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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • HIF1A Antibodies

          Invitrogen

          HIF-1 alpha Monoclonal Antibody (Mgc3), APC, eBioscience™

          Advanced Verification
          1 Published Figure
          2 References
          View all (92) HIF1A antibodies

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          Cite HIF-1 alpha Monoclonal Antibody (Mgc3), APC, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (1)
          • Advanced Verification (2)
          HIF-1 alpha Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          HIF-1 alpha Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          HIF-1 alpha Antibody (17-7528-82) in Flow

          HeLa cells were stimulated overnight with 500 µM Deferoxamine (DFOA). Cells were then fixed and permeabilized with 80% methanol, and intracellularly stained with 0.5 µg of Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (blue histogram) or 0.5 µg of HIF1 alpha Monoclonal Antibody, APC (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          HIF-1 alpha Antibody in Flow Cytometry (Flow)
          HIF-1 alpha Antibody in Flow Cytometry (Flow)
          HIF-1 alpha Antibody
          HIF-1 alpha Antibody

          Product Details

          17-7528-82

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          1.0 µg/test
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          Mgc3

          Immunogen

          Human HIF-1 alpha amino acids 530-826.
          View immunogen

          Conjugate

          APC APC APC
          • PE 
          • Request custom conjugation

          Excitation/Emission Max

          651/660 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2802231

          Product Specific Information

          Description: This Mgc3 monoclonal antibody detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with ARNT or the related HIF-2 alpha.

          Mgc3 has been successfully used in western blotting, immunofluorescence, immunocytochemistry, immunoprecipitation, flow cytometry and gel shift procedures. By western blot, this antibody detects a ~93 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells. Immunofluorescence staining of HIF-1 alpha in COS-7 cells with clone Mgc3 yields nuclear staining after exposing cells to 1% oxygen for 4 hours. In gel shift assay experiments, the Mgc3 antibody detected only mouse and not human HIF-1 alpha.

          Other anti-HIF1 alpha antibodies were previously included in a Thermo Scientific Cellomics High Content Screening Kit. Clone Mgc3 is now recommended as a replacement. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. High content assays may require further optimization, including the selection of the optimal Dylight-conjugated secondary antibody.

          Applications Reported: This Mgc3 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This Mgc3 antibody has been tested by flow cytometric analysis of DFOA stimulated HeLa cells using the methanol fixation protocol. This may be used at less than or equal to 1.0 µg/mL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

          Target Information

          HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: ARNT interacting protein; ARNT-interacting protein; ARNT2; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; hif 1; hif 1a; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS protein 1; member of PAS superfamily 1; PAS domain-containing protein 8

          View more View less

          Gene Aliases: AA959795; BHLHE78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1A; HIF1alpha; MOP1; PASD8

          View more View less

          UniProt ID: (Human) Q16665, (Mouse) Q61221

          View more View less

          Entrez Gene ID: (Human) 3091, (Mouse) 15251

          View more View less

          Function(s)
          transcription factor activity, transcription factor binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding enzyme binding protein kinase binding ubiquitin protein ligase binding protein complex binding histone acetyltransferase binding nuclear hormone receptor binding histone deacetylase binding sequence-specific DNA binding protein heterodimerization activity Hsp90 protein binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding signal transducer activity protein dimerization activity
          Process(es)
          angiogenesis blood vessel development response to hypoxia neural crest cell migration epithelial to mesenchymal transition embryonic placenta development B-1 B cell homeostasis vasculature development heart looping positive regulation of neuroblast proliferation connective tissue replacement involved in inflammatory response wound healing outflow tract morphogenesis cardiac ventricle morphogenesis lactate metabolic process regulation of glycolytic process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter cellular iron ion homeostasis signal transduction lactation positive regulation of cell proliferation visual learning regulation of gene expression positive regulation of autophagy vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of epithelial cell migration positive regulation of receptor biosynthetic process response to muscle activity axonal transport of mitochondrion neural fold elevation formation cerebral cortex development cell differentiation negative regulation of bone mineralization positive regulation of vascular endothelial growth factor receptor signaling pathway negative regulation of TOR signaling oxygen homeostasis regulation of transforming growth factor beta2 production collagen metabolic process negative regulation of transcription elongation from RNA polymerase II promoter embryonic hemopoiesis positive regulation of insulin secretion involved in cellular response to glucose stimulus regulation of cell proliferation hemoglobin biosynthetic process glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of neuron apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress positive regulation of erythrocyte differentiation positive regulation of transcription, DNA-templated negative regulation of vasoconstriction negative regulation of growth positive regulation of transcription from RNA polymerase II promoter muscle cell cellular homeostasis positive regulation of hormone biosynthetic process blood vessel morphogenesis digestive tract morphogenesis positive regulation of smooth muscle cell proliferation regulation of catalytic activity cartilage development elastin metabolic process intestinal epithelial cell maturation response to fungicide epithelial cell differentiation involved in mammary gland alveolus development retina vasculature development in camera-type eye positive regulation of transcription from RNA polymerase II promoter in response to hypoxia regulation of thymocyte apoptotic process negative regulation of thymocyte apoptotic process cellular response to hypoxia dopaminergic neuron differentiation hypoxia-inducible factor-1alpha signaling pathway negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of mitophagy regulation of aerobic respiration negative regulation of reactive oxygen species metabolic process negative regulation of mesenchymal cell apoptotic process negative regulation of transcription from RNA polymerase II promoter positive regulation of endothelial cell proliferation acute-phase response response to salt stress response to X-ray response to auditory stimulus response to purine-containing compound positive regulation of macroautophagy response to estradiol positive regulation of chemokine production cellular response to insulin stimulus cellular response to drug mRNA transcription from RNA polymerase II promoter response to alkaloid response to estrogen positive regulation of angiogenesis positive regulation of cell size positive regulation of glycolytic process positive regulation of nitric-oxide synthase activity response to glucocorticoid maternal process involved in female pregnancy iris morphogenesis regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of chemokine-mediated signaling pathway cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to carbon monoxide cellular response to nitrite cellular response to electrical stimulus cellular response to mechanical stimulus cellular response to cobalt ion cellular response to glucose stimulus cellular response to interleukin-1 cellular response to organic cyclic compound cellular response to light stimulus response to anesthetic cellular response to toxic substance regulation of cellular response to hypoxia positive regulation of pri-miRNA transcription from RNA polymerase II promoter cellular response to cyanide
          It has to be done as per old AB suggested Products section.
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