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          • Primary Antibodies ›
          • HIF1A Antibodies

          Invitrogen

          HIF-1 alpha Monoclonal Antibody (ESEE122), APC

          View all (92) HIF1A antibodies

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          Cite HIF-1 alpha Monoclonal Antibody (ESEE122), APC

          • Antibody Testing Data (2)
          HIF-1 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          HIF-1 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          HIF-1 alpha Antibody (MA5-45250) in IHC (P)

          Immunohistochemistry analysis using mouse backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with HIF1 alpha monoclonal antibody (Product # MA5-45250) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Membranous and cytoplasmic localization in the epidermis, positive hair follicles, muscle and dermis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          HIF-1 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HIF-1 alpha Antibody in Western Blot (WB)

          Product Details

          MA5-45250

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100
          -

          Immunocytochemistry (ICC/IF)

          1:50
          -

          ELISA (ELISA)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Bovine, Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          ESEE122

          Immunogen

          Recombinant fragment corresponding to amino acids 329-530
          View immunogen

          Conjugate

          APC APC APC
          • FITC
          • PerCP 
          • Request custom conjugation

          Excitation/Emission Max

          651/660 nm View spectra spectra

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          95.64mM phosphate/2.15mM MES, pH 7.4, with 0.5M EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2931704

          Product Specific Information

          1 µg/mL of MA5-45250 was sufficient for detection of HIF1 alpha in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 116kDa. Specific for HIF1Alpha.

          Target Information

          HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: ARNT interacting protein; ARNT-interacting protein; ARNT2; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; hif 1; hif 1a; HIF-1 alpha; HIF-1-alpha; HIF1 alpha; HIF1-alpha; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS protein 1; member of PAS superfamily 1; PAS domain-containing protein 8

          View more View less

          Gene Aliases: AA959795; BHLHE78; BOS_11009; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1A; HIF1alpha; MOP1; PASD8

          View more View less

          UniProt ID: (Human) Q16665, (Mouse) Q61221, (Rat) O35800

          View more View less

          Entrez Gene ID: (Bovine) 281814, (Human) 3091, (Mouse) 15251, (Rat) 29560

          View more View less

          Function(s)
          transcription factor activity, sequence-specific DNA binding signal transducer activity histone acetyltransferase binding transcription regulatory region DNA binding protein dimerization activity transcription factor activity, transcription factor binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding enzyme binding protein kinase binding ubiquitin protein ligase binding protein complex binding nuclear hormone receptor binding histone deacetylase binding sequence-specific DNA binding protein heterodimerization activity Hsp90 protein binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding double-stranded DNA binding
          Process(es)
          response to hypoxia transcription, DNA-templated axonal transport of mitochondrion positive regulation of transcription, DNA-templated cellular response to hypoxia angiogenesis blood vessel development neural crest cell migration epithelial to mesenchymal transition embryonic placenta development B-1 B cell homeostasis vasculature development heart looping positive regulation of neuroblast proliferation connective tissue replacement involved in inflammatory response wound healing outflow tract morphogenesis cardiac ventricle morphogenesis lactate metabolic process regulation of glycolytic process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter cellular iron ion homeostasis signal transduction lactation positive regulation of cell proliferation visual learning regulation of gene expression positive regulation of autophagy vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of epithelial cell migration positive regulation of receptor biosynthetic process response to muscle activity neural fold elevation formation cerebral cortex development cell differentiation negative regulation of bone mineralization positive regulation of vascular endothelial growth factor receptor signaling pathway negative regulation of TOR signaling oxygen homeostasis regulation of transforming growth factor beta2 production collagen metabolic process negative regulation of transcription elongation from RNA polymerase II promoter embryonic hemopoiesis positive regulation of insulin secretion involved in cellular response to glucose stimulus regulation of cell proliferation hemoglobin biosynthetic process glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of neuron apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress positive regulation of erythrocyte differentiation negative regulation of vasoconstriction negative regulation of growth positive regulation of transcription from RNA polymerase II promoter muscle cell cellular homeostasis positive regulation of hormone biosynthetic process blood vessel morphogenesis digestive tract morphogenesis positive regulation of smooth muscle cell proliferation regulation of catalytic activity cartilage development elastin metabolic process intestinal epithelial cell maturation response to fungicide epithelial cell differentiation involved in mammary gland alveolus development retina vasculature development in camera-type eye positive regulation of transcription from RNA polymerase II promoter in response to hypoxia regulation of thymocyte apoptotic process negative regulation of thymocyte apoptotic process dopaminergic neuron differentiation hypoxia-inducible factor-1alpha signaling pathway negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of mitophagy regulation of aerobic respiration negative regulation of reactive oxygen species metabolic process negative regulation of mesenchymal cell apoptotic process negative regulation of transcription from RNA polymerase II promoter positive regulation of endothelial cell proliferation acute-phase response response to salt stress response to X-ray response to auditory stimulus response to purine-containing compound positive regulation of macroautophagy response to estradiol positive regulation of chemokine production cellular response to insulin stimulus cellular response to drug mRNA transcription from RNA polymerase II promoter response to alkaloid response to estrogen positive regulation of angiogenesis positive regulation of cell size positive regulation of glycolytic process positive regulation of nitric-oxide synthase activity response to glucocorticoid maternal process involved in female pregnancy iris morphogenesis regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of chemokine-mediated signaling pathway cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to carbon monoxide cellular response to nitrite cellular response to electrical stimulus cellular response to mechanical stimulus cellular response to cobalt ion cellular response to glucose stimulus cellular response to interleukin-1 cellular response to organic cyclic compound cellular response to light stimulus response to anesthetic cellular response to toxic substance regulation of cellular response to hypoxia positive regulation of pri-miRNA transcription from RNA polymerase II promoter cellular response to cyanide response to mechanical stimulus response to glucose response to activity response to cobalt ion response to drug cellular response to lipid
          It has to be done as per old AB suggested Products section.
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