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Invitrogen
HCFC1 Polyclonal Antibody
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HCFC1 Antibody (PA5-30222) in WB
Western Blot analysis of HCFC1 was performed by separating 30 µg of A549 whole cell and nuclear extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HCFC1 Polyclonal Antibody (Product # PA5-30222) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-30222
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human HCF1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.76 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547696
Product Specific Information
Recommended positive controls: HeLa, A549, A549 nuclear extract, rat brain.
Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Tumor necrosis factor (TNF) receptor associated factors (TRAFs) were initially discovered as adaptor proteins that link the TNF receptor superfamily to signaling pathways and are thus important regulators of cell death and cellular response to stress. TRAF proteins share a homology region that allows them to bind to cell receptors and other TRAF proteins, causing the activation of different signal cascades depending on the TRAFs involved. For example, TRAF2 and TRAF3 directly bind to the CD40, a NF receptor superfamily member involved in inducing B cell immunity, and are critical for NF-kappa-B activation in mouse B lymphocytes. TRAF2 along with TRAF6 has also been shown to be required for CD40 signaling in nonhemopoietic cells. TRAF2 also interacts with the TRFR superfamily member lymphotoxin-beta receptor (LTbetaR) in association with TRAF3 and the apoptosis inhibitors cIAP1 and Smac.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C1 factor; CFF; HCF; Host cell factor 1; protein phosphatase 1, regulatory subunit 89; VCAF; VP16 accessory protein; VP16-accessory protein
Gene Aliases: CFF; HCF; HCF-1; HCF1; HCFC1; HFC1; MRX3; PPP1R89; RGD1563804; VCAF
UniProt ID: (Human) P51610
Entrez Gene ID: (Human) 3054, (Rat) 363519
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
identical protein binding
histone acetyltransferase activity (H4-K5 specific)
histone acetyltransferase activity (H4-K8 specific)
histone acetyltransferase activity (H4-K16 specific)
cadherin binding involved in cell-cell adhesion
molecular_function
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
positive regulation of gene expression
release from viral latency
regulation of protein complex assembly
histone H4-K5 acetylation
histone H4-K8 acetylation
histone H4-K16 acetylation
positive regulation of transcription from RNA polymerase II promoter
protein stabilization
cellular response to organic cyclic compound
mitochondrion organization
cell cycle
positive regulation of cell cycle
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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