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HIC5 Polyclonal Antibody

View all (13) HIC5 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (6)

HIC5 Antibody in Immunocytochemistry (ICC/IF)

HIC5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

HIC5 Antibody (10565-1-AP) in ICC/IF

Immunofluorescent analysis of A431 cells using 10565-1-AP (HIC5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

HIC5 Antibody in Immunocytochemistry (ICC/IF)

HIC5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HIC5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HIC5 Antibody in Western Blot (WB)

HIC5 Antibody in Western Blot (WB)

HIC5 Antibody in Immunoprecipitation (IP)

Product Image Gallery

HIC5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of A431 cells using 10565-1-AP (HIC5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

HIC5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human spleen tissue slide using 10565-1-AP ( HIC5 antibody at dilution of 1:50 (under 40x lens).

HIC5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human spleen tissue slide using 10565-1-AP ( HIC5 antibody at dilution of 1:50 (under 10x lens).

HIC5 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 10565-1-AP) HIC5 antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

HIC5 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10565-1-AP (HIC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HIC5 Antibody in Immunoprecipitation (IP) — IP result of anti-HIC5 (IP:10565-1-AP, 4ug; Detection:10565-1-AP 1:500) with A431 cells lysate 1520ug.

Product Details

10565-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HIC5 Fusion Protein Ag0868 (1-230 aa encoded by BC001830)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.44 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MPRSGAPKE RPAEPLTPPP SYGHQPQTGS GESSGASGDK DHLYSTVCKP RSPKPAAPAA PPFSSSSGVL GTGLCELDRL LQELNATQFN ITDEIMSQFP SSKVASGEQK EDQSEDKKRP SLPSSPSPGL PKASATSATL ELDRLMASLS DFRVQNHLPA SGPTQPPVVS STNEGSPSPP EPTGKGSLDT MLGLLQSDLS RRGVPTQAKG LCGSCNKPIA GQVVTALGRA W (1-230 aa encoded by BC001830)

Target Information

This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy.

Acta pharmacologica Sinica

Wu JC,Wang XJ,Zhu JH,Huang XY,Liu M,Qiao Z,Zhang Y,Sun Y,Wang ZY,Zhan P,Zhang T,Hu HL,Liu H,Tang W,Yi F

Thu Jun 01 00:00:00 UTC 2023

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy.

Acta pharmacologica Sinica

Wu JC,Wang XJ,Zhu JH,Huang XY,Liu M,Qiao Z,Zhang Y,Sun Y,Wang ZY,Zhan P,Zhang T,Hu HL,Liu H,Tang W,Yi F

Thu Jun 01 00:00:00 UTC 2023

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy.

Acta pharmacologica Sinica

Wu JC,Wang XJ,Zhu JH,Huang XY,Liu M,Qiao Z,Zhang Y,Sun Y,Wang ZY,Zhan P,Zhang T,Hu HL,Liu H,Tang W,Yi F

Thu Jun 01 00:00:00 UTC 2023

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy.

Acta pharmacologica Sinica

Wu JC,Wang XJ,Zhu JH,Huang XY,Liu M,Qiao Z,Zhang Y,Sun Y,Wang ZY,Zhan P,Zhang T,Hu HL,Liu H,Tang W,Yi F

Thu Jun 01 00:00:00 UTC 2023

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy.

Acta pharmacologica Sinica

Wu JC,Wang XJ,Zhu JH,Huang XY,Liu M,Qiao Z,Zhang Y,Sun Y,Wang ZY,Zhan P,Zhang T,Hu HL,Liu H,Tang W,Yi F

Thu Jun 01 00:00:00 UTC 2023

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy.

Acta pharmacologica Sinica

Wu JC,Wang XJ,Zhu JH,Huang XY,Liu M,Qiao Z,Zhang Y,Sun Y,Wang ZY,Zhan P,Zhang T,Hu HL,Liu H,Tang W,Yi F

Thu Jun 01 00:00:00 UTC 2023

Bioinformatics Profiling and Experimental Validation of 4 Differentially-Expressed LIM Genes in the Course of Colorectal-Adenoma-Carcinoma.

Medical science monitor : international medical journal of experimental and clinical research

He Y,Pan Z,Shi Q,Zhang X,Shen W,Huo L,Guo H,Tang C,Ling Y

Wed Jul 20 00:00:00 UTC 2022

Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.

Aging

Sha L,Ma D,Chen C

Sun Dec 13 00:00:00 UTC 2020

Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.

Aging

Sha L,Ma D,Chen C

Sun Dec 13 00:00:00 UTC 2020

Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.

Aging

Sha L,Ma D,Chen C

Sun Dec 13 00:00:00 UTC 2020

HIC-5 in cancer-associated fibroblasts contributes to esophageal squamous cell carcinoma progression.

Cell death & disease

Du X,Xu Q,Pan D,Xu D,Niu B,Hong W,Zhang R,Li X,Chen S

Mon Nov 18 00:00:00 UTC 2019

HIC-5 in cancer-associated fibroblasts contributes to esophageal squamous cell carcinoma progression.

Cell death & disease

Du X,Xu Q,Pan D,Xu D,Niu B,Hong W,Zhang R,Li X,Chen S

Mon Nov 18 00:00:00 UTC 2019

Quantitative Proteomics Implicates Rictor/mTORC2 in Cell Adhesion.

Journal of proteome research

Wang H,Shao X,He Q,Wang C,Xia L,Yue D,Qin G,Jia C,Chen R

Fri Oct 05 00:00:00 UTC 2018

Quantitative Proteomics Implicates Rictor/mTORC2 in Cell Adhesion.

Journal of proteome research

Wang H,Shao X,He Q,Wang C,Xia L,Yue D,Qin G,Jia C,Chen R

Fri Oct 05 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: Androgen receptor coactivator 55 kDa protein; androgen receptor coactivator ARA55; Androgen receptor-associated protein of 55 kDa; Hic-5; Hydrogen peroxide-inducible clone 5 protein; hydrogen peroxide-inducible clone-5; TGF beta-stimulated clone 5; Transforming growth factor beta-1-induced transcript 1 protein; TSC-5

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Gene Aliases: ARA55; HIC-5; HIC5; TGFB1I1; TSC-5

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UniProt ID: (Human) O43294, (Mouse) Q62219, (Rat) Q99PD6

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Entrez Gene ID: (Human) 7041, (Mouse) 21804, (Rat) 84574

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transcription coactivator activity protein binding zinc ion binding Roundabout binding androgen receptor binding I-SMAD binding metal ion binding

response to heat positive regulation of epithelial to mesenchymal transition Wnt signaling pathway morphogenesis of embryonic epithelium cell differentiation positive regulation of transforming growth factor beta receptor signaling pathway negative regulation of transforming growth factor beta receptor signaling pathway ubiquitin-dependent SMAD protein catabolic process epithelial cell differentiation cell fate commitment negative regulation of fat cell differentiation positive regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell adhesion negative regulation of cell proliferation androgen receptor signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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