Rockland

HDAC1 (C-terminus) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

600-401-879

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-

ELISA (ELISA)

1:10,000-1:50,000
-

Immunoprecipitation (IP)

1:1,000-1:5,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 450-482 of Human HDAC-1.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.33 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-HDAC-1 antibody is directed against human HDAC-1 protein. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and chimpanzee sources based on 100% homology for the immunogen sequence. Cross reactivity may occur with HDAC-1 from bovine (82% homology) and chicken (80% homology) sources. Cross reactivity with HDAC-1 homologues from other sources has not been determined.

Target Information

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp686H12203; HD1; Histone deacetylase 1; Protein deacetylase HDAC1; Protein deacylase HDAC1; reduced potassium dependency, yeast homolog-like 1; RP4-811H24.2

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Gene Aliases: GON-10; HD1; HDAC1; RPD3; RPD3L1

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UniProt ID: (Human) Q13547

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Entrez Gene ID: (Human) 3065

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding core promoter binding RNA polymerase II transcription factor binding RNA polymerase II repressing transcription factor binding RNA polymerase II transcription corepressor activity transcription factor activity, sequence-specific DNA binding histone deacetylase activity protein binding transcription factor binding deacetylase activity enzyme binding nucleosomal DNA binding NAD-dependent histone deacetylase activity (H3-K14 specific) protein deacetylase activity activating transcription factor binding histone deacetylase binding transcription regulatory region DNA binding protein N-terminus binding NF-kappaB binding repressing transcription factor binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter chromatin remodeling methylation-dependent chromatin silencing transcription, DNA-templated protein deacetylation blood coagulation positive regulation of cell proliferation epidermal cell differentiation negative regulation of gene expression negative regulation of myotube differentiation positive regulation of receptor biosynthetic process viral process chromatin modification histone deacetylation circadian regulation of gene expression odontogenesis of dentin-containing tooth embryonic digit morphogenesis ATP-dependent chromatin remodeling negative regulation of apoptotic process negative regulation by host of viral transcription negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of androgen receptor signaling pathway hair follicle placode formation eyelid development in camera-type eye fungiform papilla formation histone H3 deacetylation histone H4 deacetylation negative regulation of canonical Wnt signaling pathway regulation of signal transduction by p53 class mediator beta-catenin-TCF complex assembly
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