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Invitrogen
HCN1 Polyclonal Antibody
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FIGURE: 1 / 11
HCN1 Antibody (PA5-78675) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of HCN1 was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HCN1 Polyclonal Antibody (Product # PA5-78675) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Product Details
PA5-78675
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the Intracellular domain of human HCN1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.45 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735891
Product Specific Information
Positive Control: SK-N-AS, mouse brain, rat brain
Predicted Reactivity: Rabbit (90%), Bovine (89%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HCN1 includes Hyperpolarization-activated cation channels of the HCN gene family, which contribute to spontaneous rhythmic activity in both heart and brain. HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. The HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BCNG-1; Brain cyclic nucleotide-gated channel 1; HAC-2; Hyperpolarization-activated cation channel 2; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Gene Aliases: BCNG-1; BCNG1; C630013B14Rik; EIEE24; HAC-2; HAC2; HCN1
UniProt ID: (Human) O60741, (Mouse) O88704, (Rat) Q9JKB0
Entrez Gene ID: (Human) 348980, (Mouse) 15165, (Rat) 84390
intracellular cAMP activated cation channel activity
voltage-gated sodium channel activity
voltage-gated potassium channel activity
potassium channel activity
cAMP binding
identical protein binding
nucleotide binding
ion channel activity
voltage-gated ion channel activity
sodium channel activity
protein binding
phosphatidylinositol-4,5-bisphosphate binding
phosphatidylinositol-3,4,5-trisphosphate binding
protein C-terminus binding
protein complex binding
regulation of membrane depolarization
transport
ion transport
potassium ion transport
sodium ion transport
regulation of ion transmembrane transport
sodium ion transmembrane transport
regulation of membrane potential
apical protein localization
negative regulation of action potential
retinal cone cell development
response to calcium ion
transmembrane transport
cellular response to cAMP
potassium ion transmembrane transport
regulation of membrane hyperpolarization
positive regulation of cation channel activity
inflammatory response
aging
cellular response to interferon-beta
maternal behavior
response to cisplatin
response to L-glutamate
regulation of postsynaptic membrane potential
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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