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HERC2 Antibody (A301-905A) in WB
Detection of human HERC2 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, MCF-7, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC2 antibody (Product # A301-905A lot 3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A301-905A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 4784 and 4834 of human hect domain and RLD 2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority if the nuclear volume. Required for maintenance and establishment of the mitotic spindle poles, functionning as a tether linking bulk microtubules of the spindle to centrosomes. May be involved in coordination of the alignment of the mitotic spindle to the cellular polarity axis, which is a prerequisite for asymmetric cell divisions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase HERC2; HECT domain and RCC1-like domain-containing protein 2; hect domain and RLD 2; HECT-type E3 ubiquitin transferase HERC2; HERC2; probable E3 ubiquitin-protein ligase HERC2
Gene Aliases: D15F32S1h; D15F37S1; D7H15F32S1; D7H15F37S1; HERC2; Jdf2; Kiaa0393; mKIAA0393; MRT38; p528; Rjs; SHEP1
UniProt ID: (Human) O95714, (Mouse) Q4U2R1
Entrez Gene ID: (Human) 8924, (Mouse) 15204
ubiquitin-protein transferase activity
guanyl-nucleotide exchange factor activity
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase binding
SUMO binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
DNA repair
cellular response to DNA damage stimulus
spermatogenesis
protein ubiquitination
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
double-strand break repair via nonhomologous end joining
intracellular protein transport
protein sumoylation
positive regulation of GTPase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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