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Invitrogen
HCN2 Monoclonal Antibody (QQA 4A6), eBioscience™
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FIGURE: 1 / 3
HCN2 Antibody (14-9692-82) in IHC (F)
Immunohistochemical analysis of HCN2 in a mouse frozen coronal brain section. Sample was incubated with HCN2 monoclonal antibody QQA 4A6 (40 ug/mL) for 1 hour at RT and followed by Goat Anti-Rat HRP and visualized with DAB Chromogen. Nuclei were counterstained with Mayer’s Hematoxylin.
Product Details
14-9692-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
QQA 4A6
Immunogen
Peptide corresponding to aa676-710
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2811746
Product Specific Information
Description: This QQA 4A6 monoclonal antibody recognizes mouse and rat HCN2. QQA 4A6 detects a ~95 kDa band corresponding to HCN2 by Western Blot. An off target band of ~80 kDa is detected in cell line models.
Applications Reported: This QQA 4A6 antibody has been reported for use in western blot and immunohistochemical staining.
Applications Tested: This QQA 4A6 antibody has been tested by western blot and may be used at less than or equal to 1.0 µg/mL The QQA 4A6 antibody has been tested by immunohistochemistry of frozen mouse tissue sections and can be used at less than or equal to 40 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Hyperpolarization-activated cation channels of the HCN gene family such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain. HCN2 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells; as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. The HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BCNG-2; Brain cyclic nucleotide-gated channel 2; HAC-1; Hyperpolarization-activated cation channel 1; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Gene Aliases: BCNG2; HAC1; Hcn2; trls
UniProt ID: (Mouse) O88703, (Rat) Q9JKA9
Entrez Gene ID: (Mouse) 15166, (Rat) 114244
nucleotide binding
ion channel activity
intracellular cAMP activated cation channel activity
voltage-gated ion channel activity
voltage-gated sodium channel activity
voltage-gated potassium channel activity
potassium channel activity
sodium channel activity
protein binding
PDZ domain binding
cAMP binding
protein complex binding
protein complex scaffold
identical protein binding
transport
ion transport
potassium ion transport
sodium ion transport
regulation of ion transmembrane transport
sodium ion transmembrane transport
regulation of membrane potential
positive regulation of heart contraction
transmembrane transport
cellular response to cAMP
cellular response to cGMP
potassium ion transmembrane transport
inflammatory response
brain development
aging
response to hormone
response to drug
cellular response to steroid hormone stimulus
response to cisplatin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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