Invitrogen

HELIOS Monoclonal Antibody (22F6), PE, eBioscience™

11 Published Figures

19 References

View all (22) HELIOS antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (11)

HELIOS Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 12

HELIOS Antibody (12-9883-42) in Flow

Intracellular staining of C57Bl/6 mouse lymph node cells with Anti-Mouse/Rat Foxp3 eFluor® 450 (Product # 48-5773-82) and Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (left) or Anti-Helios PE (right) using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. CD4+ (Product # 17-0041-82) cells in the lymphocyte gate were used for analysis.

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Product Details

12-9883-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.03 µg)/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

22F6

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2572758

Product Specific Information

Description: This 22F6 monoclonal antibody reacts with human, mouse and canine Helios. Helios is a zinc-finger transcription factor first identified for its similarity to Ikaros. It is expressed in hematopoietic stem cells in both the embryo and adult mice. While the highest expression is found in regulatory T cells, Helios is not expressed in mature B cells, dendritic cells or myeloid cells. Helios can form a homodimer as well as heterodimerize with other Ikaros family members to regulate transcription. Eight isoforms of Helios exist, some of which possess a dominant-negative function that has also been found to be upregulated in lymphoid malignancies, including T-acute lymphoblastic leukemia.

22F6 has been reported to cross react with rat Helios.

Applications Reported: This 22F6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 22F6 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse lymph node cell using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Dexmedetomidine alleviates acute lung injury by promoting Tregs differentiation via activation of AMPK/SIRT1 pathway.

Inflammopharmacology

Zhang ZT,Xie K,Luo RJ,Zhang DY,He ZW,Li KF,Lin SH,Xu F

12-9883-42 was used in Flow cytometry/Cell sorting to explore the anti-inflammatory effect and the potential mechanism of dexmedetomidine in ARDS/ALI.

Wed Feb 01 00:00:00 UTC 2023

Dexmedetomidine alleviates acute lung injury by promoting Tregs differentiation via activation of AMPK/SIRT1 pathway.

Inflammopharmacology

Zhang ZT,Xie K,Luo RJ,Zhang DY,He ZW,Li KF,Lin SH,Xu F

12-9883-42 was used in Flow cytometry/Cell sorting to explore the anti-inflammatory effect and the potential mechanism of dexmedetomidine in ARDS/ALI.

Wed Feb 01 00:00:00 UTC 2023

Interaction between tissue-dwelling helminth and the gut microbiota drives mucosal immunoregulation.

NPJ biofilms and microbiomes

Wang Y,Guo A,Zou Y,Mu W,Zhang S,Shi Z,Liu Z,Cai X,Zhu XQ,Wang S

Published figure using HELIOS monoclonal antibody (Product # 12-9883-42) in Flow Cytometry

Sat Jun 24 00:00:00 UTC 2023

Activation-induced cytidine deaminase expression by thymic B cells promotes T-cell tolerance and limits autoimmunity.

iScience

Lombard-Vadnais F,Chabot-Roy G,Zahn A,Rodriguez Torres S,Di Noia JM,Melichar HJ,Lesage S

Published figure using HELIOS monoclonal antibody (Product # 12-9883-42) in Flow Cytometry

Fri Jan 20 00:00:00 UTC 2023

Mesenchymal stem cells transfer mitochondria to allogeneic Tregs in an HLA-dependent manner improving their immunosuppressive activity.

Nature communications

Piekarska K,Urban-Wójciuk Z,Kurkowiak M,Pelikant-Małecka I,Schumacher A,Sakowska J,Spodnik JH,Arcimowicz Ł,Zielińska H,Tymoniuk B,Renkielska A,Siebert J,Słomińska E,Trzonkowski P,Hupp T,Marek-Trzonkowska NM

Published figure using HELIOS monoclonal antibody (Product # 12-9883-42) in Flow Cytometry

Mon Feb 14 00:00:00 UTC 2022

Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections.

Immunity

Harb H,Benamar M,Lai PS,Contini P,Griffith JW,Crestani E,Schmitz-Abe K,Chen Q,Fong J,Marri L,Filaci G,Del Zotto G,Pishesha N,Kolifrath S,Broggi A,Ghosh S,Gelmez MY,Oktelik FB,Cetin EA,Kiykim A,Kose M,Wang Z,Cui Y,Yu XG,Li JZ,Berra L,Stephen-Victor E,Charbonnier LM,Zanoni I,Ploegh H,Deniz G,De Palma R,Chatila TA

Published figure using HELIOS monoclonal antibody (Product # 12-9883-42) in Flow Cytometry

Tue Jun 08 00:00:00 UTC 2021

Potential therapeutic effects of interleukin-35 on the differentiation of naïve T cells into Helios⁺Foxp3⁺ Tregs in clinical and experimental acute respiratory distress syndrome.

Molecular immunology

Wang C,Xie K,Li K,Lin S,Xu F

Published figure using HELIOS monoclonal antibody (Product # 12-9883-42) in Flow Cytometry

Thu Apr 01 00:00:00 UTC 2021

Adiponectin/AdipoR1 Axis Promotes IL-10 Release by Human Regulatory T Cells.

Frontiers in immunology

Ramos-Ramírez P,Malmhäll C,Tliba O,Rådinger M,Bossios A

12-9883-42 was used in Flow Cytometry to suggest that adiponectin/AdipoR1 axis promotes IL-10 release by Tregs, mainly in Helios- Tregs, and the effect was amplified by T2 inflammation in Helios+ Tregs.

Tue Oct 26 00:00:00 UTC 2021

Adiponectin/AdipoR1 Axis Promotes IL-10 Release by Human Regulatory T Cells.

Frontiers in immunology

Ramos-Ramírez P,Malmhäll C,Tliba O,Rådinger M,Bossios A

Tue Oct 26 00:00:00 UTC 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

12-9883-42 was used in Flow Cytometry to explore the immunological changes in circulating Helios+ and Helios- regulator T-cells in type 1 diabetes.

Wed Sep 15 00:00:00 UTC 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

12-9883-42 was used in Flow Cytometry to explore the immunological changes in circulating Helios+ and Helios- regulator T-cells in type 1 diabetes.

Wed Sep 15 00:00:00 UTC 2021

A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma.

Nature immunology

Harb H,Stephen-Victor E,Crestani E,Benamar M,Massoud A,Cui Y,Charbonnier LM,Arbag S,Baris S,Cunnigham A,Leyva-Castillo JM,Geha RS,Mousavi AJ,Guennewig B,Schmitz-Abe K,Sioutas C,Phipatanakul W,Chatila TA

Published figure using HELIOS monoclonal antibody (Product # 12-9883-42) in Flow Cytometry

Sun Nov 01 00:00:00 UTC 2020

Distinct actions of the fermented beverage kefir on host behaviour, immunity and microbiome gut-brain modules in the mouse.

Microbiome

van de Wouw M,Walsh AM,Crispie F,van Leuven L,Lyte JM,Boehme M,Clarke G,Dinan TG,Cotter PD,Cryan JF

12-9883 was used in Flow cytometry/Cell sorting to indicate that kefir can positively modulate specific aspects of the microbiota-gut-brain axis and support the broadening of the definition of psychobiotic to include kefir fermented foods.

Mon May 18 00:00:00 UTC 2020

Distinct actions of the fermented beverage kefir on host behaviour, immunity and microbiome gut-brain modules in the mouse.

Microbiome

van de Wouw M,Walsh AM,Crispie F,van Leuven L,Lyte JM,Boehme M,Clarke G,Dinan TG,Cotter PD,Cryan JF

Mon May 18 00:00:00 UTC 2020

B7-CD28 co-stimulation modulates central tolerance via thymic clonal deletion and Treg generation through distinct mechanisms.

Nature communications

Watanabe M,Lu Y,Breen M,Hodes RJ

Published figure using HELIOS monoclonal antibody (Product # 12-9883-42) in Flow Cytometry

Tue Dec 08 00:00:00 UTC 2020

CD19-targeted CAR regulatory T cells suppress B cell pathology without GvHD.

JCI insight

Imura Y,Ando M,Kondo T,Ito M,Yoshimura A

12-9883 was used in Flow cytometry/Cell sorting to suggest that CD19-CAR regulatory T cells can treat autoantibody-mediated autoimmune diseases by suppressing B cell pathology.

Thu Jul 23 00:00:00 UTC 2020

Umbilical cord blood‑derived Helios‑positive regulatory T cells promote angiogenesis in acute lymphoblastic leukemia in mice via CCL22 and the VEGFA‑VEGFR2 pathway.

Molecular medicine reports

Li X,Li D,Shi Q,Huang X,Ju X

12-9883 was used in Flow cytometry/Cell sorting to elucidate the mechanisms underlying the role of regulatory T cells in acute lymphoblastic leukaemia.

Wed May 01 00:00:00 UTC 2019

CD150^high Bone Marrow Tregs Maintain Hematopoietic Stem Cell Quiescence and Immune Privilege via Adenosine.

Cell stem cell

Hirata Y,Furuhashi K,Ishii H,Li HW,Pinho S,Ding L,Robson SC,Frenette PS,Fujisaki J

12-9883-42 was used in Flow Cytometry to describe a Treg subpopulation that controls hematopoietic stem cell (HSC) quiescence and engraftment.

Thu Mar 01 00:00:00 UTC 2018

Ablation of Transcription Factor IRF4 Promotes Transplant Acceptance by Driving Allogenic CD4⁺ T Cell Dysfunction.

Immunity

Wu J,Zhang H,Shi X,Xiao X,Fan Y,Minze LJ,Wang J,Ghobrial RM,Xia J,Sciammas R,Li XC,Chen W

12-9883-42 was used in Flow Cytometry to demonstrate that interferon regulatory factor 4 was a key transcriptional determinant controlling T cell responses during transplantation.

Tue Dec 19 00:00:00 UTC 2017

Decreased Helios Expression in Regulatory T Cells in Acute Coronary Syndrome.

Disease markers

Jiang L,Chen F,Hu X,Hu Y,Wang Y,Zhang W,Peng Y,Cheng L

12-9883 was used in Flow cytometry/Cell sorting to study the role of Helios in acute coronary syndrome.

Fri Aug 03 00:00:00 UTC 2018

Foxp3 and Toll-like receptor signaling balance T_reg cell anabolic metabolism for suppression.

Nature immunology

Gerriets VA,Kishton RJ,Johnson MO,Cohen S,Siska PJ,Nichols AG,Warmoes MO,de Cubas AA,MacIver NJ,Locasale JW,Turka LA,Wells AD,Rathmell JC

12-9883 was used in Flow cytometry/Cell sorting to demonstrate that inflammatory signals and Foxp3 balance mTORC1 signaling and glucose metabolism to control the proliferation and suppressive function of Treg cells.

Thu Dec 01 00:00:00 UTC 2016

Human adipose-tissue derived mesenchymal stem cells induce functional de-novo regulatory T cells with methylated FOXP3 gene DNA.

Clinical and experimental immunology

Engela AU,Hoogduijn MJ,Boer K,Litjens NH,Betjes MG,Weimar W,Baan CC

12-9883 was used in Flow cytometry/Cell sorting to study whether human mesenchymal stem cell-induced CD4(+) CD25(+) CD127(-) forkhead box P3(+) T cells are functional and originate from effector T cells or represent expanded natural regulatory T cells.

Thu Aug 01 00:00:00 UTC 2013

Helios is associated with CD4 T cells differentiating to T helper 2 and follicular helper T cells in vivo independently of Foxp3 expression.

PloS one

Serre K,Bénézech C,Desanti G,Bobat S,Toellner KM,Bird R,Chan S,Kastner P,Cunningham AF,Maclennan IC,Mohr E

12-9883 was used in Flow cytometry/Cell sorting to reveal the selective upregulation of Helios during Th2 and TFh responses to alum-protein vaccines.

Mon Oct 17 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: IKAROS family zinc finger 2 (Helios); Ikaros family zinc finger protein 2; zinc finger DNA binding protein Helios; Zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios)

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Gene Aliases: A730095J18Rik; ANF1A2; HELIOS; IKZF2; Zfpn1a2; ZNF1A2; ZNFN1A2

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UniProt ID: (Human) Q9UKS7, (Mouse) P81183

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Entrez Gene ID: (Dog) 488510, (Human) 22807, (Mouse) 22779

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Function(s)

molecular_function transcription factor activity, sequence-specific DNA binding protein binding protein homodimerization activity transcription regulatory region DNA binding metal ion binding protein heterodimerization activity nucleic acid binding DNA binding

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HELIOS Monoclonal Antibody (22F6), PE, eBioscience™

HELIOS Antibody (12-9883-42) in Flow

Product Details

12-9883-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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