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HHIP Antibody (PA5-29529) in WB
Western Blot using HHIP Polyclonal Antibody (Product # PA5-29529). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP Polyclonal Antibody (Product # PA5-29529) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-29529
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human HHIP. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.89 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547005
Product Specific Information
Recommended positive controls: NT2D1.
Predicted reactivity: Mouse (97%), Rat (96%), Rhesus Monkey (99%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a protein similar to the mouse hedgehog-interacting protein, a regulatory component of the hedgehog signaling pathway. Members of the hedgehog family are evolutionarily conserved proteins which are involved in many fundamental processes in embryonic development, including anteroposterior patterns of limbs and regulation of left-right asymmetry.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hedgehog-interacting protein; HHIP
Gene Aliases: HHIP; HIP; UNQ5825/PRO19644
UniProt ID: (Human) Q96QV1
Entrez Gene ID: (Human) 64399
carbohydrate metabolic process
smoothened signaling pathway
neuroblast proliferation
dorsal/ventral pattern formation
negative regulation of signal transduction
regulation of fibroblast growth factor receptor signaling pathway
negative regulation of smoothened signaling pathway
skeletal system morphogenesis
oxidation-reduction process
epithelial tube branching involved in lung morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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