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HCAR2/HCAR3 Antibody (PA5-99937) in ICC/IF
Immunocytochemistry analysis of HCAR2/HCAR3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with HCAR2/HCAR3 polyclonal antibody (Product # PA5-99937) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugate... View More
Product Details
PA5-99937
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HCAR2(Accession Q8TDS4), corresponding to amino acid residues F304-S354.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2818870
Product Specific Information
Antibody detects endogenous levels of total GPR109A/B.
Target Information
Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein-coupled receptor 109A; G protein-coupled receptor 109B; G protein-coupled receptor HM74a; G-protein coupled receptor 109A; G-protein coupled receptor 109B; G-protein coupled receptor HM74; G-protein coupled receptor HM74A; G-protein coupled receptor HM74B; GTP-binding protein; hydroxy-carboxylic acid receptor 2; hydroxy-carboxylic acid receptor 3; Hydroxycarboxylic acid receptor 2; Hydroxycarboxylic acid receptor 3; Niacin receptor 1; Niacin receptor 2; Nicotinic acid receptor; Nicotinic acid receptor 2; putative chemokine receptor
Gene Aliases: GPR109A; GPR109B; HCA2; HCA3; HCAR2; HCAR3; HM74; HM74A; HM74B; NIACR1; NIACR2; Puma-g; PUMAG
UniProt ID: (Human) Q8TDS4, (Human) P49019
Entrez Gene ID: (Human) 338442, (Human) 8843
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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