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Invitrogen
HIF-2 alpha Monoclonal Antibody (ep190b)
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FIGURE: 1 / 10
HIF-2 alpha Antibody (MA1-16519) in ICC/IF
Immunofluorescence analysis of HIF-2 alpha was performed using 70% confluent log phase MCF7 treated with Cobalt Chloride (150 µM for 48hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with HIF-2 alpha Monoclonal Antibody (ep190b) (Product # MA1-16519) at... View More
Product Details
MA1-16519
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Hamster,
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
ep190b
Immunogen
Synthetic peptid corresponding to residues 535-631 of human HIF-2 alpha.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein G
Storage buffer
PBS with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2098238
Product Specific Information
Suggested positive control: antigen standard for EPAS1 (transient overexpression lysate).
Target Information
HIF-2 alpha (Epas1) is a transcription factor involved in the induction of genes regulated by oxygen. HIF-2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. It also regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of the lungs. Mutations in this gene are associated with erythrocytosis familial type 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Basic-helix-loop-helix-PAS protein MOP2; bHLHe73; Class E basic helix-loop-helix protein 73; endothelial PAS domain protein 1; Endothelial PAS domain-containing protein 1; EPAS-1; Hif like protein; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2 alpha; HIF-2-alpha; HIF-related factor; HIF1 alpha-like factor; HIF1alpha-like factor; HIF2 alpha; HIF2-alpha; HLF; HLF (HIF1alpha-like factor); HRF; hypoxia inducible factor 2, alpha subunit; hypoxia inducible factor 2a; hypoxia inducible transcription factor 2alpha; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; mHLF; PAS domain-containing protein 2
Gene Aliases: BHLHE73; BOS_11462; ECYT4; EPAS1; HIF-2alpha; HIF2A; HLF; HRF; MOP2; PASD2
UniProt ID: (Human) Q99814, (Mouse) P97481, (Rat) Q9JHS1
Entrez Gene ID: (Bovine) 282711, (Human) 2034, (Mouse) 13819, (Chinese hamster) 100771160, (Rat) 29452
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
protein binding
transcription factor binding
histone acetyltransferase binding
sequence-specific DNA binding
protein heterodimerization activity
transcription factor activity, sequence-specific DNA binding
signal transducer activity
protein dimerization activity
angiogenesis
response to hypoxia
embryonic placenta development
blood vessel remodeling
regulation of heart rate
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
response to oxidative stress
mitochondrion organization
signal transduction
multicellular organism development
visual perception
hemopoiesis
cell differentiation
erythrocyte differentiation
lung development
norepinephrine metabolic process
surfactant homeostasis
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
positive regulation of transcription from RNA polymerase II promoter
cell maturation
myoblast fate commitment
iron ion homeostasis
cellular response to hypoxia
somatic stem cell population maintenance
regulation of transcription from RNA polymerase II promoter in response to hypoxia
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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