Invitrogen

HEC1 Monoclonal Antibody (9G3.23)

HEC1 Antibody in Immunocytochemistry (ICC/IF)

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HEC1 Antibody (MA1-23308) in ICC/IF

Immunofluorescent analysis of HEC1 (kinetochore, red) in a BHP 2-7 thyroid cancer anaphase cell cultured in complete medium on a glass slide. Cells were fixed with 4% phosphate-buffered paraformaldehyde for 30 min at 37C, permeabilized with 100% ice-cold methanol for 20 min. and blocked with PBS/1% BSA/0.5% Tween 20 at 37C for 30 min. Cells were stained with anti-HEC1, clone 9G3, mouse IgG2a monoclonal antibody (Product # MA1-23308) at a dilution of 1:200 in PBS/1% BSA/0.1% Tween 20 for 60 min at 37C., and then incubated with goat anti-mo... View More

HEC1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

HEC1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

HEC1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with HEC1 siRNA and reduction of signal was observed in Western Blot using MALT1 Monoclonal Antibody (Product # MA1-23308) {KD}

Product Details

MA1-23308

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 6 publications 6 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

1-5 µg/mL

in situ PLA (PLA)

Assay-dependent

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rodent

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

9G3.23

Immunogen

Human HEC1 protein consisting of amino acids 56-642.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2149871

Product Specific Information

Positive control of HELA WCE suggested.

Target Information

Hec1 is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Induction of spontaneous human neocentromere formation and long-term maturation.

The Journal of cell biology

Murillo-Pineda M,Valente LP,Dumont M,Mata JF,Fachinetti D,Jansen LET

MA1-23308 was used in Immunocytochemistry to report a chromosome engineering system for neocentromere formation in human cells and characterize the first experimentally induced human neocentromere at a naive locus.

Mon Mar 01 00:00:00 UTC 2021

Induction of spontaneous human neocentromere formation and long-term maturation.

The Journal of cell biology

Murillo-Pineda M,Valente LP,Dumont M,Mata JF,Fachinetti D,Jansen LET

Mon Mar 01 00:00:00 UTC 2021

CENP-A overexpression promotes aneuploidy with karyotypic heterogeneity.

The Journal of cell biology

Shrestha RL,Rossi A,Wangsa D,Hogan AK,Zaldana KS,Suva E,Chung YJ,Sanders CL,Difilippantonio S,Karpova TS,Karim B,Foltz DR,Fachinetti D,Aplan PD,Ried T,Basrai MA

Published figure using HEC1 monoclonal antibody (Product # MA1-23308) in Immunocytochemistry

Mon Apr 05 00:00:00 UTC 2021

A Biosensor for the Mitotic Kinase MPS1 Reveals Spatiotemporal Activity Dynamics and Regulation.

Current biology : CB

Kuijt TEF,Lambers MLA,Weterings S,Ponsioen B,Bolhaqueiro ACF,Staijen DHM,Kops GJPL

MA1-23308 was used in Immunocytochemistry to develop MPS1sen, a sensitive and specific FRET-based biosensor for MPS1 activity o understand when and where MPS1 activation occurs and how MPS1 signaling is modulated during mitosis.

Mon Oct 05 00:00:00 UTC 2020

Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin.

Nature communications

Mitra S,Bodor DL,David AF,Abdul-Zani I,Mata JF,Neumann B,Reither S,Tischer C,Jansen LET

Published figure using HEC1 monoclonal antibody (Product # MA1-23308) in Immunohistochemistry

Fri Jan 24 00:00:00 UTC 2020

chTOG is a conserved mitotic error correction factor.

eLife

Herman JA,Miller MP,Biggins S

MA1-23308 was used in Western Blot, Immunocytochemistry to demonstrate there is likely a conserved function for the TOG protein family.

Wed Dec 30 00:00:00 UTC 2020

chTOG is a conserved mitotic error correction factor.

eLife

Herman JA,Miller MP,Biggins S

MA1-23308 was used in Western Blot, Immunocytochemistry to demonstrate there is likely a conserved function for the TOG protein family.

Wed Dec 30 00:00:00 UTC 2020

chTOG is a conserved mitotic error correction factor.

eLife

Herman JA,Miller MP,Biggins S

MA1-23308 was used in Western Blot, Immunocytochemistry to demonstrate there is likely a conserved function for the TOG protein family.

Wed Dec 30 00:00:00 UTC 2020

chTOG is a conserved mitotic error correction factor.

eLife

Herman JA,Miller MP,Biggins S

MA1-23308 was used in Western Blot, Immunocytochemistry to demonstrate there is likely a conserved function for the TOG protein family.

Wed Dec 30 00:00:00 UTC 2020

Reprogramming of human cells to pluripotency induces CENP-A chromatin depletion.

Open biology

Milagre I,Pereira C,Oliveira RA,Jansen LET

MA1-23308 was used in Immunocytochemistry, Western Blot to explore the composition of the centromere and kinetochore in human embryonic and induced PSCs.

Thu Oct 01 00:00:00 UTC 2020

Reprogramming of human cells to pluripotency induces CENP-A chromatin depletion.

Open biology

Milagre I,Pereira C,Oliveira RA,Jansen LET

MA1-23308 was used in Immunocytochemistry, Western Blot to explore the composition of the centromere and kinetochore in human embryonic and induced PSCs.

Thu Oct 01 00:00:00 UTC 2020

Reprogramming of human cells to pluripotency induces CENP-A chromatin depletion.

Open biology

Milagre I,Pereira C,Oliveira RA,Jansen LET

MA1-23308 was used in Immunocytochemistry, Western Blot to explore the composition of the centromere and kinetochore in human embryonic and induced PSCs.

Thu Oct 01 00:00:00 UTC 2020

The quantitative architecture of centromeric chromatin.

eLife

Bodor DL,Mata JF,Sergeev M,David AF,Salimian KJ,Panchenko T,Cleveland DW,Black BE,Shah JV,Jansen LE

MA1-23308 was used in immunocytochemistry to study the localization of CENP-A in the cell and at centromeres.

Tue Jul 15 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: HEC-1; HEC2; HEC4; Highly expressed in cancer protein; highly expressed in cancer, rich in leucine heptad repeats; HsHec1; kinetochore associated 2; Kinetochore protein Hec1; Kinetochore protein NDC80 homolog; Kinetochore-associated protein 2; NDC80 homolog, kinetochore complex component; NDC80 kinetochore complex component; NDC80 kinetochore complex component homolog; putative kinetochore protein NDC80 like protein; Retinoblastoma-associated protein HEC

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Gene Aliases: 2610020P18Rik; AV139730; HEC; HEC1; HsHec1; hsNDC80; KNTC2; NDC80; TID3

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UniProt ID: (Human) O14777, (Mouse) Q9D0F1

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Entrez Gene ID: (Human) 10403, (Chinese hamster) 100751668, (Mouse) 67052

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Function(s)

structural constituent of cytoskeleton protein binding identical protein binding

Process(es)

mitotic sister chromatid segregation establishment of mitotic spindle orientation mitotic spindle organization chromosome segregation sister chromatid cohesion mitotic nuclear division attachment of spindle microtubules to kinetochore cell division attachment of mitotic spindle microtubules to kinetochore cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HEC1 Monoclonal Antibody (9G3.23)

HEC1 Antibody (MA1-23308) in ICC/IF

Product Details

MA1-23308

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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