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Leinco Technologies
HLA-DQ (MHC Class II) Monoclonal Antibody (1a3), Functional Grade, In Vivo GOLD™
Product Details
H262-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
1a3
Immunogen
Proprietary immunogen
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.2-7.4
Contains
no preservative
Storage conditions
4°C short term, -80°C long term
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Specificity: Clone 1a3 recognizes a monomorphic epitope on human HLA-DQ1. It does not cross-react with HLA-DR or HLA-DP.
Endotoxin Level: ≤ 1.0 EU/mg as determined by the LAL method. Purity: >95% by SDS Page; ≥95% monomer by analytical SEC.
Product Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8 C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80 C. Avoid Repeated Freeze Thaw Cycles.
This antibody has been tested in the following applications: Western Blotting, Immunoprecipitation, Flow Cytometry, ELISA.
Target Information
MHC class II is constitutively expressed on human professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation. HLA-DQ consists of two transmembrane proteins, a 35 kDa alpha (heavy) chain and 29 kDa Beta (light) chain 3 encoded by the HLA-DQA1 and HLA-DQB1 genes, respectively, located in the HLA complex of chromosome 6. The N-terminal alpha-1 and Beta-1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation and can transduce signals into T cells, enhancing T cell proliferation and activity. Specific alleles of HLA-DQ are associated with autoimmune diseases, including celiac disease and type 1 diabetes, and graft-versus-host disease.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DC-1 alpha chain; DC-alpha; HLA class II antigen; HLA class II histocompatibility antigen, DQ alpha 1 chain; HLA class II histocompatibility antigen, DQ beta 1 chain; HLA class II histocompatibility antigen, DQ(W3) alpha chain; HLA-DCA; leucocyte antigen DQA1; leukocyte antigen alpha chain; lymphocyte antigen; MHC class II antigen; MHC class II antigen DQB1; MHC class II antigen HLA-DQ-beta-1; MHC class II DQ beta chain; MHC class II DQA1; MHC class II HLA-D alpha glycoprotein; MHC class II HLA-DQ beta glycoprotein; MHC class II HLA-DQ-alpha-1; MHC class II surface glycoprotein; MHC class2 antigen; MHC DQ beta; MHC HLA-DQ alpha
Gene Aliases: CD; CELIAC1; DQ-A1; GSE; HLA-DQA; HLA-DQA1; HLA-DQB; HLA-DQB1; HLA-DRB1; IDDM1
UniProt ID: (Human) P01909, (Human) P01920
Entrez Gene ID: (Human) 3117, (Human) 3119
immunoglobulin production involved in immunoglobulin mediated immune response
humoral immune response mediated by circulating immunoglobulin
immune response
antigen processing and presentation of exogenous peptide antigen via MHC class II
T cell costimulation
T cell receptor signaling pathway
interferon-gamma-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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