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Invitrogen
HCN1 Monoclonal Antibody (RTQ 7C3), eBioscience™
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FIGURE: 1 / 2
HCN1 Antibody (14-5691-82) in WB
Western Blot analysis was performed on whole cell extracts of Rat Brain (Lane 1), Mouse Brain (Lane 2) and HEK-293T(Lane 3) cells. The proteins were separated on a 4-12% Nupage Bis-Tris Gel (Product # NP0336BOX) using MOPS SDS Running Buffer (Product # NP0001-02). Protein transfer to a PVDF membrane (Product # IB24002) was achieved using the iBlot 2 system. Subsequently, the membrane was blocked with 5% milk and probed overnight at 4 C with 1.0 µg/mL Anti-Mouse/Rat HCN1 Monoclonal Antibody (clone RTQ-7C3), followed by Goat anti-Rat IgG Sec... View More
Product Details
14-5691-82
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
RTQ 7C3
Immunogen
aa 650 - 685 of rat HCN1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802194
Product Specific Information
Description: This RTQ 7C3 monoclonal antibody recognizes mouse and rat HCN1. Clone RTQ 7C3 detects a ~110 kDa band corresponding to HCN1 by Western Blot.
Applications Reported: This RTQ 7C3 antibody has been reported for use in western blot.
Applications Tested: This RTQ 7C3 antibody has been tested by western blot of mouse and rat tissue lysates. This may be used at less than or equal to 1 µg/mL.
Target Information
HCN1 includes Hyperpolarization-activated cation channels of the HCN gene family, which contribute to spontaneous rhythmic activity in both heart and brain. HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. The HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BCNG-1; Brain cyclic nucleotide-gated channel 1; HAC-2; Hyperpolarization-activated cation channel 2; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Gene Aliases: Bcng1; C630013B14Rik; HAC2; Hcn1
UniProt ID: (Mouse) O88704, (Rat) Q9JKB0
Entrez Gene ID: (Mouse) 15165, (Rat) 84390
nucleotide binding
ion channel activity
intracellular cAMP activated cation channel activity
voltage-gated ion channel activity
voltage-gated sodium channel activity
voltage-gated potassium channel activity
potassium channel activity
sodium channel activity
protein binding
phosphatidylinositol-4,5-bisphosphate binding
phosphatidylinositol-3,4,5-trisphosphate binding
protein C-terminus binding
cAMP binding
protein complex binding
identical protein binding
regulation of membrane depolarization
transport
ion transport
potassium ion transport
sodium ion transport
regulation of ion transmembrane transport
sodium ion transmembrane transport
regulation of membrane potential
apical protein localization
negative regulation of action potential
retinal cone cell development
response to calcium ion
transmembrane transport
cellular response to cAMP
potassium ion transmembrane transport
regulation of membrane hyperpolarization
positive regulation of cation channel activity
inflammatory response
aging
cellular response to interferon-beta
maternal behavior
response to cisplatin
response to L-glutamate
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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