Invitrogen

HIF1A Monoclonal Antibody (mgc3)

HIF1A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 28

HIF1A Antibody (MA1-516) in ICC/IF

Immunofluorescent analysis of HIF-1 alpha (green) in HeLa cells either left untreated (left panel) or treated with 100uM Deferoxamine mesylate for ~16 hours (right panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were probed with a HIF-1 alpha monoclonal antibody (Product # MA1-516) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat... View More

HIF1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HIF1A Antibody in Immunohistochemistry (IHC)

Product Image Gallery

HIF1A Antibody — The specificity of anti-HIF1-alpha antibody (Product # MA1-516) was demonstrated by the immunofluorescence detection of increased accumulation of HIF1-alpha in the nucleus in HeLa cells upon treatment with deferoxamine mesylate (right panel) compared to untreated cells (left panel). {TM}

Product Details

MA1-516

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Neutralization (Neu)

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

View 1 publication 1 publication

Immunoelectrophoresis (IE)

Assay-dependent

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Non-human primate, Pig

Published species

Bovine, Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

mgc3

Immunogen

Human HIF-1 alpha amino acids 530-826.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325431

Product Specific Information

MA1-516 detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with ARNT or the related HIF-2 alpha.

MA1-516 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, immunocytochemistry, and gel shift procedures. By Western blot, this antibody detects an ~116 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells. Immunofluorescence staining of HIF-1 alpha in COS-7 cells with MA1-516 yields nuclear staining after exposing cells to 1% oxygen for 4 hours. In gel shift assays, MA1-516 has been successfully used only with mouse HIF-1 alpha.

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

Target Information

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Low-Level Laser Therapy Induces Melanoma Tumor Growth by Promoting Angiogenesis.

Life (Basel, Switzerland)

Lin YY,Lee SY,Cheng YJ

MA1-516 was used in Gel Shift, Immunocytochemistry, Immunoelectrophoresis, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to indicate that LLLT induces melanoma tumor growth by promoting angiogenesis.

Mon Jan 23 00:00:00 UTC 2023

Low-Level Laser Therapy Induces Melanoma Tumor Growth by Promoting Angiogenesis.

Life (Basel, Switzerland)

Lin YY,Lee SY,Cheng YJ

Mon Jan 23 00:00:00 UTC 2023

Activating transcription factor 4 regulates hypoxia inducible factor 1α in chronic hypoxia in pancreatic cancer cells.

Oncology reports

Chee NT,Carriere CH,Miller Z,Welford S,Brothers SP

MA1-516 was used in Gel Shift, Immunocytochemistry, Immunoelectrophoresis, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to demonstrate differential response of ATF4 in chronic hypoxia compared with acute hypoxia, and implicate this response via interaction with HIF proteins in PDAC.

Sun Jan 01 00:00:00 UTC 2023

Activating transcription factor 4 regulates hypoxia inducible factor 1α in chronic hypoxia in pancreatic cancer cells.

Oncology reports

Chee NT,Carriere CH,Miller Z,Welford S,Brothers SP

Sun Jan 01 00:00:00 UTC 2023

Antiangiogenic Action of JZL184 on Endothelial Cells via Inhibition of VEGF Expression in Hypoxic Lung Cancer Cells.

Cells

Wittig F,Pannenberg L,Schwarz R,Bekeschus S,Ramer R,Hinz B

Published figure using HIF1A monoclonal antibody (Product # MA1-516) in Western Blot

Fri Sep 22 00:00:00 UTC 2023

Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model.

Experimental & molecular medicine

Lee AR,Lee SY,Choi JW,Um IG,Na HS,Lee JH,Cho ML

MA1-516 was used in Immunohistochemistry to develop the patient-derived keloid xenograft (PDKX), which is a humanized mouse model, and compared it to the traditional mouse xenograft model (transplantation of only keloid lesions).

Tue Aug 01 00:00:00 UTC 2023

IL-17 Induces Autophagy Dysfunction to Promote Inflammatory Cell Death and Fibrosis in Keloid Fibroblasts via the STAT3 and HIF-1α Dependent Signaling Pathways.

Frontiers in immunology

Lee SY,Lee AR,Choi JW,Lee CR,Cho KH,Lee JH,Cho ML

Published figure using HIF1A monoclonal antibody (Product # MA1-516) in Immunohistochemistry

Tue Jun 28 00:00:00 UTC 2022

p53N236S Activates Autophagy in Response to Hypoxic Stress Induced by DFO.

Genes

Gao K,Zong H,Hou K,Zhang Y,Zhang R,Zhao D,Guo X,Luo Y,Jia S

MA1-516 was used in Western Blotting to show that the p53S mutant activates autophagy instead of inducing an apoptotic process in response to hypoxia stress to protect cells from death.

Tue Apr 26 00:00:00 UTC 2022

Untangling Galectin-Mediated Circuits that Control Hypoxia-Driven Angiogenesis.

Methods in molecular biology (Clifton, N.J.)

Bannoud N,García PA,Gambarte-Tudela J,Sundblad V,Cagnoni AJ,Bach CA,Pérez Saez JM,Blidner AG,Maller SM,Mariño KV,Salatino M,Cerliani JP,Rabinovich GA,Croci DO

MA1-516 was used in Gel Shift, Immunocytochemistry, Immunoelectrophoresis, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Flow Cytometry to describe selected strategies and methods used to study the role of hypoxia-regulated galectins in the regulation of blood vessel formation.

Fri Mar 25 00:00:00 UTC 2022

Untangling Galectin-Mediated Circuits that Control Hypoxia-Driven Angiogenesis.

Methods in molecular biology (Clifton, N.J.)

Bannoud N,García PA,Gambarte-Tudela J,Sundblad V,Cagnoni AJ,Bach CA,Pérez Saez JM,Blidner AG,Maller SM,Mariño KV,Salatino M,Cerliani JP,Rabinovich GA,Croci DO

Fri Mar 25 00:00:00 UTC 2022

Physiological Effects of Intermittent Passive Exposure to Hypobaric Hypoxia and Cold in Rats.

Frontiers in physiology

Santocildes G,Viscor G,Pagès T,Ramos-Romero S,Torres JL,Torrella JR

MA1-516 was used in Western Blot to study the cellular response to simultaneous intermittent exposure to hypobaric hypoxia and cold.

Sun Aug 11 00:00:00 UTC 2024

Physiological Effects of Intermittent Passive Exposure to Hypobaric Hypoxia and Cold in Rats.

Frontiers in physiology

Santocildes G,Viscor G,Pagès T,Ramos-Romero S,Torres JL,Torrella JR

MA1-516 was used in Western Blot to study the cellular response to simultaneous intermittent exposure to hypobaric hypoxia and cold.

Sun Aug 11 00:00:00 UTC 2024

Arginine is neuroprotective through suppressing HIF-1α/LDHA-mediated inflammatory response after cerebral ischemia/reperfusion injury.

Molecular brain

Chen SF,Pan MX,Tang JC,Cheng J,Zhao D,Zhang Y,Liao HB,Liu R,Zhuang Y,Zhang ZF,Chen J,Lei RX,Li SF,Li HT,Wang ZF,Wan Q

MA1-516 was used in Western Blotting to indicate a possibility that arginine-induced neuroprotective effect may be through the suppression of HIF-1α/LDHA-mediated inflammatory response in microglia after cerebral ischemia injury.

Wed Apr 22 00:00:00 UTC 2020

Arginine is neuroprotective through suppressing HIF-1α/LDHA-mediated inflammatory response after cerebral ischemia/reperfusion injury.

Molecular brain

Chen SF,Pan MX,Tang JC,Cheng J,Zhao D,Zhang Y,Liao HB,Liu R,Zhuang Y,Zhang ZF,Chen J,Lei RX,Li SF,Li HT,Wang ZF,Wan Q

Wed Apr 22 00:00:00 UTC 2020

The HIF1α/JMY pathway promotes glioblastoma stem-like cell invasiveness after irradiation.

Scientific reports

Gauthier LR,Saati M,Bensalah-Pigeon H,Ben M'Barek K,Gitton-Quent O,Bertrand R,Busso D,Mouthon MA,Collura A,Junier MP,Chneiweiss H,Pineda JR,Boussin FD

MA1-516 was used in Immunocytochemistry-immunoflourescence to show that ionizing radiation enhances the motility and invasiveness of human glioblastomas through the HIF1α/JMY pathway.

Fri Oct 30 00:00:00 UTC 2020

Flow-Encoded Oxygen Control to Track the Time-Dependence of Molecular Changes Induced by Static or Cycling Hypoxia.

Analytical chemistry

Yao M,Rabbani ZN,Sattler T,Nguyen KG,Zaharoff DA,Walker G,Gamcsik MP

MA1-516 was used in Immunohistochemistry to show how a manifold and plate design can be used to expose cells to either static or cycling hypoxic conditions for eight different time periods thereby facilitating the study of the time-response of cells to altered oxygen environments.

Tue Dec 03 00:00:00 UTC 2019

Shmt2: A Stat3 Signaling New Player in Prostate Cancer Energy Metabolism.

Cells

Marrocco I,Altieri F,Rubini E,Paglia G,Chichiarelli S,Giamogante F,Macone A,Perugia G,Magliocca FM,Gurtner A,Maras B,Ragno R,Patsilinakos A,Manganaro R,Eufemi M

MA1-516 was used in Western Blotting to demonstrate that in LNCaP, prostate cancer cells' SHMT2 expression is upregulated by the JAK2/STAT3 canonical pathway upon IL-6 stimulation.

Fri Sep 06 00:00:00 UTC 2019

Molecular imaging HDACs class IIa expression-activity and pharmacologic inhibition in intracerebral glioma models in rats using PET/CT/(MRI) with [¹⁸F]TFAHA.

Scientific reports

Laws MT,Bonomi RE,Kamal S,Gelovani DJ,Llaniguez J,Potukutchi S,Lu X,Mangner T,Gelovani JG

MA1-516 was used in Immunohistochemistry to demonstrate the efficacy of PET/CT/(MRI) with [18F]TFAHA for non-invasive and quantitative imaging of HDAC class IIa expression-activity in intracerebral 9L and U87-MG gliomas in rats.

Tue Mar 05 00:00:00 UTC 2019

Innate immune memory in the brain shapes neurological disease hallmarks.

Nature

Wendeln AC,Degenhardt K,Kaurani L,Gertig M,Ulas T,Jain G,Wagner J,Häsler LM,Wild K,Skodras A,Blank T,Staszewski O,Datta M,Centeno TP,Capece V,Islam MR,Kerimoglu C,Staufenbiel M,Schultze JL,Beyer M,Prinz M,Jucker M,Fischer A,Neher JJ

MA1-516 was used in Immunohistochemistry-immunofluorescence to identify immune memory in the brain as an important modifier of neuropathology.

Sun Apr 01 00:00:00 UTC 2018

An Integrated Score and Nomogram Combining Clinical and Immunohistochemistry Factors to Predict High ISUP Grade Clear Cell Renal Cell Carcinoma.

Frontiers in oncology

Wu J,Xu WH,Wei Y,Qu YY,Zhang HL,Ye DW

MA1-516 was used in Immunohistochemistry to combine clinical and immunohistochemical factors for the prediction of high ISUP grade clear cell renal cell carcinoma in an attempt to improve the accuracy of a biopsy-based diagnosis.

Thu Oct 01 00:00:00 UTC 2020

Mixing and delivery of multiple controlled oxygen environments to a single multiwell culture plate.

American journal of physiology. Cell physiology

Yao M,Sattler T,Rabbani ZN,Pulliam T,Walker G,Gamcsik MP

MA1-516 was used in Immunohistochemistry-immunofluorescence to demonstrate a design which is capable of mixing and delivering differing oxygen environments to separate rows of a single multiwell culture plate.

Thu Nov 01 00:00:00 UTC 2018

Mixing and delivery of multiple controlled oxygen environments to a single multiwell culture plate.

American journal of physiology. Cell physiology

Yao M,Sattler T,Rabbani ZN,Pulliam T,Walker G,Gamcsik MP

Thu Nov 01 00:00:00 UTC 2018

STAT3, a Hub Protein of Cellular Signaling Pathways, Is Triggered by β-Hexaclorocyclohexane.

International journal of molecular sciences

Rubini E,Altieri F,Chichiarelli S,Giamogante F,Carissimi S,Paglia G,Macone A,Eufemi M

Published figure using HIF1A monoclonal antibody (Product # MA1-516) in Western Blot

Fri Jul 20 00:00:00 UTC 2018

Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death.

Scientific reports

Omatsu-Kanbe M,Nozuchi N,Nishino Y,Mukaisho KI,Sugihara H,Matsuura H

MA1516 was used in immunocytochemistry to ask whether PrP+ cTnT+ cells are present in the human heart tissue with myocardial infarction

Wed Jan 25 00:00:00 UTC 2017

pVHL-mediated degradation of HIF-2α regulates estrogen receptor α expression in normoxic breast cancer cells.

FEBS letters

Higashimura Y,Kitakaze T,Harada N,Inui H,Nakano Y,Yamaji R

MA1516 was used in western blot to propose that HIF-2-alpha is a negative regulator of estrogen receptor expression in breast cancer

Mon Aug 01 00:00:00 UTC 2016

A common functional regulatory variant at a type 2 diabetes locus upregulates ARAP1 expression in the pancreatic beta cell.

American journal of human genetics

Kulzer JR,Stitzel ML,Morken MA,Huyghe JR,Fuchsberger C,Kuusisto J,Laakso M,Boehnke M,Collins FS,Mohlke KL

MA1-516 was used in EMSA to study the upregulation of pancreatic beta cell ARAP1 by a functional regulatory DNA variant at a type 2 diabetes locus

Thu Feb 06 00:00:00 UTC 2014

STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy.

Autophagy

Ferreira JV,Fôfo H,Bejarano E,Bento CF,Ramalho JS,Girão H,Pereira P

MA1-516 was used in Immunohistochemistry to show that HIF1A can be targeted for degradation in the lysosome by CMA and this targeting is dependent on its interaction with the ubiquitin ligase STUB1.

Sun Sep 01 00:00:00 UTC 2013

STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy.

Autophagy

Ferreira JV,Fôfo H,Bejarano E,Bento CF,Ramalho JS,Girão H,Pereira P

MA1-516 was used in Immunohistochemistry to show that HIF1A can be targeted for degradation in the lysosome by CMA and this targeting is dependent on its interaction with the ubiquitin ligase STUB1.

Sun Sep 01 00:00:00 UTC 2013

Human hair follicle stem/progenitor cells express hypoxia markers.

The Journal of investigative dermatology

Rathman-Josserand M,Genty G,Lecardonnel J,Chabane S,Cousson A,François Michelet J,Bernard BA

MA1-516 was used in Western Blotting to show that human hair follicles harbour two stem cell compartments that can be distinguished by differential protein expression and microenvironment.

Thu Aug 01 00:00:00 UTC 2013

Hypoxia-inducible factor and vascular endothelial growth factor are targets of dietary soy during acute stroke in female rats.

Endocrinology

Ma Y,Lovekamp-Swan T,Bekele W,Dohi A,Schreihofer DA

MA1-516 was used in western blot to study the role of HIF-1alpha and VEGF in the neuroprotective effects of soy following acute stroke

Mon Apr 01 00:00:00 UTC 2013

Soluble FLT1 binds lipid microdomains in podocytes to control cell morphology and glomerular barrier function.

Cell

Jin J,Sison K,Li C,Tian R,Wnuk M,Sung HK,Jeansson M,Zhang C,Tucholska M,Jones N,Kerjaschki D,Shibuya M,Fantus IG,Nagy A,Gerber HP,Ferrara N,Pawson T,Quaggin SE

MA1-516 was used in blocking or activating experiment to study the role of the soluble isoform of FLT-1 in modulating podocyte/pericyte function

Fri Oct 12 00:00:00 UTC 2012

Disrupting galectin-1 interactions with N-glycans suppresses hypoxia-driven angiogenesis and tumorigenesis in Kaposi's sarcoma.

The Journal of experimental medicine

Croci DO,Salatino M,Rubinstein N,Cerliani JP,Cavallin LE,Leung HJ,Ouyang J,Ilarregui JM,Toscano MA,Domaica CI,Croci MC,Shipp MA,Mesri EA,Albini A,Rabinovich GA

MA1-516 was used in western blot to study the role of galectin-1 interactions with N-glycans in hypoxia-mediated angiogenesis and tumorigenesis in Kaposi's sarcoma

Mon Oct 22 00:00:00 UTC 2012

Disrupting galectin-1 interactions with N-glycans suppresses hypoxia-driven angiogenesis and tumorigenesis in Kaposi's sarcoma.

The Journal of experimental medicine

Croci DO,Salatino M,Rubinstein N,Cerliani JP,Cavallin LE,Leung HJ,Ouyang J,Ilarregui JM,Toscano MA,Domaica CI,Croci MC,Shipp MA,Mesri EA,Albini A,Rabinovich GA

MA1-516 was used in western blot to study the role of galectin-1 interactions with N-glycans in hypoxia-mediated angiogenesis and tumorigenesis in Kaposi's sarcoma

Mon Oct 22 00:00:00 UTC 2012

Up-regulation of glyceraldehyde-3-phosphate dehydrogenase gene expression by HIF-1 activity depending on Sp1 in hypoxic breast cancer cells.

Archives of biochemistry and biophysics

Higashimura Y,Nakajima Y,Yamaji R,Harada N,Shibasaki F,Nakano Y,Inui H

MA1-516 was used in western blot to investigate the effect of HIF-1 on the regulation of GAPDH expression in hypoxic breast cancer cells

Sun May 01 00:00:00 UTC 2011

Hypoxia preconditioning protection of corneal stromal cells requires HIF1alpha but not VEGF.

Molecular vision

Xing D,Bonanno JA

MA1-516 was used in western blot to investigate the role of HIF-1alpha on hypoxia preconditioning protection in corneal stromal cells

Mon May 18 00:00:00 UTC 2009

Hypoxia upregulates carcinoembryonic antigen expression in cancer cells.

International journal of cancer

Kokkonen N,Ulibarri IF,Kauppila A,Luosujärvi H,Rivinoja A,Pospiech H,Kellokumpu I,Kellokumpu S

MA1-516 was used in western blot to study the upregulation of carcinoembryonic antigen expression in cancer cells by hypoxia

Sat Dec 01 00:00:00 UTC 2007

Hypoxia preconditioning protects corneal stromal cells against induced apoptosis.

Experimental eye research

Xing D,Sun X,Li J,Cui M,Tan-Allen K,Bonanno JA

MA1-516 was used in western blot to investigate the influence of hypoxia on corneal stromal cell survival

Mon May 01 00:00:00 UTC 2006

Role of oxygen and vascular development in epithelial branching morphogenesis of the developing mouse lung.

American journal of physiology. Lung cellular and molecular physiology

van Tuyl M,Liu J,Wang J,Kuliszewski M,Tibboel D,Post M

MA1-516 was used in western blot to study the effect of oxygen level during mouse lung development

Sat Jan 01 00:00:00 UTC 2005

Experimental angiogenesis of arterial vasa vasorum.

Cell and tissue research

Bayer IM,Caniggia I,Adamson SL,Langille BL

MA1-516 was used in immunohistochemistry to study the development of arterial vasa vasorum in a rabbit model

Fri Mar 01 00:00:00 UTC 2002

Angiogenic potential of prostate carcinoma cells overexpressing bcl-2.

Journal of the National Cancer Institute

Fernandez A,Udagawa T,Schwesinger C,Beecken W,Achilles-Gerte E,McDonnell T,D'Amato R

MA1-516 was used in western blot to investigate the role of bcl-2 in tumorigenesis

Wed Feb 07 00:00:00 UTC 2001

Bioinformatics

Protein Aliases: ARNT interacting protein; ARNT-interacting protein; ARNT2; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; hif 1; hif 1a; HIF-1 alpha; HIF-1-alpha; HIF1 alpha; HIF1-alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS protein 1; member of PAS superfamily 1; PAS domain-containing protein 8

View more View less

Gene Aliases: AA959795; BHLHE78; BOS_11009; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1A; HIF1alpha; MOP1; PASD8

View more View less

UniProt ID: (Human) Q16665, (Mouse) Q61221

View more View less

Entrez Gene ID: (Bovine) 281814, (Pig) 396696, (Human) 3091, (Mouse) 15251

View more View less

Function(s)

transcription factor activity, sequence-specific DNA binding signal transducer activity histone acetyltransferase binding transcription regulatory region DNA binding protein dimerization activity transcription factor activity, transcription factor binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding enzyme binding protein kinase binding ubiquitin protein ligase binding protein complex binding nuclear hormone receptor binding histone deacetylase binding sequence-specific DNA binding protein heterodimerization activity Hsp90 protein binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

HIF1A Monoclonal Antibody (mgc3)

HIF1A Antibody (MA1-516) in ICC/IF

Product Details

MA1-516

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Gel Shift (GS)

Immunoelectrophoresis (IE)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!