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Product Details
MA5-16502
Product Specifications
Species Reactivity
Bovine,
Goat,
Human,
Mouse,
Sheep
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
37.68
Immunogen
Splenocytes from ATL mice (1)
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538013
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BoLA-DR-alpha; BoLA-DRB3.2; CD74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; class II-associated invariant chain peptide; DASS-397D15.1; dinucleotide microsatellite; DR beta-5; DR beta-chain antigen binding domain; DR-9; DR-beta chain MHC class II; DR2-beta-2; DR7; DR9; DRB1 transplantation antigen; Dw2; DW2.2/DR2.2; FLJ51114; FLJ75017; FLJ76359; gamma chain of class II antigens; H-2 class II histocompatibility antigen gamma chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H2-IE-alpha; histocompatibility 2, class II antigen E alpha; histocompatibility antigen HLA-DR alpha; histocompatibility complex, class II, DR beta 3; histocompatibility: class II antigens, gamma chain of; HLA class II histocompatibility antigen gamma chain; HLA class II histocompatibility antigen, DR alpha chain; HLA class II histocompatibility antigen, DR beta 3 chain; HLA class II histocompatibility antigen, DR beta 4 chain; HLA class II histocompatibility antigen, DR beta 5 chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DR-5 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA class II histocompatibility antigen, DRB1-7 beta chain; HLA class II histocompatibility antigen, DRB1-9 beta chain; HLA-DR antigens-associated invariant chain; HLA-DR-GAMMA; HLA-DRB1; HLA-DRB5; human leucocyte antigen DRB1; human leucocyte antigen DRB3; human leucocyte antigen DRB4; human leucocyte antigen DRB5; Human leukocyte antigen DRB1; I-E alpha MHC class II; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ii; invariant polypeptide of major histocompatibility complex, class II antigen-associated; leukocyte antigen; leukocyte antigen class II; leukocyte antigen DRB3; lymphocyte antigen DRB1; major histocompatibility complex class II DR-beta chain; major histocompatibility complex, class II, DR alpha; major histocompatibility complex, class II, DR beta 3; major histocompatibility complex, class II, DRB3; MHC cell surface glycoprotein; MHC class I antigen; MHC Class II antigen; MHC class II antigen beta chain; MHC class II antigen BoLA-DRB3; MHC class II antigen DR beta 3 chain; MHC class II antigen DRA; MHC class II antigen DRB1*9; MHC class II antigen DRB3; MHC class II antigen DRB4; MHC class II antigen DRB5; MHC class II antigen E alpha; MHC class II antigen HLA-DR-beta; MHC class II DR beta chain; MHC class II DR beta-chain; MHC class II DRB3; MHC class II HLA beta chain; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR beta 3 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; MHC class II leukocyte antigen BoLA-DRB3; MHC class II protein; MHC class II-associated invariant chain; MHC class2 antigen; MHC HLA DR-beta chain; MHC HLA-DR gamma chain; MHC HLA-DR-beta cell surface glycoprotein; MHC HLA-DR-beta chain; MHC(BoLA) class II DR-beta chain; p33; XXbac-BPG161M6.1
Gene Aliases: AI323765; BLA-DRB3; BOS_21567; BOS_248; BOS_8314; Bota-DRB01; Bota-DRB02; Bota-DRB04; Bota-DRB07; Bota-DRB21; Bota-DRB22; CD74; CLIP; DHLAG; DR-4; DR4; DRB1; DRB4; DRw10; E-alpha-f; H-2Ea; H2-Ea; H2-Ea-ps; HLA-DR1B; HLA-DR3B; HLA-DR4B; HLA-DRA; HLA-DRA1; HLA-DRB; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5; HLADG; I-Ealpha; Ia-3; Ia-GAMMA; Ia3; II; LA-DRB; MLRW; SS1
beta-amyloid binding
cytokine receptor activity
protein binding
cytokine binding
MHC class II protein complex binding
macrophage migration inhibitory factor binding
MHC class II protein binding
MHC class II protein binding, via antigen binding groove
identical protein binding
protein binding involved in protein folding
nitric-oxide synthase binding
MHC class II receptor activity
peptide antigen binding
antigen processing and presentation of exogenous peptide antigen via MHC class II
T cell costimulation
T cell receptor signaling pathway
interferon-gamma-mediated signaling pathway
activation of MAPK activity
prostaglandin biosynthetic process
positive regulation of cytokine-mediated signaling pathway
immune system process
positive regulation of dendritic cell antigen processing and presentation
negative regulation of peptide secretion
positive regulation of adaptive immune response
positive regulation of type 2 immune response
negative regulation of mature B cell apoptotic process
protein folding
protein complex assembly
intracellular protein transport
defense response
immune response
signal transduction
cell proliferation
immunoglobulin mediated immune response
cytokine-mediated signaling pathway
antigen processing and presentation
positive regulation of B cell proliferation
macrophage migration inhibitory factor signaling pathway
negative regulation of apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
positive thymic T cell selection
negative thymic T cell selection
positive regulation of innate immune response
negative regulation of T cell differentiation
positive regulation of T cell differentiation
positive regulation of fibroblast proliferation
positive regulation of peptidyl-tyrosine phosphorylation
chaperone mediated protein folding requiring cofactor
positive regulation of macrophage cytokine production
positive regulation of ERK1 and ERK2 cascade
positive regulation of neutrophil chemotaxis
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of chemokine (C-X-C motif) ligand 2 production
antigen processing and presentation of endogenous antigen
regulation of macrophage activation
T cell selection
leukocyte migration
peptide antigen assembly with MHC class II protein complex
antigen processing and presentation of peptide or polysaccharide antigen via MHC class II
polysaccharide assembly with MHC class II protein complex
viral process
cognition
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