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Invitrogen
HERC5 Monoclonal Antibody (K29053_7B12)
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HERC5 Antibody (MA5-55796) in WB
Western blot analysis of HERC5 in 15 µg of HEK-293 lysate. Sample was run on 6-18% SDS-PAGE under reducing conditions, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. HERC5 band was visualized using ECL Western Blotting Substrate. Incubation with primary HERC5 monoclonal antibody (Product # MA5-55796) at a dilution of 1 µg/mL was used.
Product Details
MA5-55796
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
K29053_7B12
Immunogen
Recombinant human HERC5 (immunogen range is 671-1020).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_3668028
Product Specific Information
Sequence of this protein is as follows: RESEFALRPT FDLTVRRNHL IEDVLNQLSQ FENEDLRKEL WVSFSGEIGY DLGGVKKEFF YCLFAEMIQP EYGMFMYPEG ASCMWFPVKP KFEKKRYFFF GVLCGLSLFN CNVANLPFPL ALFKKLLDQM PSLEDLKELS PDLGKNLQTL LDDEGDNFEE VFYIHFNVHW DRNDTNLIPN GSSITVNQTN KRDYVSKYIN YIFNDSVKAV YEEFRRGFYK MCDEDIIKLF HPEELKDVIV GNTDYDWKTF EKNARYEPGY NSSHPTIVMF WKAFHKLTLE EKKKFLVFLT GTDRLQMKDL NNMKITFCCP ESWNERDPIR ALTCFSVLFL PKYSTMETVE EALQEAINNN
Target Information
HERC5 (E3 ISG15 - protein ligase HERC5) is a major E3 ligase for ISG15 conjugation. It acts a positive regulator of innate antiviral response in cells induced by interferon. HERC5 functions as a part of the ISGylation machinery that recognizes target protein in a broad and relatively non-specific manner. It catalyzes ISGylation of IRF3 - which results in sustained activation, it attenuates IRF3-PIN1 interaction - which antagonizes IFR3 ubiquitination and degradation, and boosts the antiviral repsonse. HERC5 is physically assocated with polyribosomes and broadly modifies newly synthesized proteins in a cotranslational manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cyclin-E-binding protein 1; E3 ISG15--protein ligase HERC5; HECT domain and RCC1-like domain-containing protein 5; hect domain and RLD 5; probable E3 ubiquitin-protein ligase HERC5
Gene Aliases: CEB1; CEBP1; HERC5
UniProt ID: (Human) Q9UII4
Entrez Gene ID: (Human) 51191
regulation of cyclin-dependent protein serine/threonine kinase activity
protein polyubiquitination
ISG15-protein conjugation
negative regulation of type I interferon production
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
innate immune response
regulation of defense response to virus
defense response to virus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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