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HJURP Polyclonal Antibody

HJURP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HJURP Antibody (15283-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human brain tissue slide using 15283-1-AP ( HJURP antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

15283-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HJURP Fusion Protein Ag7402 (394-748 aa encoded by BC001940)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ATYNLDEEN RFRTLKWLIS PVKIVSRPTI RQGHGENRQR EIEIRFDQLH REYCLSPRNQ PRRMCLPDSW AMNMYRGGPA SPGGLQGLET RRLSLPSSKA KAKSLSEAFE NLGKRSLEAG RCLPKSDSSS SLPKTNPTHS ATRPQQTSDL HVQGNSSGIF RKSVSPSKTL SVPDKEVPGH GRNRYDEIKE EFDKLHQKYC LKSPGQMTVP LCIGVSTDKA SMEVRYQTEG FLGKLNPDPH FQGFQKLPSS PLGCRKSLLG STAIEAPSST CVARAITRDG TRDHQFPAKR PRLSEPQGSG RQGNSLGASD GVDNTVRPGD QGSSSQPNSE ERGENTSYRM EEKSDFMLEK LETKSV (394-748 aa encoded by BC001940)

Target Information

Holliday junction recognition protein (HJURP) is a centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. It acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. HJURP prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. It directly binds to Holliday junctions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CENP-I directly targets centromeric DNA to support CENP-A deposition and centromere maintenance.

Proceedings of the National Academy of Sciences of the United States of America

Hu L,Zhao C,Liu M,Liu S,Ye J,Wang K,Shi J,Tian W,He X

Tue Mar 14 00:00:00 UTC 2023

Hypomethylation-driven overexpression of HJURP promotes progression of hepatocellular carcinoma and is associated with poor prognosis.

Biochemical and biophysical research communications

Li Y,Yi Q,Liao X,Han C,Zheng L,Li H,Yu Q,Yan X,Chen X,Zhu H,Zhao B,Lin Q,Liang L,Wang L,Qin F,Xie W,Li Y,Huang W

Fri Aug 20 00:00:00 UTC 2021

Comprehensive Analysis Uncovers Prognostic and Immunogenic Characteristics of Cellular Senescence for Lung Adenocarcinoma.

Frontiers in cell and developmental biology

Lin W,Wang X,Wang Z,Shao F,Yang Y,Cao Z,Feng X,Gao Y,He J

Tue Dec 07 00:00:00 UTC 2021

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A).

Journal of visualized experiments : JoVE

Niikura Y,Fang L,Kitagawa R,Li P,Xi Y,You J,Guo Y,Kitagawa K

Wed Jun 10 00:00:00 UTC 2020

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

International journal of cancer

Fu F,Zhang Y,Gao Z,Zhao Y,Wen Z,Han H,Li Y,Chen H

Wed Jul 15 00:00:00 UTC 2020

CENP-A Ubiquitylation Is Indispensable to Cell Viability.

Developmental cell

Niikura Y,Kitagawa R,Fang L,Kitagawa K

Mon Sep 23 00:00:00 UTC 2019

Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone holliday junction recognition protein (HJURP).

The Journal of biological chemistry

Wang J,Liu X,Dou Z,Chen L,Jiang H,Fu C,Fu G,Liu D,Zhang J,Zhu T,Fang J,Zang J,Cheng J,Teng M,Ding X,Yao X

Fri Mar 21 00:00:00 UTC 2014

Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone holliday junction recognition protein (HJURP).

The Journal of biological chemistry

Wang J,Liu X,Dou Z,Chen L,Jiang H,Fu C,Fu G,Liu D,Zhang J,Zhu T,Fang J,Zang J,Cheng J,Teng M,Ding X,Yao X

Fri Mar 21 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: 14-3-3-associated AKT substrate; fetal liver expressing gene 1; Fetal liver-expressing gene 1 protein; Holliday junction recognition protein; Up-regulated in lung cancer 9

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Gene Aliases: FAKTS; FLEG1; hFLEG1; HJURP; URLC9

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UniProt ID: (Human) Q8NCD3

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Entrez Gene ID: (Human) 55355

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Function(s)

DNA binding protein binding histone binding identical protein binding

Process(es)

cell cycle chromosome segregation CENP-A containing nucleosome assembly regulation of protein complex assembly regulation of DNA binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HJURP Polyclonal Antibody

HJURP Antibody (15283-1-AP) in IHC (P)

Product Details

15283-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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