Invitrogen

HLA-DR Monoclonal Antibody (L243), FITC, eBioscience™

HLA-DR Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 6

HLA-DR Antibody (11-9952-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (blue histogram) or Anti-Human HLA-DR FITC (purple histogram). Cells in the lymphocyte (left) and monocyte (right) gate were used for analysis.

Product Image Gallery

Product Details

11-9952-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Dog, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

L243

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2572542

Product Specific Information

Description: The L243 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4+ T lymphocytes. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported: This L243 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This L243 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant.

Cell genomics

Wang Q,Martínez-Bonet M,Kim T,Sparks JA,Ishigaki K,Chen X,Sudman M,Aguiar V,Sim S,Hernandez MC,Chiu DJ,Wactor A,Wauford B,Marion MC,Gutierrez-Arcelus M,Bowes J,Eyre S,Nordal E,Prahalad S,Rygg M,Videm V,Raychaudhuri S,Weirauch MT,Langefeld CD,Thompson SD,Nigrovic PA

11-9952-42 was used in Flow Cytometry to reveal that rs7034653, FRA2, and TRAF1 mediate a pathway through which a non-coding functional variant drives risk of inflammatory arthritis in children and adults.

Wed Nov 08 00:00:00 UTC 2023

NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses.

iScience

Tidu F,De Zuani M,Jose SS,Bendíčková K,Kubala L,Caruso F,Cavalieri F,Forte G,Frič J

11-9952-42 was used in Flow Cytometry to reveal a dual mechanism underlying the MSC response to zymosan: while NF-κB directly controls inflammatory cytokine expression, NFAT impacts immune-cell functions by regulating ECM remodeling.

Fri Jun 25 00:00:00 UTC 2021

Immune Landscape of Viral- and Carcinogen-Driven Head and Neck Cancer.

Immunity

Cillo AR,Kürten CHL,Tabib T,Qi Z,Onkar S,Wang T,Liu A,Duvvuri U,Kim S,Soose RJ,Oesterreich S,Chen W,Lafyatis R,Bruno TC,Ferris RL,Vignali DAA

11-9952 was used in Flow cytometry/Cell sorting to assess the transcriptional profiles of 131,224 single cells from peripheral and intra-tumoral immune populations from patients with HPV- and HPV+ HNSCC and healthy donors.

Tue Jan 14 00:00:00 UTC 2020

Macrophages induce malignant traits in mammary epithelium via IKKε/TBK1 kinases and the serine biosynthesis pathway.

EMBO molecular medicine

Wilcz-Villega E,Carter E,Ironside A,Xu R,Mataloni I,Holdsworth J,Jones W,Moreno Béjar R,Uhlik L,Bentham RB,Godinho SA,Dalli J,Grose R,Szabadkai G,Jones L,Hodivala-Dilke K,Bianchi K

11-9952-42 was used in Flow Cytometry to demonstrate that breast epithelial cells acquire malignant properties when exposed to medium conditioned by macrophages derived from human healthy donors.

Fri Feb 07 00:00:00 UTC 2020

Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis.

Cell reports

Ko JH,Kim HJ,Jeong HJ,Lee HJ,Oh JY

11-9952-42 was used in Flow Cytometry to demonstrate the role of mesenchymal stem and stromal cell and macrophage cross-talk in post-injury tissue homeostasis in mice.

Tue Mar 17 00:00:00 UTC 2020

Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy.

Scientific reports

Lee J,Sheen JH,Lim O,Lee Y,Ryu J,Shin D,Kim YY,Kim M

Published figure using HLA-DR monoclonal antibody (Product # 11-9952-42) in Flow Cytometry

Tue Oct 20 00:00:00 UTC 2020

Si-ni-tang (a Chinese herbal formula) for improving immunofunction in sepsis: study protocol for a pilot randomized controlled trial.

Trials

Zeng R,Zheng Y,Fan R,Zhou G,Zhang Y,Mai S,Xie D,Weng Y,Du J,Han Y,Lai F

11-9952 was used in Flow cytometry/Cell sorting to study whether a Chinese herbal treatment, Si-ni-tang, can improve immunofunction in sepsis patients.

Wed Aug 28 00:00:00 UTC 2019

In Vitro Expansion and Characterization of Mesenchymal Stromal Cells from Peritoneal Dialysis Effluent in a Human Protein Medium.

Stem cells international

Han B,Zhou L,Guan Q,da Roza G,Wang H,Du C

11-9952 was used in Flow cytometry/Cell sorting to show that upon expansion in a human protein medium, pMSCs shows a differential MSC marker expression profile from those of bone marrow or adipose tissue-derived MSCs and could maintain the multipotency.

Wed Sep 30 00:00:00 UTC 2020

Miniaturized High-Throughput Multiparameter Flow Cytometry Assays Measuring In Vitro Human Dendritic Cell Maturation and T-Cell Activation in Mixed Lymphocyte Reactions.

SLAS discovery : advancing life sciences R & D

Fan Y,Naglich JG,Koenitzer JD,Ribeiro H,Lippy J,Blum J,Li X,Milburn C,Barnhart B,Zhang L,Fereshteh MP

11-9952 was used in Flow cytometry/Cell sorting to monitor human dendritic cell maturation and lymphocyte proliferation in mixed lymphocyte reactions.

Wed Aug 01 00:00:00 UTC 2018

Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.

Oncology letters

Chen X,Wang Y,Wang J,Wen J,Jia X,Wang X,Zhang H

Published figure using HLA-DR monoclonal antibody (Product # 11-9952-42) in Flow Cytometry

Sun Jul 01 00:00:00 UTC 2018

Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.

Experimental and therapeutic medicine

Yan N,Xu J,Zhao C,Wu Y,Gao F,Li C,Zhou W,Xiao T,Zhou X,Shao Q,Xia S

Published figure using HLA-DR monoclonal antibody (Product # 11-9952-42) in Flow Cytometry

Sat Dec 01 00:00:00 UTC 2018

ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.

Frontiers in immunology

Lecciso M,Ocadlikova D,Sangaletti S,Trabanelli S,De Marchi E,Orioli E,Pegoraro A,Portararo P,Jandus C,Bontadini A,Redavid A,Salvestrini V,Romero P,Colombo MP,Di Virgilio F,Cavo M,Adinolfi E,Curti A

11-9952 was used in Flow cytometry/Cell sorting to study the effect of ATP release in acute myeloid lymphoma on the generation of an immune suppressive microenvironment.

Thu Mar 31 00:00:00 UTC 2022

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.

Stem cell research & therapy

Li J,Mao Q,He J,She H,Zhang Z,Yin C

11-9952 was used in Flow cytometry/Cell sorting to investigate the potential of hUCMSCs in improving ovarian function and withstanding ovarian senescence.

Thu Mar 09 00:00:00 UTC 2017

Human decidua mesenchymal stem cells regulate decidual natural killer cell function via interactions between collagen and leukocyte‑associated immunoglobulin‑like receptor 1.

Molecular medicine reports

Fu Q,Man X,Yu M,Chu Y,Luan X,Piao H,Xue J,Jin C

11-9952-42 was used in Flow cytometry/Cell sorting to identify mesenchymal stem cells (MSCs) from human first-trimester deciduas, termed decidual MSCs (DMSCs), and to investigate the effect of DMSCs on the regulation of dNK cells via collagen.

Fri Sep 01 00:00:00 UTC 2017

Remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions.

Proceedings of the National Academy of Sciences of the United States of America

Jönsson P,Southcombe JH,Santos AM,Huo J,Fernandes RA,McColl J,Lever M,Evans EJ,Hudson A,Chang VT,Hanke T,Godkin A,Dunne PD,Horrocks MH,Palayret M,Screaton GR,Petersen J,Rossjohn J,Fugger L,Dushek O,Xu XN,Davis SJ,Klenerman D

Published figure using HLA-DR monoclonal antibody (Product # 11-9952-42) in Flow Cytometry

Tue May 17 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: CD74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; DASS-397D15.1; DLA class II histocompatibility antigen, DR-1 beta chain; DLA DRBB1 beta chain; DLA-DR beta; DR beta-5; DR-9; DR2-beta-2; DR7; DR9; DRB1 transplantation antigen; Dw2; DW2.2/DR2.2; FLJ51114; FLJ75017; FLJ76359; gamma chain of class II antigens; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen gamma chain; HLA class II histocompatibility antigen, DR alpha chain; HLA class II histocompatibility antigen, DR beta 3 chain; HLA class II histocompatibility antigen, DR beta 4 chain; HLA class II histocompatibility antigen, DR beta 5 chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DR-5 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA class II histocompatibility antigen, DRB1-7 beta chain; HLA class II histocompatibility antigen, DRB1-9 beta chain; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; HLA-DRB1; HLA-DRB5; human leucocyte antigen DRB1; human leucocyte antigen DRB3; human leucocyte antigen DRB4; human leucocyte antigen DRB5; Human leukocyte antigen DRB1; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ii; leukocyte antigen; leukocyte antigen class II; lymphocyte antigen DRB1; MHC cell surface glycoprotein; MHC class I antigen; MHC Class II antigen; MHC class II antigen beta chain; MHC class II antigen DR beta 3 chain; MHC class II antigen DRA; MHC class II antigen DRB1*9; MHC class II antigen DRB3; MHC class II antigen DRB4; MHC class II antigen DRB5; MHC class II antigen HLA-DR-beta; MHC class II DLA-DR-beta-1; MHC class II DLA-DRB; MHC class II DR beta 1; MHC class II HLA beta chain; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR beta 3 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; MHC class2 antigen; MHC HLA DR-beta chain; MHC HLA-DR gamma chain; MHC HLA-DR-beta cell surface glycoprotein; MHC HLA-DR-beta chain; p33; XXbac-BPG161M6.1

View more View less

Gene Aliases: CD74; DHLAG; DLA-DRB1; DLA-DRBB1; DR-4; DR4; DRB; DRB1; DRB4; DRBB1; DRw10; HLA-DR1B; HLA-DR3B; HLA-DR4B; HLA-DRA; HLA-DRA1; HLA-DRB; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5; HLADG; Ia-GAMMA; II; LA-DRB; MLRW; SS1

View more View less

UniProt ID: (Human) P04233, (Human) P01903, (Dog) P18470, (Human) P01911, (Human) P01913, (Human) P13762, (Human) Q30154

View more View less

Entrez Gene ID: (Rhesus monkey) 710820, (Human) 972, (Cynomolgus monkey) 102131412, (Dog) 479329, (Human) 3122, (Dog) 474860, (Human) 3123, (Human) 3125, (Human) 3126, (Human) 3127

View more View less

Function(s)

beta-amyloid binding cytokine receptor activity protein binding cytokine binding MHC class II protein complex binding macrophage migration inhibitory factor binding MHC class II protein binding MHC class II protein binding, via antigen binding groove identical protein binding protein binding involved in protein folding nitric-oxide synthase binding MHC class II receptor activity peptide antigen binding

Process(es)

antigen processing and presentation of exogenous peptide antigen via MHC class II T cell costimulation T cell receptor signaling pathway interferon-gamma-mediated signaling pathway antigen processing and presentation of peptide or polysaccharide antigen via MHC class II immune response signal transduction activation of MAPK activity prostaglandin biosynthetic process positive regulation of cytokine-mediated signaling pathway positive regulation of dendritic cell antigen processing and presentation negative regulation of peptide secretion positive regulation of type 2 immune response negative regulation of mature B cell apoptotic process protein complex assembly intracellular protein transport defense response cell proliferation immunoglobulin mediated immune response antigen processing and presentation of endogenous antigen positive regulation of B cell proliferation macrophage migration inhibitory factor signaling pathway regulation of macrophage activation negative regulation of apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator T cell selection positive thymic T cell selection negative thymic T cell selection negative regulation of T cell differentiation positive regulation of T cell differentiation positive regulation of fibroblast proliferation positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration chaperone mediated protein folding requiring cofactor positive regulation of macrophage cytokine production positive regulation of ERK1 and ERK2 cascade positive regulation of neutrophil chemotaxis negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of chemokine (C-X-C motif) ligand 2 production peptide antigen assembly with MHC class II protein complex polysaccharide assembly with MHC class II protein complex viral process cognition

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

HLA-DR Monoclonal Antibody (L243), FITC, eBioscience™

HLA-DR Antibody (11-9952-42) in Flow

Product Details

11-9952-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!