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Invitrogen
HCN4 Monoclonal Antibody (SHG 1E5)
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HCN4 Antibody (MA3-903) in IHC (P)
Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rat monoclonal antibody r... View More
Product Details
MA3-903
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rat
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
SHG 1E5
Immunogen
Synthetic peptide sequence corresponding to residues S H G S L L L P P A S S P P P P Q V P Q R R G T P P L T P G R L T Q D L K L of HCN4.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2120037
Product Specific Information
MA3-903 detects HCN4 from human, mouse, and rat samples.
MA3-903 has been successfully used in Western blotting and immunohistochemistry procedures. By Western blot MA3-903 detects a ~132 KDa band representing HCN4.
The MA3-903 immunogen is a synthetic peptide sequence corresponding to residues S H G S L L L P P A S S P P P P Q V P Q R R G T P P L T P G R L T Q D L K L of HCN4.
MA3-903 clone SHG 1E5 is a rat monoclonal hybridoma. This antibody was produced by immunizing a rat, isolating the spleen cells, creating the hybridoma and successively injecting these cells into a mouse to produce ascites.
Target Information
HCN4 is a member of the family of hyperpolarization-activated and cyclic-nucleotide-gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BCNG-3; Brain cyclic nucleotide-gated channel 3; hyperpolarization activated cyclic nucleotide-gated cation channel 4; hyperpolarization activated cyclic nucleotide-gated potassium channel 4; hyperpolarization-activated, cyclic nucleotide-gated K+ 3; hyperpolarization-activated, cyclic nucleotide-gated K+ 4; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Gene Aliases: Bcng3; Hcn3; HCN4; SSS2
UniProt ID: (Human) Q9Y3Q4, (Mouse) O70507, (Rat) Q9JKA7
Entrez Gene ID: (Human) 10021, (Mouse) 330953, (Rat) 59266
intracellular cAMP activated cation channel activity
voltage-gated sodium channel activity
voltage-gated potassium channel activity
cAMP binding
identical protein binding
nucleotide binding
ion channel activity
voltage-gated ion channel activity
potassium channel activity
sodium channel activity
protein binding
in utero embryonic development
regulation of heart rate
regulation of membrane depolarization
transport
ion transport
cation transport
potassium ion transport
sodium ion transport
regulation of heart contraction
regulation of ion transmembrane transport
sodium ion transmembrane transport
regulation of membrane potential
transmembrane transport
regulation of cardiac muscle contraction
cellular response to cAMP
cellular response to cGMP
potassium ion transmembrane transport
SA node cell action potential
regulation of heart rate by cardiac conduction
cation transmembrane transport
muscle contraction
blood circulation
regulation of postsynaptic membrane potential
modulation of synaptic transmission
cellular response to steroid hormone stimulus
response to cisplatin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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