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HDAC4 (internal) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

600-401-J20

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (IHC)

1 mg/mL
-

ELISA (ELISA)

1:20,000-1:60,000
-

Immunoprecipitation (IP)

1 mg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HDAC4 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 600-650aa of human HDAC4.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific towards HDAC4. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, and Primate based on 100% sequence homology. Cross-reactivity with HDAC4 from other sources has not been determined.

Target Information

Histone deacetylase 4 (HDAC4) is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is also involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. It is also involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Mutations to the HDAC4 gene have been found in Brachydactyly-mental retardation syndrome (BDMR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp779K1053; HD4; HD7; HD7b; HD9; HDAC; HDAC7; HDAC7B; HDAC9; HDAC9B; HDAC9FL; HDRP; Histone deacetylase 4; histone deacetylase A; KIAA0744; MITR

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Gene Aliases: 4932408F19Rik; AHO3; AI047285; BDMR; HA6116; HD4; HDAC-4; HDAC-A; HDAC4; HDACA; KIAA0288

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UniProt ID: (Human) P56524, (Rat) Q99P99, (Mouse) Q6NZM9

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Entrez Gene ID: (Human) 9759, (Rat) 363287, (Mouse) 208727

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Function(s)
RNA polymerase III transcription factor binding core promoter binding chromatin binding transcription corepressor activity histone deacetylase activity protein binding transcription factor binding zinc ion binding protein kinase binding potassium ion binding NAD-dependent histone deacetylase activity (H3-K14 specific) protein deacetylase activity activating transcription factor binding histone deacetylase binding sequence-specific DNA binding transcription regulatory region DNA binding repressing transcription factor binding molecular_function DNA binding NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K18 specific) hydrolase activity histone deacetylase activity (H3-K14 specific) histone deacetylase activity (H3-K9 specific) histone deacetylase activity (H4-K16 specific) metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter skeletal system development osteoblast development chromatin remodeling transcription, DNA-templated inflammatory response nervous system development positive regulation of cell proliferation negative regulation of cell proliferation positive regulation of lamellipodium assembly negative regulation of myotube differentiation regulation of cardiac muscle contraction by calcium ion signaling response to denervation involved in regulation of muscle adaptation cardiac muscle hypertrophy in response to stress positive regulation of smooth muscle cell migration chromatin modification histone deacetylation B cell differentiation positive regulation of protein sumoylation peptidyl-lysine deacetylation regulation of gene expression, epigenetic B cell activation response to drug regulation of protein binding negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of neuron apoptotic process negative regulation of osteoblast differentiation negative regulation of glycolytic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle cell proliferation regulation of skeletal muscle fiber development positive regulation of sequence-specific DNA binding transcription factor activity response to interleukin-1 histone H3 deacetylation histone H4 deacetylation cellular response to mechanical stimulus cellular response to tumor necrosis factor cellular response to parathyroid hormone stimulus positive regulation of reactive oxygen species biosynthetic process protein deacetylation regulation of striated muscle cell differentiation histone H3-K9 modification regulation of transcription, DNA-templated
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