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Invitrogen
HENMT1 Polyclonal Antibody
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FIGURE: 1 / 3
HENMT1 Antibody (PA5-28543) in ICC/IF
Immunocytochemistry (Confocal immunofluorescence) of HENMT1 in methanol-fixed HeLa. Samples were incubated with HENMT1 polyclonal antibody (Product # PA5-28543) using a dilution of 1:500 (green). Alpha-tubulin filaments were labeled with alpha Tubulin monoclonal antibody (Product # MA1-25054) (Red) at 1:2,000.
Product Details
PA5-28543
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human HENMT1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2546019
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.
Predicted reactivity: Rhesus Monkey (99%), Chimpanzee (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HENMT1 (HEN Methyltransferase 1) is a small RNA methyltransferase gene crucial for the stability and functionality of PIWI-interacting RNAs (piRNAs). It is located in human chromosome 1 at the p36.31 locus. The HENMT1 protein catalyzes the 2'-O-methylation at the 3' end of piRNAs in a Mg2+ dependent manner. This modification is essential for piRNA maturation and stability, ensuring the repression of transposable elements (TEs) during spermatogenesis and maintaining genomic integrity in male germ cells. Studies have highlighted the impact of HENMT1 mutations causing male infertility due to disrupted piRNA pathway and improper germ cell gene regulation. The enzymatic activity of HENMT1 is not only significant for male fertility but is also involved in maintaining piRNA stability in female germ cells, although females with HENMT1 mutations show subfertility.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HEN1 methyltransferase homolog 1; hua enhancer 1 homolog 1; Small RNA 2'-O-methyltransferase
Gene Aliases: C1orf59; HEN1; HENMT1
UniProt ID: (Human) Q5T8I9
Entrez Gene ID: (Human) 113802
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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