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          • Primary Antibodies ›
          • HDAC2 Antibodies

          Proteintech

          HDAC2 Polyclonal Antibody

          76 References
          View all (41) HDAC2 antibodies

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          Cite HDAC2 Polyclonal Antibody

          • Antibody Testing Data (19)
          HDAC2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          HDAC2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 19

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          HDAC2 Antibody (12922-3-AP) in ICC/IF

          Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC2 antibody (Product # 12922-3-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          HDAC2 Antibody in Immunocytochemistry (ICC/IF)
          HDAC2 Antibody in Immunocytochemistry (ICC/IF)
          HDAC2 Antibody in Immunocytochemistry (ICC/IF)
          HDAC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HDAC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HDAC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HDAC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HDAC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HDAC2 Antibody in Western Blot (WB)
          HDAC2 Antibody in Western Blot (WB)
          HDAC2 Antibody in Western Blot (WB)
          HDAC2 Antibody in Western Blot (WB)
          HDAC2 Antibody in Western Blot (WB)
          HDAC2 Antibody in Western Blot (WB)
          HDAC2 Antibody in Western Blot (WB)
          HDAC2 Antibody in Western Blot (WB)
          HDAC2 Antibody in Western Blot (WB)
          HDAC2 Antibody in Flow Cytometry (Flow)
          HDAC2 Antibody in Immunoprecipitation (IP)

          Product Details

          12922-3-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 50 publications 50 publications

          Immunohistochemistry (IHC)

          -
          View 12 publications 12 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:10-1:100
          View 5 publications 5 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          -

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 6 publications 6 publications

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication

          Not applicable (N/A)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          HDAC2 Fusion Protein Ag3607 (274-571 aa encoded by BC031055)

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.29 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.

          Immunogen sequence: TDRVMTVSF HKYGEYFPGT GDLRDIGAGK GKYYAVNFPM RDGIDDESYG QIFKPIISKV MEMYQPSAVV LQCGADSLSG DRLGCFNLTV KGHAKCVEVV KTFNLPLLML GGGGYTIRNV ARCWTHETAV ALDCEIPNEL PYNDYFEYFG PDFKLHISPS NMTNQNTPEY MEKIKQRLFE NLRMLPHAPG VQMQAIPEDA VHEDSGDEDG EDPDKRISIR ASDKRIACDE EFSDSEDEGE GGRRNVADHK KGAKKARIEE DKKETEDKKT DVKEEDKSKD NSGEKTDTKG TKSEQLSNP (274-571 aa encoded by BC031055)

          Target Information

          HDAC2 belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: HD2; hdac 2; hdac-2; Histone deacetylase 2; Protein deacylase HDAC2; transcriptional regulator homolog RPD3; YY1 transcription factor-binding protein; YY1-associated factor 1

          View more View less

          Gene Aliases: D10Wsu179e; HD2; HDAC2; mRPD3; RPD3; YAF1; Yy1bp

          View more View less

          UniProt ID: (Human) Q92769, (Mouse) P70288

          View more View less

          Entrez Gene ID: (Human) 3066, (Mouse) 15182, (Rat) 84577

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding core promoter binding RNA polymerase II repressing transcription factor binding chromatin binding histone deacetylase activity protein binding transcription factor binding deacetylase activity enzyme binding heat shock protein binding nucleosomal DNA binding NAD-dependent histone deacetylase activity (H3-K14 specific) protein deacetylase activity sequence-specific DNA binding poly(A) RNA binding NF-kappaB binding transcription factor activity, sequence-specific DNA binding hydrolase activity histone deacetylase activity (H3-K14 specific) chromatin DNA binding histone deacetylase activity (H3-K9 specific) histone deacetylase activity (H4-K16 specific) Krueppel-associated box domain binding NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K18 specific)
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter response to amphetamine cardiac muscle hypertrophy chromatin remodeling maintenance of chromatin silencing transcription, DNA-templated blood coagulation positive regulation of cell proliferation epidermal cell differentiation positive regulation of epithelial to mesenchymal transition positive regulation of receptor biosynthetic process negative regulation of neuron projection development dendrite development histone deacetylation response to caffeine response to lipopolysaccharide positive regulation of interleukin-1 production positive regulation of tumor necrosis factor production circadian regulation of gene expression positive regulation of collagen biosynthetic process cellular response to heat response to nicotine response to cocaine odontogenesis of dentin-containing tooth response to drug positive regulation of tyrosine phosphorylation of Stat3 protein embryonic digit morphogenesis ATP-dependent chromatin remodeling negative regulation of apoptotic process negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of MHC class II biosynthetic process positive regulation of proteolysis negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter behavioral response to ethanol positive regulation of oligodendrocyte differentiation response to hyperoxia hair follicle placode formation negative regulation of dendritic spine development eyelid development in camera-type eye fungiform papilla formation cellular response to hydrogen peroxide histone H3 deacetylation histone H4 deacetylation cellular response to retinoic acid cellular response to transforming growth factor beta stimulus regulation of signal transduction by p53 class mediator cellular response to dopamine negative regulation of peptidyl-lysine acetylation regulation of transcription, DNA-templated protein deacetylation chromatin modification hippocampus development neuron differentiation rhythmic process positive regulation of sequence-specific DNA binding transcription factor activity regulation of protein kinase B signaling cardiac muscle cell development negative regulation of cardiac muscle cell proliferation regulation of sarcomere organization negative regulation of canonical Wnt signaling pathway regulation of protein deacetylation negative regulation of intrinsic apoptotic signaling pathway histone H3-K9 modification
          It has to be done as per old AB suggested Products section.
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