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Invitrogen
HDAC9 Monoclonal Antibody (OTI7G2)
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FIGURE: 1 / 23
HDAC9 Antibody (MA5-26729) in ICC/IF
Immunofluorescence analysis of HDAC9 was performed using U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HDAC9 Monoclonal Antibody (Product # MA5-26729) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A32723) at a dilution of 1:2000 for 45 minutes at... View More
Product Details
MA5-26729
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI7G2
Immunogen
Human recombinant protein fragment corresponding to amino acids 181-460 of HDAC9 produced in E.coli
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2724428
Target Information
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical component of transcriptional regulation and the acetylation of nucleosomal histones is a major source of this remodeling. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1-9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity and repress myoctye enhancer-binding factor 2-mediated transcription. HDAC9 and HDAC9a are expressed in brain, skeletal muscle, kidney, placenta and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp779K1053; HD7; HD9; hdac-9; histone deacetylase 4/5-related protein; Histone deacetylase 7B; Histone deacetylase 9; Histone deacetylase-related protein; MEF-2 interacting transcription repressor (MITR) protein; MEF2-interacting transcription repressor MITR
Gene Aliases: HD7; HD7b; HD9; HDAC; HDAC7; HDAC7B; HDAC9; HDAC9B; HDAC9FL; HDRP; KIAA0744; MITR
UniProt ID: (Human) Q9UKV0
Entrez Gene ID: (Human) 9734
transcription corepressor activity
histone deacetylase activity
protein kinase C binding
protein binding
transcription factor binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
protein deacetylase activity
histone deacetylase binding
metal ion binding
repressing transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
response to amphetamine
transcription, DNA-templated
inflammatory response
heart development
chromatin modification
histone deacetylation
neuron differentiation
B cell differentiation
cellular response to insulin stimulus
peptidyl-lysine deacetylation
B cell activation
negative regulation of transcription, DNA-templated
regulation of skeletal muscle fiber development
regulation of striated muscle cell differentiation
histone H3 deacetylation
histone H4 deacetylation
positive regulation of cell migration involved in sprouting angiogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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