

Li Y,Choi PS,Casey SC,Dill DL,Felsher DW

Mon Aug 11 00:00:00 UTC 2014

HBP1-mediated transcriptional regulation of DNA methyltransferase 1 and its impact on cell senescence.

Molecular and cellular biology

Pan K,Chen Y,Roth M,Wang W,Wang S,Yee AS,Zhang X

Fri Mar 01 00:00:00 UTC 2013

HBP1-mediated transcriptional regulation of DNA methyltransferase 1 and its impact on cell senescence.

Molecular and cellular biology

Pan K,Chen Y,Roth M,Wang W,Wang S,Yee AS,Zhang X

Fri Mar 01 00:00:00 UTC 2013

HBP1-mediated transcriptional regulation of DNA methyltransferase 1 and its impact on cell senescence.

Molecular and cellular biology

Pan K,Chen Y,Roth M,Wang W,Wang S,Yee AS,Zhang X

Fri Mar 01 00:00:00 UTC 2013

HBP1-mediated transcriptional regulation of DNA methyltransferase 1 and its impact on cell senescence.

Molecular and cellular biology

Pan K,Chen Y,Roth M,Wang W,Wang S,Yee AS,Zhang X

Fri Mar 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: High mobility group box transcription factor 1; HMG box transcription factor 1; HMG box-containing protein 1

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Gene Aliases: 1700058O05Rik; BE629963; C330012F01Rik; C86454; HBP1

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UniProt ID: (Human) O60381, (Mouse) Q8R316

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Entrez Gene ID: (Human) 26959, (Mouse) 73389

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Function(s)

DNA binding RNA binding protein binding

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated cell cycle arrest Wnt signaling pathway positive regulation of potassium ion transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HBP1 Polyclonal Antibody

HBP1 Antibody (11746-1-AP) in ICC/IF

Product Details

11746-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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