Invitrogen

HLA-A2 Monoclonal Antibody (BB7.2), APC, eBioscience™

HLA-A2 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 6

HLA-A2 Antibody (17-9876-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (blue histogram) or Anti-Human HLA-A2 APC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-9876-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

BB7.2

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11149299

Product Specific Information

Description: This BB7.2 monoclonal antibody recognizes the human major histocompatibility complex (MHC) class I molecule HLA-A2, which is a member of the HLA-A family. MHC class I exists as a single alpha chain composed of three domains. This molecule associates with beta 2-microglobulin and is expressed on all nucleated cells.

The BB7.2 monoclonal antibody has been reported to exhibit blocking activity, as well as recognize HLA-A28.

Applications Reported: This BB7.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This BB7.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.

Immunity

Jongsma MLM,de Waard AA,Raaben M,Zhang T,Cabukusta B,Platzer R,Blomen VA,Xagara A,Verkerk T,Bliss S,Kong X,Gerke C,Janssen L,Stickel E,Holst S,Plomp R,Mulder A,Ferrone S,Claas FHJ,Heemskerk MHM,Griffioen M,Halenius A,Overkleeft H,Huppa JB,Wuhrer M,Brummelkamp TR,Neefjes J,Spaapen RM

17-9876-42 was used in Flow Cytometry to identify a GSL signature that inhibits immune recognition and represents a potential therapeutic target in cancer, infection, and autoimmunity.

Tue Jan 12 00:00:00 UTC 2021

Single-cell transcriptome analysis of the heterogeneous effects of differential expression of tumor PD-L1 on responding TCR-T cells.

Theranostics

Ding R,Liu S,Wang S,Chen H,Wang F,Xu Q,Zhu L,Dong X,Gu Y,Zhang X,Chao CC,Gao Q

17-9876-42 was used in Flow Cytometry to evaluate how tumor expression of PD-L1 affects T cell functionality in anti-cancer TCR-T cell therapy.

Mon Aug 02 00:00:00 UTC 2021

Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells.

JCI insight

Dawson NA,Lamarche C,Hoeppli RE,Bergqvist P,Fung VC,McIver E,Huang Q,Gillies J,Speck M,Orban PC,Bush JW,Mojibian M,Levings MK

17-9876 was used in Flow cytometry/Cell sorting to characterise and test alloantigen-specific chimeric antigen receptors in regulatory T cells.

Thu Mar 21 00:00:00 UTC 2019

A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens.

The Journal of clinical investigation

Chang AY,Dao T,Gejman RS,Jarvis CA,Scott A,Dubrovsky L,Mathias MD,Korontsvit T,Zakhaleva V,Curcio M,Hendrickson RC,Liu C,Scheinberg DA

17-9876 was used in Flow cytometry/Cell sorting to investigate the effect of Pr20 on cancer.

Fri Jun 30 00:00:00 UTC 2017

Expression Profiles of Ligands for Activating Natural Killer Cell Receptors on HIV Infected and Uninfected CD4⁺ T Cells.

Viruses

Tremblay-McLean A,Bruneau J,Lebouché B,Lisovsky I,Song R,Bernard NF

17-9876 was used in Flow cytometry/Cell sorting to characterise the expression profiles of ligands for activating natural killer cell receptors on HIV infected and uninfected CD4⁺ T cells.

Thu Oct 12 00:00:00 UTC 2017

Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand.

PLoS pathogens

Tomić A,Varanasi PR,Golemac M,Malić S,Riese P,Borst EM,Mischak-Weissinger E,Guzmán CA,Krmpotić A,Jonjić S,Messerle M

17-9876 was used in Flow cytometry/Cell sorting to investigate a strategy for an HCMV vaccine that aims at the simultaneous activation of innate and adaptive immune responses.

Thu Dec 01 00:00:00 UTC 2016

Antibody stabilization of peptide-MHC multimers reveals functional T cells bearing extremely low-affinity TCRs.

Journal of immunology (Baltimore, Md. : 1950)

Tungatt K,Bianchi V,Crowther MD,Powell WE,Schauenburg AJ,Trimby A,Donia M,Miles JJ,Holland CJ,Cole DK,Godkin AJ,Peakman M,Straten PT,Svane IM,Sewell AK,Dolton G

17-9876 was used in Flow cytometry to conclude Ab stabilization of pMHC multimers during T cell staining extends the range of TCR affinities that can be detected, yields considerably enhanced staining intensities, and is compatible with using reduced amounts of these expensive reagents.

Thu Jan 01 00:00:00 UTC 2015

A Novel Method Linking Antigen Presentation by Human Monocyte-Derived Macrophages to CD8(+) T Cell Polyfunctionality.

Frontiers in immunology

Short KR,Grant EJ,Vissers M,Reading PC,Diavatopoulos DA,Kedzierska K

17-9876 was used in Flow cytometry/Cell sorting to study how virally infected macrophages impact T cell function.

Fri Dec 06 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: A29 protein; antigen presenting molecule; DAQB-90C11.16; FLJ26655; HLA A; HLA class I antigen; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-30 alpha chain; HLA class I histocompatibility antigen, A-33 alpha chain; HLA class I histocompatibility antigen, A-74 alpha chain; HLA locus; HLA-A; HLA-A locus alpha 2 domain; HLA-A null; HLA-Aw33.1; Human leukocyte antigen A; leucocyte antigen A; leukocyte antigen class I-A; MCH class I antigen; MHC 1; MHC class 1 antigen; MHC class I anti; MHC Class I antigen; MHC class I antigen A*30; MHC class I antigen A*33; MHC class I antigen A*74; MHC class I antigen heavy chain; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-A33; MHC class I antigen null protein; MHC class I antigene; MHC class II antigen; MHC classI antigen; MHC1; truncated MHC class I antigen

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Gene Aliases: Aw-33; Aw-74; HLA-A; HLA-A11; HLA-A33; HLA-DQB1; HLA-DRB1; HLAA

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UniProt ID: (Human) P04439

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Entrez Gene ID: (Human) 3105

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Function(s)

protein binding beta-2-microglobulin binding peptide antigen binding poly(A) RNA binding

Process(es)

antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent viral process antigen processing and presentation regulation of immune response interferon-gamma-mediated signaling pathway type I interferon signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HLA-A2 Monoclonal Antibody (BB7.2), APC, eBioscience™

HLA-A2 Antibody (17-9876-42) in Flow

Product Details

17-9876-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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