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Invitrogen
HJURP Recombinant Rabbit Monoclonal Antibody (16H6L17)
FIGURE: 1 / 6
HJURP Antibody (703460) in ICC/IF
Knockdown of HJURP was achieved by transfecting A549 cells with specific siRNA (Silencer® select Product # s30814; s30813). Immunofluorescence analysis was performed on A549 cells (untransfected, panel a-d), transfected with HJURP specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labelled with Anti-HJURP Recombinant Rabbit Monoclonal Antibody (Product # 703460, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjuga... View More
Product Details
703460
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
16H6L17
Immunogen
Proteins corresponding to human HJURP [1) aa47-aa177 2)aa394-aa748]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809238
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Cat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Holliday junction recognition protein (HJURP) is a centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. It acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. HJURP prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. It directly binds to Holliday junctions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14-3-3-associated AKT substrate; fetal liver expressing gene 1; Fetal liver-expressing gene 1 protein; Holliday junction recognition protein; Up-regulated in lung cancer 9
Gene Aliases: FAKTS; FLEG1; hFLEG1; HJURP; URLC9
UniProt ID: (Human) Q8NCD3
Entrez Gene ID: (Human) 55355
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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