Invitrogen

HMGB1 Polyclonal Antibody

HMGB1 Antibody in Immunocytochemistry (ICC/IF)

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HMGB1 Antibody (PA1-16926) in ICC/IF

Immunofluorescence analysis of HMGB1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with HMGB1 Polyclonal Antibody (Product # PA1-16926) at 0.05 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3279... View More

HMGB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMGB1 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

HMGB1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

HMGB1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with HMGB1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-HMGB1 Polyclonal Antibody (Product # PA1-16926). {KD}

Product Details

PA1-16926

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:250

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

0.05 µg/mL

View 3 publications 3 publications

Flow Cytometry (Flow)

1:100

ELISA (ELISA)

Assay-Dependent

ChIP assay (ChIP)

5 µg/mL

Functional Assay (Functional)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Sheep, Rabbit, Rat

Published species

Dog, Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide between to amino acids 100-200 of the human HMGB1 protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2248274

Product Specific Information

This antibody is expected to cross react with porcine, equine and rabbit samples based on 100% sequence homology.

Suggested positive control: Hela whole cell extract, antigen standard for HMGB1 (transient overexpression lysate).

Target Information

HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4. HMGB1 is highly conserved and ubiquitous in the nuclei and cytoplasm of nearly all cell types, is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Down-syndrome-induced senescence disrupts the nuclear architecture of neural progenitors.

Cell stem cell

Meharena HS,Marco A,Dileep V,Lockshin ER,Akatsu GY,Mullahoo J,Watson LA,Ko T,Guerin LN,Abdurrob F,Rengarajan S,Papanastasiou M,Jaffe JD,Tsai LH

PA1-16926 was used in Immunocytochemistry to provide a mechanistic link between T21 and global transcriptional disruption and indicate that senescence-associated phenotypes may play a key role in the neurodevelopmental pathogenesis of DS.

Thu Jan 06 00:00:00 UTC 2022

Pentoxifylline prevents epileptic seizure via modulating HMGB1/RAGE/TLR4 signalling pathway and improves memory in pentylenetetrazol kindling rats.

Clinical and experimental pharmacology & physiology

Badawi GA,Shokr MM,Zaki HF,Mohamed AF

PA1-16926 was used in ChIP Assay, ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to find that PTX or its combination with phenytoin represent a promising drug to inhibit the epilepsy progression via targeting the HMGB1/TLR4/RAGE signalling pathway.

Sun Aug 01 00:00:00 UTC 2021

Pentoxifylline prevents epileptic seizure via modulating HMGB1/RAGE/TLR4 signalling pathway and improves memory in pentylenetetrazol kindling rats.

Clinical and experimental pharmacology & physiology

Badawi GA,Shokr MM,Zaki HF,Mohamed AF

Sun Aug 01 00:00:00 UTC 2021

The Potential of Nanobody-Targeted Photodynamic Therapy to Trigger Immune Responses.

Cancers

Beltrán Hernández I,Angelier ML,Del Buono D'Ondes T,Di Maggio A,Yu Y,Oliveira S

PA1-16926 was used in Immunocytochemistry-immunoflourescence to explore the potential of NB-PDT to stimulate the immune system.

Wed Apr 15 00:00:00 UTC 2020

The Potential of Nanobody-Targeted Photodynamic Therapy to Trigger Immune Responses.

Cancers

Beltrán Hernández I,Angelier ML,Del Buono D'Ondes T,Di Maggio A,Yu Y,Oliveira S

PA1-16926 was used in Immunocytochemistry-immunoflourescence to explore the potential of NB-PDT to stimulate the immune system.

Wed Apr 15 00:00:00 UTC 2020

A small bioactive glycoside inhibits epsilon toxin and prevents cell death.

Disease models & mechanisms

Shivappagowdar A,Pati S,Narayana C,Ayana R,Kaushik H,Sah R,Garg S,Khanna A,Kumari J,Garg L,Sagar R,Singh S

PA1-16926 was used in Functional assays to show a potent nontoxic glycoside-4 can protect mice from Clostridium perfringens epsilon toxin exposure.

Thu Oct 10 00:00:00 UTC 2019

A small bioactive glycoside inhibits epsilon toxin and prevents cell death.

Disease models & mechanisms

Shivappagowdar A,Pati S,Narayana C,Ayana R,Kaushik H,Sah R,Garg S,Khanna A,Kumari J,Garg L,Sagar R,Singh S

PA1-16926 was used in Functional assays to show a potent nontoxic glycoside-4 can protect mice from Clostridium perfringens epsilon toxin exposure.

Thu Oct 10 00:00:00 UTC 2019

Expression Analysis of Autophagy Related Markers LC3B, p62 and HMGB1 Indicate an Autophagy-Independent Negative Prognostic Impact of High p62 Expression in Pulmonary Squamous Cell Carcinomas.

Cancers

Langer R,Neppl C,Keller MD,Schmid RA,Tschan MP,Berezowska S

Published figure using HMGB1 polyclonal antibody (Product # PA1-16926) in Immunohistochemistry

Tue Aug 21 00:00:00 UTC 2018

The presence of LC3B puncta and HMGB1 expression in malignant cells correlate with the immune infiltrate in breast cancer.

Autophagy

Ladoire S,Enot D,Senovilla L,Ghiringhelli F,Poirier-Colame V,Chaba K,Semeraro M,Chaix M,Penault-Llorca F,Arnould L,Poillot ML,Arveux P,Delaloge S,Andre F,Zitvogel L,Kroemer G

PA1-16926 was used in immunohistochemistry - paraffin section to assess the correlation with the immune infiltration in breast cancer due to the presence of HMGB1and LC3B puncta expression in malignant cells

Tue May 03 00:00:00 UTC 2016

The presence of LC3B puncta and HMGB1 expression in malignant cells correlate with the immune infiltrate in breast cancer.

Autophagy

Ladoire S,Enot D,Senovilla L,Ghiringhelli F,Poirier-Colame V,Chaba K,Semeraro M,Chaix M,Penault-Llorca F,Arnould L,Poillot ML,Arveux P,Delaloge S,Andre F,Zitvogel L,Kroemer G

Tue May 03 00:00:00 UTC 2016

Biomarkers of immunogenic stress in metastases from melanoma patients: Correlations with the immune infiltrate.

Oncoimmunology

Ladoire S,Senovilla L,Enot D,Ghiringhelli F,Poirier-Colame V,Chaba K,Erdag G,Schaefer JT,Deacon DH,Zitvogel L,Slingluff CL,Kroemer G

PA116926 was used in immunohistochemistry - paraffin section to analyze immunogenic biomarkers and immune cells infiltrarion and prognosis in metastasis.

Wed Jun 01 00:00:00 UTC 2016

Combined evaluation of LC3B puncta and HMGB1 expression predicts residual risk of relapse after adjuvant chemotherapy in breast cancer.

Autophagy

Ladoire S,Penault-Llorca F,Senovilla L,Dalban C,Enot D,Locher C,Prada N,Poirier-Colame V,Chaba K,Arnould L,Ghiringhelli F,Fumoleau P,Spielmann M,Delaloge S,Poillot ML,Arveux P,Goubar A,Andre F,Zitvogel L,Kroemer G

PA1-16926 was used in immunohistochemistry - paraffin section to predict residual risk of relapse after adjuvant chemotherapy in breast cancer by combined evaluation of HMGB1 and LC3B puncta

Tue Sep 13 00:00:00 UTC 2016

Combined evaluation of LC3B puncta and HMGB1 expression predicts residual risk of relapse after adjuvant chemotherapy in breast cancer.

Autophagy

PA1-16926 was used in immunohistochemistry - paraffin section to predict residual risk of relapse after adjuvant chemotherapy in breast cancer by combined evaluation of HMGB1 and LC3B puncta

Tue Sep 13 00:00:00 UTC 2016

Negative prognostic impact of regulatory T cell infiltration in surgically resected esophageal cancer post-radiochemotherapy.

Oncotarget

Vacchelli E,Semeraro M,Enot DP,Chaba K,Poirier Colame V,Dartigues P,Perier A,Villa I,Rusakiewicz S,Gronnier C,Goéré D,Mariette C,Zitvogel L,Kroemer G

PA1-16926 was used in immunohistochemistry - paraffin section to examine the immune responses in patients with esophageal carcinomas treated by neo-adjuvant radiochemotherapy

Fri Aug 28 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Amphoterin; Amphoterin antibody; DKFZp686A04236; Heparin-binding protein p30; high mobility group 1; High mobility group protein 1; High mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; HMG-1; HMG3 antibody; hmgb 1; hmgb-1; RP11-550P23.1; Sulfoglucuronyl carbohydrate binding protein

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Gene Aliases: Ac2-008; amphoterin; BOS_12315; DEF; HMG-1; HMG1; HMG3; HMGB1; p30; SBP-1

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UniProt ID: (Human) P09429, (Bovine) P10103, (Dog) Q6YKA4, (Rat) P63159, (Mouse) P63158

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Entrez Gene ID: (Human) 3146, (Rabbit) 100328641, (Bovine) 282691, (Sheep) 101112071, (Dog) 403170, (Rat) 25459, (Mouse) 15289

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Function(s)

four-way junction DNA binding bubble DNA binding lipopolysaccharide binding phosphatidylserine binding damaged DNA binding double-stranded DNA binding single-stranded DNA binding transcription factor activity, sequence-specific DNA binding double-stranded RNA binding single-stranded RNA binding cytokine activity protein binding transcription factor binding DNA binding, bending calcium-dependent protein kinase regulator activity lyase activity C-X-C chemokine binding protein kinase activator activity chemoattractant activity poly(A) RNA binding RAGE receptor binding DNA polymerase binding repressing transcription factor binding supercoiled DNA binding histone binding non-sequence-specific DNA binding, bending open form four-way junction DNA binding crossed form four-way junction DNA binding DNA binding bent DNA binding chromatin binding 5S rRNA binding heparin binding peptide binding protein dimerization activity glycolipid binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HMGB1 Polyclonal Antibody

HMGB1 Antibody (PA1-16926) in ICC/IF

Product Details

PA1-16926

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

ChIP assay (ChIP)

Functional Assay (Functional)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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