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HEPACAM Antibody (PA5-88157) in WB
Western blot analysis of HEPACAM in extracts of various cell lines. Samples were incubated with HEPACAM Polyclonal antibody (Product # PA5-88157) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-88157
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 35-245 of human HEPACAM (NP_6899352)
Conjugate
Form
Liquid
Concentration
2.16 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804699
Product Specific Information
Immunogen sequence: NITSPVRLIH GTVGKSALLS VQYSSTSSDR PVVKWQLKRD KPVTVVQSIG TEVIGTLRPD YRDRIRLFEN GSLLLSDLQL ADEGTYEVEI SITDDTFTGE KTINLTVDVP ISRPQVLVAS TTVLELSEAF TLNCSHENGT KPSYTWLKDG KPLLNDSRML LSPDQKVLTI TRVLMEDDDL YSCMVENPIS QGRSLPVKIT VYRRSSLYII L; Positive Samples: 293T, HepG2, MCF-7, SH-SY5Y, SGC-7901, Mouse brain, Mouse liver, Rat brain, Rat liver; Cellular Location: Cytoplasm, Cytoplasmic side, Membrane, Single-pass type I membrane protein
Target Information
HEPACAM is involved in regulating cell motility and cell-matrix interactions. HEPACAM may inhibit cell growth through suppression of cell proliferation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glialCAM; HEPACAM; Hepatic and glial cell adhesion molecule; Hepatocyte cell adhesion molecule; Protein hepaCAM
Gene Aliases: 2900042E01Rik; GlialCAM; HEPACAM; Hepn1; MLC2A; MLC2B; RGD1306811
UniProt ID: (Human) Q14CZ8, (Mouse) Q640R3
Entrez Gene ID: (Human) 220296, (Mouse) 72927, (Rat) 300517
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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