Invitrogen

HEY1 Polyclonal Antibody

5 Published Figures

7 References

View all (23) HEY1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (5)

HEY1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

HEY1 Antibody (PA5-40553) in IHC (P)

Immunohistochemistry (paraffin-embedded) analysis of human kidney tissue using an anti-HEY1 polyclonal antibody (Product # PA5-40553).

HEY1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

HEY1 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

PA5-40553

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

4-8 µg/mL

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the C-terminal of human HEY1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2576326

Product Specific Information

Peptide sequence: PQNGHGNAGT TASPTEPHHQ GRLGSAHPEA PALRAPPSGS FGPVLPVVTS

Sequence homology: Cow: 79%; Dog: 86%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 79%; Rat: 93%

Target Information

Hairy/enhancer-of-split related with YRPW motif protein 1 belongs to the HEY family and is a Notch Signaling Pathway Protein. It contains one basic helix-loop-helix (bHLH) domain and one Orange domain. It is a downstream effector of Notch signaling which may be required for cardiovascular development. It acts as transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. It represses transcription by the cardiac transcriptional activators GATA4 and GATA6. Hey1 is also reported to be expressed during development of the nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Crosstalk between Androgen-ZIP9 Signaling and Notch Pathway in Rodent Sertoli Cells.

International journal of molecular sciences

Kamińska A,Marek S,Pardyak L,Brzoskwinia M,Bilinska B,Hejmej A

Published figure using HEY1 polyclonal antibody (Product # PA5-40553) in Immunocytochemistry

Thu Nov 05 00:00:00 UTC 2020

Crosstalk between Androgen-ZIP9 Signaling and Notch Pathway in Rodent Sertoli Cells.

International journal of molecular sciences

Kamińska A,Marek S,Pardyak L,Brzoskwinia M,Bilinska B,Hejmej A

Published figure using HEY1 polyclonal antibody (Product # PA5-40553) in Immunocytochemistry

Thu Nov 05 00:00:00 UTC 2020

Disruption of androgen signaling during puberty affects Notch pathway in rat seminiferous epithelium.

Reproductive biology and endocrinology : RB&E

Kamińska A,Marek S,Pardyak L,Brzoskwinia M,Pawlicki P,Bilińska B,Hejmej A

PA5-40553 was used in Immunohistochemistry to examine whether activation of Notch receptors and expression of Notch ligands and effector genes in rat seminiferous epithelium are controlled by androgen signaling during puberty.

Thu Apr 16 00:00:00 UTC 2020

Disruption of androgen signaling during puberty affects Notch pathway in rat seminiferous epithelium.

Reproductive biology and endocrinology : RB&E

Kamińska A,Marek S,Pardyak L,Brzoskwinia M,Pawlicki P,Bilińska B,Hejmej A

Thu Apr 16 00:00:00 UTC 2020

Disruption of androgen signaling during puberty affects Notch pathway in rat seminiferous epithelium.

Reproductive biology and endocrinology : RB&E

Kamińska A,Marek S,Pardyak L,Brzoskwinia M,Pawlicki P,Bilińska B,Hejmej A

Thu Apr 16 00:00:00 UTC 2020

Notch signaling regulates nuclear androgen receptor AR and membrane androgen receptor ZIP9 in mouse Sertoli cells.

Andrology

Kamińska A,Pardyak L,Marek S,Wróbel K,Kotula-Balak M,Bilińska B,Hejmej A

PA5-40553 was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate that AR expression is regulated by DLL1-mediated Notch signaling, whereas JAG1 is involved in the regulation of ZIP9.

Sun Mar 01 00:00:00 UTC 2020

Notch signaling regulates nuclear androgen receptor AR and membrane androgen receptor ZIP9 in mouse Sertoli cells.

Andrology

Kamińska A,Pardyak L,Marek S,Wróbel K,Kotula-Balak M,Bilińska B,Hejmej A

Sun Mar 01 00:00:00 UTC 2020

Human blood vessel organoids as a model of diabetic vasculopathy.

Nature

Wimmer RA,Leopoldi A,Aichinger M,Wick N,Hantusch B,Novatchkova M,Taubenschmid J,Hämmerle M,Esk C,Bagley JA,Lindenhofer D,Chen G,Boehm M,Agu CA,Yang F,Fu B,Zuber J,Knoblich JA,Kerjaschki D,Penninger JM

PA5-40553 was used in Immunocytochemistry-immunoflourescence to suggest that blood vessel organoids derived from human stem cells faithfully recapitulate the structure and function of human blood vessels and are a suitable model for diabetic vasculopathy.

Tue Jan 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: basic helix-loop-helix protein OAF1; bHLHb31; Cardiovascular helix-loop-helix factor 2; CHF-2; Class B basic helix-loop-helix protein 31; Hairy and enhancer of split-related protein 1; Hairy-related transcription factor 1; hairy/enhancer-of-split related with YRPW motif 1; Hairy/enhancer-of-split related with YRPW motif protein 1; hes-related family bHLH transcription factor with YRPW motif 1; HES-related repressor protein 1; HES-related repressor protein 2; HESR-1; HRT-1; MGC1274

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Gene Aliases: BHLHB31; CHF2; HERP2; HESR1; HEY1; hHRT1; HRT-1; HRT1; OAF1

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UniProt ID: (Human) Q9Y5J3

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Entrez Gene ID: (Human) 23462

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Function(s)

transcription factor activity, RNA polymerase II core promoter sequence-specific transcription factor activity, protein binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding microsatellite binding protein dimerization activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter angiogenesis pulmonary valve morphogenesis atrioventricular valve formation epithelial to mesenchymal transition involved in endocardial cushion formation endocardial cushion morphogenesis cardiac ventricle morphogenesis transcription, DNA-templated Notch signaling pathway dorsal aorta morphogenesis umbilical cord morphogenesis negative regulation of Notch signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cardiac epithelial to mesenchymal transition heart trabecula formation cardiac septum morphogenesis ventricular septum morphogenesis labyrinthine layer blood vessel development arterial endothelial cell differentiation Notch signaling involved in heart development cellular response to glucocorticoid stimulus negative regulation of transcription regulatory region DNA binding negative regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation regulation of vasculogenesis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HEY1 Polyclonal Antibody

HEY1 Antibody (PA5-40553) in IHC (P)

Product Details

PA5-40553

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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