Invitrogen

HMGA1 Recombinant Rabbit Monoclonal Antibody (5J0E6)

View all (15) HMGA1 antibodies

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-35485

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:4,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000-1:8,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

ELISA (ELISA)

1 µg/mL
-

ChIP assay (ChIP)

3 µg antibody for 10 µg-15 µg of Chromatin
-

ChIP-sequencing (ChIP-Seq)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5J0E6

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-107 of human HMGA1 (P17096)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.6 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849386

Product Specific Information

Immunogen sequence: MSESSSKSSQ PLASKQEKDG TEKRGRGRPR KQPPVSPGTA LVGSQKEPSE VPTPKRPRGR PKGSKNKGAA KTRKTTTTPG RKPRGRPKKL EKEEEEGISQ ESSEEEQ

Target Information

HMGA1 encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: HGNC:5010; High mobility group AT-hook protein 1; High mobility group protein A1; High mobility group protein HMG-I/HMG-Y; high mobility group protein I; High mobility group protein R; high-mobility group (nonhistone chromosomal) protein isoforms I and Y; HMG-I(Y); MGC12816; MGC4242; MGC4854; nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y; OTTHUMP00000016222; OTTHUMP00000016223; OTTHUMP00000016224; OTTHUMP00000039618

View more View less

Gene Aliases: AL023995; HMG-R; HMGA1; HMGA1A; Hmga1b; Hmgi; HMGIY; Hmgy

View more View less

UniProt ID: (Human) P17096, (Mouse) P17095, (Rat) Q8K585

View more View less

Entrez Gene ID: (Human) 3159, (Mouse) 15361, (Rat) 117062

View more View less

Function(s)
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding AT DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding enzyme binding ligand-dependent nuclear receptor transcription coactivator activity retinoic acid receptor binding peroxisome proliferator activated receptor binding retinoid X receptor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA-(apurinic or apyrimidinic site) lyase activity 5'-deoxyribose-5-phosphate lyase activity
Process(es)
DNA unwinding involved in DNA replication nucleosome disassembly regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein complex assembly negative regulation of cell proliferation response to virus negative regulation of chromatin silencing senescence-associated heterochromatin focus assembly negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter nuclear transport establishment of integrated proviral latency oncogene-induced cell senescence positive regulation of cellular senescence DNA metabolic process base-excision repair spermatogenesis nucleic acid phosphodiester bond hydrolysis regulation of nucleic acid-templated transcription DNA catabolic process, endonucleolytic transcription, DNA-templated
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support