Invitrogen

HEBP1 Polyclonal Antibody

HEBP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

HEBP1 Antibody (PA5-30609) in ICC/IF

Immunocytochemistry analysis of HEBP1 in methanol-fixed HeLa. Samples were incubated with HEBP1 polyclonal antibody (Product # PA5-30609) using a dilution of 1:400.

HEBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HEBP1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

HEBP1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

HEBP1 Antibody — Figure 8. Neuronal cell death occurs by triggering mitochondrial-dependent apoptotic pathway in Hebp1-expressing neurons. Western blot analyses of (A and B) CytC and Mic60 leakages and (C) caspase 9 activation in Hebp1-deficient, wildtype and control neurons. Wildtype and control neurons exhibited high levels of activated caspase 9 concomitant with mitochondrial release of CytC and Mic60 into the cytosol (S). Hebp1 release was also coupled with leakage of CytC and Mic60 in these cells. In contrast, Hebp1-deficient neurons displayed no apparent activation of caspase 9 despite leakages of CytC and Mic60 from neuronal mitochondria (Mt). (D) Wildtype, control and Hebp1-deficient neurons were treated with 10 µM hemin for 24 hr. Apoptotic cells were visualized by fluorescence staining corresponding to caspase 3/7 activation (see Materials and methods). Hebp1-deficient neurons demonstrated resistance to apoptosis upon heme overload, whereas wildtype and control neurons exhibited high levels of caspase 3/7 activity. (E) Quantification of the data represented by the images shown in (D). All bar charts represent mean ± SEM. Statistical significance in the datasets was assessed by one-way ANOVA followed by Student’s t-test comparison for individual pairs of samples: ****p<0.001. All data shown are representative of results obtained from three independent experiments. {KO}

Product Details

PA5-30609

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human HEBP1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.63 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2548083

Product Specific Information

Recommended positive controls: A549, HeLa, HepG2, HCT116.

Predicted reactivity: Mouse (86%), Pig (84%), Rhesus Monkey (98%), Bovine (84%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The full-length protein encoded by this gene is an intracellular tetrapyrrole-binding protein. This protein includes a natural chemoattractant peptide of 21 amino acids at the N-terminus, which is a natural ligand for formyl peptide receptor-like receptor 2 (FPRL2) and promotes calcium mobilization and chemotaxis in monocytes and dendritic cells.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.

eLife

Yagensky O,Kohansal-Nodehi M,Gunaseelan S,Rabe T,Zafar S,Zerr I,Härtig W,Urlaub H,Chua JJ

Published figure using HEBP1 polyclonal antibody (Product # PA5-30609) in Immunohistochemistry

Tue Aug 27 00:00:00 UTC 2019

Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.

eLife

Yagensky O,Kohansal-Nodehi M,Gunaseelan S,Rabe T,Zafar S,Zerr I,Härtig W,Urlaub H,Chua JJ

Published figure using HEBP1 polyclonal antibody (Product # PA5-30609) in Immunohistochemistry

Tue Aug 27 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: Heme-binding protein 1; p22 HBP; p22HBP

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Gene Aliases: HBP; HEBP; HEBP1

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UniProt ID: (Human) Q9NRV9, (Mouse) Q9R257

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Entrez Gene ID: (Human) 50865, (Mouse) 15199

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Function(s)

heme binding

Process(es)

heme metabolic process circadian rhythm

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HEBP1 Polyclonal Antibody

HEBP1 Antibody (PA5-30609) in ICC/IF

Product Details

PA5-30609

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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