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HLA-DR1 Antibody (MA1-19393) in WB
Western Blot analysis of HLA-DR1 using HLA-DR1 Monoclonal Antibody (MEM-267) (Product # MA1-19393). Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG2b monoclonal antibody (1 µg/mL), followed by Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was ... View More
Product Details
MA1-19393
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
MEM-267
Immunogen
Purified, insoluble DR1 beta chain, expressed in E. coli inclusion bodies.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1076828
Product Specific Information
This antibody binds extracellularly to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
For Flow cytometry: The antibody stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms.
Target Information
HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen presenting cells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DW2.2/DR2.2; FLJ75017; FLJ76359; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen, DR alpha chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA-DRB1; human leucocyte antigen DRB1; Human leukocyte antigen DRB1; lymphocyte antigen DRB1; MHC cell surface glycoprotein; MHC class II antigen; MHC class II antigen DRA; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; XXbac-BPG161M6.1
Gene Aliases: DRB1; DRw10; HLA-DR1B; HLA-DRA; HLA-DRA1; HLA-DRB; HLA-DRB1; MLRW; SS1
UniProt ID: (Human) P01903, (Human) P01911
Entrez Gene ID: (Human) 3122, (Human) 3123
peptide antigen assembly with MHC class II protein complex
antigen processing and presentation of peptide or polysaccharide antigen via MHC class II
polysaccharide assembly with MHC class II protein complex
immune response
viral process
antigen processing and presentation of exogenous peptide antigen via MHC class II
T cell costimulation
T cell receptor signaling pathway
cognition
interferon-gamma-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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