Proteintech

HLTF Polyclonal Antibody

HLTF Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

HLTF Antibody (14286-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 14286-1-AP (HLTF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

HLTF Antibody in Immunoprecipitation (IP)

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Product Details

14286-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HLTF Fusion Protein Ag5596 (1-354 aa encoded by BC044659)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSWMFKRDP VWKYLQTVQY GVHGNFPRLS YPTFFPRFEF QDVIPPDDFL TSDEEVDSVL FGSLRGHVVG LRYYTGVVNN NEMVALQRDP NNPYDKNAIK VNNVNGNQVG HLKKELAGAL AYIMDNKLAQ IEGVVPFGAN NAFTMPLHMT FWGKEENRKA VSDQLKKHGF KLGPAPKTLG FNLESGWGSG RAGPSYSMPV HAAVQMTTEQ LKTEFDKLFE DLKEDDKTHE MEPAEAIETP LLPHQKQALA WMVSRENSKE LPPFWEQRND LYYNTITNFS EKDRPENVHG GILADDMGLG KTLTAIAVIL TNFHDGRPLP IERVKKNLLK KEYNVNDDSM KLGGNNTSEK ADGLS (1-354 aa encoded by BC044659)

Target Information

This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HLTF promotes hepatocellular carcinoma progression by enhancing SRSF1 stability and activating ERK/MAPK pathway.

Oncogenesis

Xu Y,Ke S,Lu S,Wang C,Li Z,Feng Z,Yu H,Bai M,Qian B,Yin B,Li X,Hua Y,Jiang H,Ma Y

Fri Jan 20 00:00:00 UTC 2023

Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.

eLife

McMahon KA,Stroud DA,Gambin Y,Tillu V,Bastiani M,Sierecki E,Polinkovsky ME,Hall TE,Gomez GA,Wu Y,Parat MO,Martel N,Lo HP,Khanna KK,Alexandrov K,Daly R,Yap A,Ryan MT,Parton RG

Fri Jun 18 00:00:00 UTC 2021

Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses.

Frontiers in immunology

Gao W,Rui Y,Li G,Zhai C,Su J,Liu H,Zheng W,Zheng B,Zhang W,Yang Y,Hua S,Yu X

Wed Jan 05 00:00:00 UTC 2022

Break-induced replication promotes fragile telomere formation.

Genes & development

Yang Z,Takai KK,Lovejoy CA,de Lange T

Thu Oct 01 00:00:00 UTC 2020

HLTF suppresses the migration and invasion of colorectal cancer cells via TGF‑β/SMAD signaling in vitro.

International journal of oncology

Liu L,Liu H,Zhou Y,He J,Liu Q,Wang J,Zeng M,Yuan D,Tan F,Zhou Y,Pei H,Zhu H

Sat Dec 01 00:00:00 UTC 2018

HLTF suppresses the migration and invasion of colorectal cancer cells via TGF‑β/SMAD signaling in vitro.

International journal of oncology

Liu L,Liu H,Zhou Y,He J,Liu Q,Wang J,Zeng M,Yuan D,Tan F,Zhou Y,Pei H,Zhu H

Sat Dec 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: DNA-binding protein/plasminogen activator inhibitor 1 regulator; DNA-binding protein/plasminogen activator inhibitor-1 regulator; Helicase-like transcription factor; HIP116; mem; P113; RING finger protein 80; RING-type E3 ubiquitin transferase HLTF; SNF2-like 3; Sucrose nonfermenting protein 2-like 3; sucrose nonfermenting-like 3; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; TNF-response element-binding protein

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Gene Aliases: AF010600; BC057116; HIP116; HIP116A; HLTF; HLTF1; P113; RNF80; SMARCA3; SNF2L3; ZBU1

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UniProt ID: (Human) Q14527, (Mouse) Q6PCN7

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Entrez Gene ID: (Human) 6596, (Mouse) 20585, (Rat) 295568

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding helicase activity protein binding ATP binding zinc ion binding ligase activity ATPase activity ubiquitin protein ligase binding poly(A) RNA binding ubiquitin protein ligase activity nucleotide binding nucleic acid binding catalytic activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity hydrolase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

Process(es)

protein polyubiquitination postreplication repair regulation of transcription, DNA-templated transcription from RNA polymerase II promoter covalent chromatin modification positive regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription from RNA polymerase II promoter metabolic process chromatin modification

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HLTF Polyclonal Antibody

HLTF Antibody (14286-1-AP) in ICC/IF

Product Details

14286-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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