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Invitrogen
HMG4 Polyclonal Antibody
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FIGURE: 1 / 7
HMG4 Antibody (PA5-79374) in ICC/IF
Immunocytochemistry analysis of HMG4 using anti-HMG4 antibody (Product # PA5-79374). HMG4 was detected in a section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-HMG4 antibody (Product # PA5-79374) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
Product Details
PA5-79374
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HMG4 (62-95aa EMAKADKVRYDREMKDYGPAKGGKKKKDPNAPKR).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746490
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Mouse Liver Tissue, Mouse Kidney Tissue, Mouse Testis Tissue, 22RV1 whole cell, MCF-7 whole cell, NIH3T3 whole cell. IHC: mouse intestine tissue, rat intestine tissue, human placenta tissue. ICC/IF: MCF-7 cell. Flow: HL-60 cell.
Target Information
HMGB3 belongs to the high mobility group protein superfamily. Like HMG1 and HMG2, HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: high mobility group box 3; High mobility group protein 2a; High mobility group protein 4; High mobility group protein B3; high mobilty group protein 2A; high-mobility group (nonhistone chromosomal) protein 4; high-mobility group protein 4; HMG-2a; HMG-4; MGC90319
Gene Aliases: HMG-2a; HMG-4; HMG2A; HMG4; HMGB3; RGD1564407
UniProt ID: (Human) O15347, (Mouse) O54879
Entrez Gene ID: (Human) 3149, (Rat) 305373, (Mouse) 15354
chromatin remodeling
regulation of transcription, DNA-templated
negative regulation of B cell differentiation
negative regulation of myeloid cell differentiation
DNA recombination
transcription, DNA-templated
multicellular organism development
DNA geometric change
innate immune response
negative regulation of cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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