Invitrogen

HGF Recombinant Rabbit Monoclonal Antibody (7H 6L1)

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2 References
View all (38) HGF antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

701283

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-1 µg/mL
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL
-

ELISA (ELISA)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

7H 6L1

Immunogen

Recombinant protein corresponding to amino acids 27-169 of human HGF
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532457

Product Specific Information

This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Fibroblast derived tumor cytotoxic factor; fibroblast-derived tumor cytotoxic factor; Hepatocyte growth factor; Hepatocyte growth factor (hepapoietin A; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin A; Hepatopoietin-A; HepatopoietinA; Lung fibroblast derived mitogen; lung fibroblast-derived mitogen; Scatter factor; scatter factor); SF

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Gene Aliases: DFNB39; F-TCF; HGF; HGFB; HPTA; SF

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UniProt ID: (Human) P14210

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Entrez Gene ID: (Human) 3082

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Function(s)
serine-type endopeptidase activity protein binding growth factor activity chemoattractant activity protein heterodimerization activity
Process(es)
activation of MAPK activity epithelial to mesenchymal transition liver development platelet degranulation proteolysis mitotic nuclear division positive regulation of cell proliferation negative regulation of autophagy positive regulation of phosphatidylinositol 3-kinase signaling hyaluronan metabolic process positive regulation of cell migration organ regeneration positive regulation of myelination negative regulation of interleukin-6 production positive regulation of interleukin-10 production negative regulation of peptidyl-serine phosphorylation cellular response to hepatocyte growth factor stimulus negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of osteoblast differentiation positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter hepatocyte growth factor receptor signaling pathway negative regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis myoblast proliferation cell chemotaxis regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling positive regulation of neuron projection regeneration negative regulation of release of cytochrome c from mitochondria regulation of p38MAPK cascade negative regulation of hydrogen peroxide-mediated programmed cell death negative regulation of extrinsic apoptotic signaling pathway via death domain receptors regulation of tau-protein kinase activity positive regulation of DNA biosynthetic process
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